{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T07:29:56Z","timestamp":1786519796791,"version":"build-2736575974"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Laimburg Research Centre and the Free University of Bozen-Bolzano, with financial support from the Autonomous Province of Bolzano\/Bozen"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Effective management of the fruit pest Drosophila suzukii Matsumura (Diptera: Drosophilidae) requires new strategies to reduce insecticide use and minimize their negative impact on beneficial organisms. This study evaluated the effectiveness of Saccharomycopsis vini supernatant, a formulation that retains attractive cues while offering improved storage and handling conditions compared to the non-sterile yeast suspension. In recent years, yeast-based attract-and-kill approaches have shown strong potential, with Hanseniaspora uvarum widely studied as an attractant. However, little attention has been given to alternative yeast species and the use of yeast supernatants rather than live cultures. In this study, we assessed for the first time the attractiveness of S. vini supernatant alongside H. uvarum suspension. In a Y-maze olfactometer bioassay, both formulations were attractive to D. suzukii females. Field trials in a vineyard and cherry orchards confirmed the efficacy of both yeast formulations when combined with spinosad. Importantly, the D. suzukii parasitoid Ganaspis kimorum Buffington (Hymenoptera: Figitidae) did not show increased mortality relative to spinosad alone. In conclusion, S. vini supernatant proved to be as effective as H. uvarum suspension in attracting D. suzukii, while showing no increase in parasitoid mortality compared with spinosad alone under laboratory conditions. These results highlight S. vini as a promising component of yeast-based attract-and-kill strategies and provide a solid basis for developing selective and sustainable management tools for D. suzukii.<\/jats:p>","DOI":"10.1093\/jee\/toag165","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T11:39:57Z","timestamp":1779968397000},"page":"2765-2776","source":"Crossref","is-referenced-by-count":0,"title":["Exploring\n                    <i>Saccharomycopsis vini<\/i>\n                    as a novel parasitoid-friendly attractant for efficient attract-and-kill of\n                    <i>Drosophila suzukii<\/i>\n                    (Diptera: Drosophilidae)"],"prefix":"10.1093","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2667-1924","authenticated-orcid":false,"given":"Marko","family":"Bjeljac","sequence":"first","affiliation":[{"name":"Institute for Plant Health, Laimburg Research Centre , Ora (Auer),","place":["Italy"]},{"name":"Faculty of Agricultural, Environmental and Food Sciences, Free University of Bozen-Bolzano , Bolzano,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0492-6129","authenticated-orcid":false,"given":"Urban","family":"Spitaler","sequence":"additional","affiliation":[{"name":"Institute for Plant Health, Laimburg Research Centre , Ora (Auer),","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8339-5540","authenticated-orcid":false,"given":"Claire","family":"Dum\u00e9nil","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural, Environmental and Food Sciences, Free University of Bozen-Bolzano , Bolzano,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Neulichedl","sequence":"additional","affiliation":[{"name":"Institute for Plant Health, Laimburg Research Centre , Ora (Auer),","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8295-1536","authenticated-orcid":false,"given":"Silvia","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Institute for Plant Health, Laimburg Research Centre , Ora (Auer),","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8463-7476","authenticated-orcid":false,"given":"Sergio","family":"Angeli","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural, Environmental and Food Sciences, Free University of Bozen-Bolzano , Bolzano,","place":["Italy"]},{"name":"Competence Centre for Plant Health, Free University of Bozen-Bolzano , Bolzano,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,6,16]]},"reference":[{"key":"2026081202533795100_toag165-B1","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"Abbott","year":"1925","journal-title":"J. Econ. Entomol"},{"key":"2026081202533795100_toag165-B2","doi-asserted-by":"publisher","first-page":"102067","DOI":"10.1016\/j.jafr.2025.102067","article-title":"Use of antagonistic yeasts for multi-purpose grape and wine protection: attraction for Drosophila suzukii in pre-harvest and bioprotection during red winemaking","volume":"22","author":"Arpellino","year":"2025","journal-title":"J. Agric. Food Res"},{"key":"2026081202533795100_toag165-B3","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1093\/jee\/toab273","article-title":"Factors influencing the efficacy of novel attract-and-kill (ACTTRA SWD) formulations against Drosophila suzukii","volume":"115","author":"Babu","year":"2022","journal-title":"J. Econ. Entomol"},{"key":"2026081202533795100_toag165-B4","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1111\/j.1365-2435.2012.02006.x","article-title":"Yeast, not fruit volatiles mediate Drosophila melanogaster attraction, oviposition and development","volume":"26","author":"Becher","year":"2012","journal-title":"Funct. Ecol"},{"key":"2026081202533795100_toag165-B5","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1007\/s10340-017-0932-2","article-title":"Dietary yeast affects preference and performance in Drosophila suzukii","volume":"91","author":"Bellutti","year":"2018","journal-title":"J. Pest Sci. (2004)"},{"key":"2026081202533795100_toag165-B6","doi-asserted-by":"publisher","first-page":"810","DOI":"10.3390\/insects11110810","article-title":"Persistence of a yeast-based (Hanseniaspora uvarum) attract-and-kill formulation against Drosophila suzukii on grape leaves","volume":"11","author":"Bianchi","year":"2020","journal-title":"Insects"},{"key":"2026081202533795100_toag165-B7","doi-asserted-by":"publisher","first-page":"360","DOI":"10.3390\/horticulturae7100360","article-title":"Changes in the fungal community assembly of apple fruit following postharvest application of the yeast biocontrol agent Metschnikowia fructicola","volume":"7","author":"Biasi","year":"2021","journal-title":"Horticulturae"},{"key":"2026081202533795100_toag165-B8","doi-asserted-by":"publisher","first-page":"106868","DOI":"10.1016\/j.cropro.2024.106868","article-title":"Canopy strip applications of Hanseniaspora uvarum combined with spinosad reduce insecticide use without compromising Drosophila suzukii control in cherry","volume":"186","author":"Bjeljac","year":"2024","journal-title":"Crop Prot"},{"key":"2026081202533795100_toag165-B9","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1007\/s10886-024-01494-x","article-title":"Chemical and electrophysiological characterisation of headspace volatiles from yeasts attractive to Drosophila suzukii","volume":"50","author":"Castellan","year":"2024","journal-title":"J. Chem. Ecol"},{"key":"2026081202533795100_toag165-B10","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1002\/ps.3568","article-title":"A four-component synthetic attractant for Drosophila suzukii (Diptera: Drosophilidae) isolated from fermented bait headspace","volume":"70","author":"Cha","year":"2014","journal-title":"Pest Manag. Sci"},{"key":"2026081202533795100_toag165-B11","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1007\/s10340-016-0740-0","article-title":"First exploration of parasitoids of Drosophila suzukii in South Korea as potential classical biological agents","volume":"89","author":"Daane","year":"2016","journal-title":"J. Pest Sci"},{"key":"2026081202533795100_toag165-B12","doi-asserted-by":"publisher","first-page":"89","DOI":"10.3233\/JBR-150092","article-title":"The economic impact of invasive pest Drosophila suzukii on berry production in the Province of Trento, Italy","volume":"5","author":"De Ros","year":"2015","journal-title":"J. Berry Res"},{"key":"2026081202533795100_toag165-B13","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1146\/annurev.ento.52.110405.091440","article-title":"The sublethal effects of pesticides on beneficial arthropods","volume":"52","author":"Desneux","year":"2007","journal-title":"Annu. Rev. Entomol"},{"key":"2026081202533795100_toag165-B14","doi-asserted-by":"publisher","first-page":"e0323653","DOI":"10.1371\/journal.pone.0323653","article-title":"Yeast-based