{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:47:24Z","timestamp":1790948844013,"version":"4.1.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004493","name":"International Atomic Energy Agency","doi-asserted-by":"publisher","award":["IAEA\/MEX\/16037"],"award-info":[{"award-number":["IAEA\/MEX\/16037"]}],"id":[{"id":"10.13039\/501100004493","id-type":"DOI","asserted-by":"publisher"}]},{"name":"SECHCITI","award":["669604"],"award-info":[{"award-number":["669604"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ionizing radiation is widely used in insect sterilization for pest control using the Sterile Insect Technique, which consists of the mass rearing of insects and their irradiation with gamma rays to release them in target areas where they will mate with wild females. However, there is a concern and controversy about the nuclear origin applied in this technique. One alternative for sterilization is the use of X-rays, which do not have a nuclear origin, are easier to operate, and do not generate radioactive waste. Here, we compared the sexual competitiveness of Mexican fruit fly, Anastrepha ludens (Loew) (Diptera: Tephritidae) males irradiated with gamma or X-radiation, and their sperm transfer ability. Mating frequency was similar between gamma and X-ray-irradiated males, but higher for wild males. However, the amount of sperm in females mated with X-ray-irradiated males was lower than that of females mated with gamma-irradiated males. Males irradiated with X-rays, mating for a third consecutive time, transferred fewer sperm than males irradiated with gamma rays. Females mating with X-ray irradiated males were more likely to remate than females mated to gamma irradiated or wild males. Overall, males sterilized with X-rays were as sexually competitive as males sterilized with gamma radiation; however, lower sperm transfer and higher remating with females suggest the need to perform dosimetries for X ray-treated males at a lower dose to increase performance. X-rays could be used as an alternative to gamma rays in the application of the SIT for A. ludens. Further research into the effects of X-rays on the male ejaculate, female fertility, and remating is needed.<\/jats:p>","DOI":"10.1093\/jee\/toaf186","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T13:14:55Z","timestamp":1752585295000},"page":"737-745","source":"Crossref","is-referenced-by-count":1,"title":["Gamma and X-ray radiation on mating and sperm transfer in sterile\n                    <i>Anastrepha ludens<\/i>\n                    (Diptera: Tephritidae)"],"prefix":"10.1093","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4778-4944","authenticated-orcid":false,"given":"Yeudiel","family":"G\u00f3mez-Simuta","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Agr\u00edcolas, Posgrado en Ciencias Agropecuarias, Universidad Veracruzana , Xalapa, Veracruz,","place":["Mexico"]},{"name":"Programa Nacional de Moscas de la Fruta, Programa operativo de moscas, SADER-SENASICA , Tapachula, Chiapas,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6804-0346","authenticated-orcid":false,"given":"Diana","family":"P\u00e9rez-Staples","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda y Ecolog\u00eda Aplicada, Universidad Veracruzana , Xalapa, Veracruz,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2137-6587","authenticated-orcid":false,"given":"Francisco","family":"D\u00edaz-Fleischer","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda y Ecolog\u00eda Aplicada, Universidad Veracruzana , Xalapa, Veracruz,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2066-9524","authenticated-orcid":false,"given":"Luis","family":"Quintero-Fong","sequence":"additional","affiliation":[{"name":"Programa Nacional de Moscas de la Fruta, Programa operativo de moscas, SADER-SENASICA , Tapachula, Chiapas,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6806-0945","authenticated-orcid":false,"given":"Dina","family":"Orozco-D\u00e1vila","sequence":"additional","affiliation":[{"name":"Instituto de Biociencias, Universidad Aut\u00f3noma de Chiapas , Tapachula, Chiapas,","place":["Mexico"]},{"name":"Departamento de Ecolog\u00eda de Artr\u00f3podos y Manejo de Plagas, El Colegio de la Frontera Sur , Tapachula, Chiapas,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0502-117X","authenticated-orcid":false,"given":"Yair","family":"Contreras-Navarro","sequence":"additional","affiliation":[{"name":"Programa Nacional de Moscas de la Fruta, Programa operativo de moscas, SADER-SENASICA , Tapachula, Chiapas,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9569-6079","authenticated-orcid":false,"given":"Jorge","family":"Toledo","sequence":"additional","affiliation":[{"name":"Departamento de Ecolog\u00eda de Artr\u00f3podos y Manejo de Plagas, El Colegio de la Frontera Sur , Tapachula, Chiapas,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"2026041316520618100_toaf186-B1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jinsphys.2016.03.001","article-title":"The male ejaculate as inhibitor of female remating in two tephritid flies","volume":"88","author":"Abraham","year":"2016","journal-title":"J. Insect Physiol"},{"key":"2026041316520618100_toaf186-B2","first-page":"233","volume-title":"Sterile insect technique, principles and practices in area-wide integrated pest management","author":"Bakri","year":"2005"},{"key":"2026041316520618100_toaf186-B3","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/978-1-4757-2278-9_36","volume-title":"Fruit flies: biology and management","author":"Bloem","year":"1993"},{"key":"2026041316520618100_toaf186-B4","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/1-4020-4051-2_10","volume-title":"Sterile insect technique, principles and practice in area-wide integrated pest management","author":"Calkins","year":"2005"},{"key":"2026041316520618100_toaf186-B5","first-page":"843","volume-title":"Fruit flies (tephritidae): phylogeny and evolution of behavior","author":"Cayol","year":"2000"},{"key":"2026041316520618100_toaf186-B6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jinsphys.2015.03.002","article-title":"Pupal X-ray irradiation influences protein expression in adults of the oriental fruit fly, Bactrocera dorsalis","volume":"76","author":"Chang","year":"2015","journal-title":"J. Insect Physiol"},{"key":"2026041316520618100_toaf186-B7","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toaf030","article-title":"X-rays and gamma rays do not differ in their effectiveness for sterilizing pupae and adults of the mosquito Aedes aegypti (Diptera: Culicidae)","author":"Chen","year":"2025","journal-title":"J. Econ. Entomol"},{"key":"2026041316520618100_toaf186-B8","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1093\/jee\/toaa178","article-title":"Pre- and post-copulatory competitiveness of the genetic sexing strain Tapachula-7 of Anastrepha ludens (Diptera: Tephritidae)","volume":"113","author":"Contreras-Navarro","year":"2020","journal-title":"J. Econ. Entomol."},{"key":"2026041316520618100_toaf186-B9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jinsphys.2024.104699","article-title":"Male condition and seminal fluid affect female host-marking behavior in the Mexican fruit fly","volume":"158","author":"C\u00f3rdova-Garc\u00eda","year":"2024","journal-title":"J. Insect Physiol."},{"key":"2026041316520618100_toaf186-B10","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1080\/07924259.2024.2304347","article-title":"Evaluation of the efficacy of X-ray irradiation on male testes of Mediterranean fruit fly using light and transmission electron microscopy","volume":"68","author":"Elelimy","year":"2024","journal-title":"Inv. Repro. Dev"},{"key":"2026041316520618100_toaf186-B11","volume-title":"Product quality control for sterile mass-reared and released Tephritid fruit flies, version 6.0","author":"FAO\/IAEA\/USDA","year":"2014"},{"key":"2026041316520618100_toaf186-B22","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1093\/jee\/tox326","article-title":"Sexual competitiveness, field survival, and dispersal of Anastrepha obliqua (Diptera: Tephritidae) fruit flies irradiated at different doses","volume":"111","author":"Gallardo","year":"2018","journal-title":"J. Econ. Entomol"},{"key":"2026041316520618100_toaf186-B12","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1653\/024.094.0443","article-title":"Fitness and reproductive potential of irradiated mass-reared Mediterranean fruit fly males Ceratitis capitata (Diptera: Tephritidae): lowering radiation doses","volume":"94","author":"Guerfali","year":"2011","journal-title":"Fla. Entomol"},{"key":"2026041316520618100_toaf186-B13","author":"International Atomic Energy Agency","year":"2012"},{"key":"2026041316520618100_toaf186-B14","author":"International Atomic Energy Agency","year":"2013"},{"key":"2026041316520618100_toaf186-B15","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/BF02350002","article-title":"Remating inhibition in the melon fly, Bactrocera (=Dacus) cucurbitae (Diptera: Tephritidae): copulation with spermless males inhibits female