{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:14:21Z","timestamp":1787498061572,"version":"build-2736575974"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Natural Science Foundation of Shandong Province, China","award":["ZR2020MC127"],"award-info":[{"award-number":["ZR2020MC127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The fall armyworm, Spodoptera frugiperda, a severe crop pest, gained significant attention following its invasion of China in 2019. This study aimed to investigate the virulence and in vivo pathogenic transition of Metarhizium rileyi on S. frugiperda to understand biological control strategies. Using the immersion method, M. rileyi exhibited higher lethality toward third-instar larvae compared to fourth-instar larvae, with LC50 values of 3.15\u2005\u00d7\u2005107 conidia\/ml and 1.04\u2005\u00d7\u2005108 conidia\/ml, respectively. Microscopic observations revealed that M. rileyi initially existed as a yeast-like nonpathogenic form within the host but later transformed into a mycelial-like pathogenic form to effectively kill the host insect. De novo transcriptome analysis identified 89,498 unigenes. KEGG analysis highlighted genes associated with \u201cmetabolic pathways\u201d and \u201cquorum sensing.\u201d Differentially expressed gene (DEG) analysis identified 2,988 DEGs during the morphological transformation of M. rileyi, with increased tyrosine levels due to 13 down-regulated genes in the \u201cbiosynthesis of various plant secondary metabolites\u201d pathway. DAHP synthase, an up-regulated enzyme from the quorum sensing pathway, also facilitated tyrosine biosynthesis. Given tyrosine\u2019s role as a precursor to tyrosol, we hypothesized tyrosol served as quorum sensing molecule regulating the pathogenic switch of M. rileyi. The hypothesis was validated in vitro, confirming 1000 mg\/L as the effective inducible concentration.<\/jats:p>","DOI":"10.1093\/jee\/toaf144","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T07:48:38Z","timestamp":1749196118000},"page":"1565-1578","source":"Crossref","is-referenced-by-count":2,"title":["Toxicity assessment and transcriptomic analysis of\n                    <i>Metarhizium rileyi<\/i>\n                    (Hypocreales: Clavicipitaceae) against\n                    <i>Spodoptera frugiperda<\/i>\n                    (Lepidoptera: Noctuidae): identification of tyrosol as a key quorum sensing regulator of pathogenicity"],"prefix":"10.1093","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2529-4274","authenticated-orcid":false,"given":"Shouzhu","family":"Liu","sequence":"first","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University , Liaocheng ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyi","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University , Liaocheng ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xipeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University , Liaocheng ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanhui","family":"Hao","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Shandong Agricultural University , Taian ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuhao","family":"Guo","sequence":"additional","affiliation":[{"name":"Liaocheng Agricultural and Rural Development Service Center, Liaocheng Agricultural and Rural Affairs Bureau , Liaocheng ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biology, Liaocheng University , Liaocheng ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,7,2]]},"reference":[{"key":"2025090509515231100_CIT0001","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"Abbott","year":"1925","journal-title":"J. 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