attract-and-kill strategies for Drosophila suzukii management without disrupting honey bee activity","volume":"20","author":"Dum\u00e9nil","year":"2025","journal-title":"PLoS One"},{"key":"2026081202533795100_toag165-B15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10526-022-10174-2","article-title":"First report on classical biological control releases of the larval parasitoid Ganaspis brasiliensis against Drosophila suzukii in northern Italy","volume":"68","author":"Fellin","year":"2023","journal-title":"Bio. Control"},{"key":"2026081202533795100_toag165-B16","doi-asserted-by":"publisher","first-page":"105801","DOI":"10.1016\/j.biocontrol.2025.105801","article-title":"Investigating the overwintering strategy of Ganaspis kimorum, a biological control agent of Drosophila suzukii","volume":"206","author":"Fellin","year":"2025","journal-title":"Biol. Control"},{"key":"2026081202533795100_toag165-B17","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1111\/jen.12496","article-title":"Development of Asian parasitoids in larvae of Drosophila suzukii feeding on blueberry and artificial diet","volume":"142","author":"Girod","year":"2018","journal-title":"J. Applied Entomology"},{"key":"2026081202533795100_toag165-B18","first-page":"121","article-title":"Drosophila (Sophophora) suzukii (Matsumura), new pest of soft fruits in Trentino (North-Italy) and in Europe","volume":"70","author":"Grassi","year":"2011","journal-title":"IOBC\/WPRS Bull"},{"key":"2026081202533795100_toag165-B19","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1146\/annurev-ento-032725-033103","article-title":"The side effects of pesticides on nontarget arthropods","volume":"71","author":"Guedes","year":"2026","journal-title":"Annu. Rev. Entomol"},{"key":"2026081202533795100_toag165-B20","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1007\/s10340-016-0768-1","article-title":"Current knowledge of interactions between Drosophila suzukii and microbes, and their potential utility for pest management","volume":"89","author":"Hamby","year":"2016","journal-title":"J. Pest Sci"},{"key":"2026081202533795100_toag165-B21","doi-asserted-by":"publisher","first-page":"4869","DOI":"10.1128\/AEM.00841-12","article-title":"Associations of yeasts with spotted-wing drosophila (Drosophila suzukii; Diptera: Drosophilidae) in Cherries and Raspberries","volume":"78","author":"Hamby","year":"2012","journal-title":"Appl. Environ. Microbiol"},{"key":"2026081202533795100_toag165-B22","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1007\/s10340-016-0737-8","article-title":"Current SWD IPM tactics and their practical implementation in fruit crops across different regions around the world","volume":"89","author":"Haye","year":"2016","journal-title":"J. Pest Sci"},{"key":"2026081202533795100_toag165-B23","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1017\/CBO9780511542220.010","volume-title":"Plant-provided food for carnivorous insects: a protective mutualism and its applications","author":"Heimpel","year":"2005"},{"key":"2026081202533795100_toag165-B24","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1002\/bimj.200810425","article-title":"Simultaneous inference in general parametric models","volume":"50","author":"Hothorn","year":"2008","journal-title":"Biom. J"},{"key":"2026081202533795100_toag165-B25","doi-asserted-by":"publisher","first-page":"995","DOI":"10.3390\/insects13110995","article-title":"The efficacy of yeast phagostimulant baits in attract-and-kill strategies varies between summer- and winter-morphs of Drosophila suzukii","volume":"13","author":"Jones","year":"2022","journal-title":"Insects"},{"key":"2026081202533795100_toag165-B26","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1038\/s41598-020-79691-3","article-title":"Separate and combined Hanseniaspora uvarum and Metschnikowia pulcherrima metabolic volatiles are attractive to Drosophila suzukii in the laboratory and field","volume":"11","author":"Jones","year":"2021","journal-title":"Sci. Rep"},{"key":"2026081202533795100_toag165-B27","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1080\/01621459.1958.10501452","article-title":"Nonparametric