remating","volume":"11","author":"Kuba","year":"1993","journal-title":"J. Ethol"},{"key":"2026041316520618100_toaf186-B16","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1007\/s13355-018-0578-4","article-title":"Effects of X-ray irradiation on male sperm transfer ability and fertility in the sweetpotato weevils Euscepes postfasciatus (Coleoptera: Curculionidae) and Cylas formicarius (Coleoptera: Brentidae)","volume":"53","author":"Kumano","year":"2018","journal-title":"Appl. Entomol. Zool"},{"key":"2026041316520618100_toaf186-B17","first-page":"357","volume-title":"Moscas de la fruta: fundamentos y procedimientos Para su manejo","author":"Liedo","year":"2020"},{"key":"2026041316520618100_toaf186-B18","doi-asserted-by":"publisher","DOI":"10.5772\/21531","volume-title":"Radioisotopes-Applications in Bio-Medical Science","author":"Mastrangelo","year":"2011"},{"key":"2026041316520618100_toaf186-B19","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1080\/09583150802417849","article-title":"Radiation sources supporting the use of natural enemies for biological control of agricultural pests","volume":"19","author":"Mehta","year":"2009","journal-title":"Biocontrol Sci. Technol"},{"key":"2026041316520618100_toaf186-B20","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/S0022-1910(03)00027-1","article-title":"Regulation of sexual receptivity of female Mediterranean fruit flies: old hypotheses revisited and a new synthesis proposed","volume":"49","author":"Mossinson","year":"2003","journal-title":"J. Insect Physiol"},{"key":"2026041316520618100_toaf186-B21","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1111\/eea.12581","article-title":"Mass rearing and sterile insect releases for the control of Anastrepha spp. pests in Mexico\u2014a review","volume":"164","author":"Orozco-D\u00e1vila","year":"2017","journal-title":"Entomol. Exp. Appl"},{"key":"2026041316520618100_toaf186-B23","first-page":"209","volume-title":"Sterile insect technique, principles and practice in area-wide integradted pest management","author":"Parker","year":"2005"},{"key":"2026041316520618100_toaf186-B24","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1146\/annurev-ento-120220-113618","article-title":"Postcopulatory behavior of Tephritid flies","volume":"68","author":"P\u00e9rez-Staples","year":"2023","journal-title":"Annu. Rev. Entomol"},{"key":"2026041316520618100_toaf186-B25","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1570-7458.2012.01312.x","article-title":"Female mating failure and the failure of \u2018mating\u2019 in sterile insect programs","volume":"146","author":"P\u00e9rez-Staples","year":"2013","journal-title":"Entomol. Exp. 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Sci"},{"key":"2026041316520618100_toaf186-B31","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1046\/j.1570-7458.2001.00753.x","article-title":"Age-dependent insemination success of sterile Mediterranean fruit flies","volume":"98","author":"Taylor","year":"2001","journal-title":"Entomologia Exp. Applicata"},{"key":"2026041316520618100_toaf186-B32","doi-asserted-by":"publisher","first-page":"811","DOI":"10.1603\/ME13223","article-title":"X-ray-induced sterility in Aedes albopictus (Diptera: Culicidae) and male longevity following irradiation","volume":"51","author":"Yamada","year":"2014","journal-title":"J. Med. Entomol"},{"key":"2026041316520618100_toaf186-B33","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1186\/s12915-023-01757-1","article-title":"Different mechanisms of X-ray irradiation-induced male and female sterility in Aedes aegypti","volume":"21","author":"Zhang","year":"2023","journal-title":"BMC Biol."}],"container-title":["Journal of Economic Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jee\/article-pdf\/119\/2\/737\/64233124\/toaf186.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jee\/article-pdf\/119\/2\/737\/64233124\/toaf186.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T20:52:17Z","timestamp":1776113537000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jee\/article\/119\/2\/737\/8250206"}},"subtitle":[],"editor":[{"given":"Marion","family":"Le Gall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,9]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,9,9]]},"published-print":{"date-parts":[[2026,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jee\/toaf186","relation":{},"ISSN":["0022-0493","1938-291X"],"issn-type":[{"value":"0022-0493","type":"print"},{"value":"1938-291X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,4]]},"published":{"date-parts":[[2025,9,9]]}}}