estimation from incomplete observations","volume":"53","author":"Kaplan","year":"1958","journal-title":"J. Am. Stat. Assoc"},{"key":"2026081202533795100_toag165-B28","doi-asserted-by":"publisher","author":"Kassambara","year":"2024","DOI":"10.32614\/CRAN.package.survminer"},{"key":"2026081202533795100_toag165-B29","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1093\/jee\/toac029","article-title":"Hanseniaspora uvarum attracts Drosophila suzukii (Diptera: Drosophilidae) with high specificity","volume":"115","author":"Kleman","year":"2022","journal-title":"J. Econ. Entomol"},{"key":"2026081202533795100_toag165-B30","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1093\/jisesa\/iey132","article-title":"Testing a novel attract-and-kill strategy for Drosophila suzukii (Diptera: Drosophilidae) management","volume":"19","author":"Klick","year":"2019","journal-title":"J. Insect Sci"},{"key":"2026081202533795100_toag165-B31","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1111\/jen.12234","article-title":"Distribution and activity of Drosophila suzukii in cultivated raspberry and surrounding vegetation","volume":"140","author":"Klick","year":"2016","journal-title":"J. Applied Entomology"},{"key":"2026081202533795100_toag165-B32","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1016\/B978-0-444-52149-1.00063-X","volume-title":"The yeasts","author":"Kurtzman","year":"2011"},{"key":"2026081202533795100_toag165-B33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v082.i13","article-title":"lmerTest package: tests in linear mixed-effects models","volume":"82","author":"Kuznetsova","year":"2017","journal-title":"J. Stat. Soft"},{"key":"2026081202533795100_toag165-B34","first-page":"63","author":"Lang","year":"2015"},{"key":"2026081202533795100_toag165-B35","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1093\/ee\/nvy167","article-title":"Associations between Drosophila suzukii (Diptera: Drosophilidae) and fungi in Raspberries","volume":"48","author":"Lewis","year":"2019","journal-title":"Environ. Entomol"},{"key":"2026081202533795100_toag165-B36","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1111\/1365-2664.12688","article-title":"Enhanced yeast feeding following mating facilitates control of the invasive fruit pest Drosophila suzukii","volume":"54","author":"Mori","year":"2017","journal-title":"J. Appl. Ecol"},{"key":"2026081202533795100_toag165-B37","doi-asserted-by":"publisher","first-page":"2077","DOI":"10.1007\/s10340-025-01925-y","article-title":"Phagostimulant bait sprays improve control of spotted wing drosophila (Drosophila suzukii) in soft fruit","volume":"98","author":"Noble","year":"2025","journal-title":"J. Pest Sci. (2004)"},{"key":"2026081202533795100_toag165-B38","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1111\/jen.12917","article-title":"Minimizing insecticides for control of spotted wing drosophila (Drosophila suzukii) in soft fruit using bait sprays","volume":"145","author":"Noble","year":"2021","journal-title":"J. Applied Entomology"},{"key":"2026081202533795100_toag165-B39","doi-asserted-by":"publisher","first-page":"522","DOI":"10.3390\/horticulturae11050522","article-title":"Biocontrol in integrated pest management in fruit and vegetable field production","volume":"11","author":"Pobo\u017cniak","year":"2025","journal-title":"Horticulturae"},{"key":"2026081202533795100_toag165-B40","doi-asserted-by":"publisher","first-page":"bpz002","DOI":"10.1093\/biomethods\/bpz002","article-title":"Discrimination between Asian populations of the parasitoid wasp Ganaspis cf. brasiliensis using a simple MALDI-TOF MS-based method for use with insects","volume":"4","author":"Reeve","year":"2019","journal-title":"Biol. Methods Protoc"},{"key":"2026081202533795100_toag165-B41","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1002\/ps.6699","article-title":"Behavioral manipulation of Drosophila suzukii for pest control: high attraction to yeast enhances insecticide efficacy when applied on leaves","volume":"78","author":"Rehermann","year":"2022","journal-title":"Pest Manag. Sci"},{"key":"2026081202533795100_toag165-B42","doi-asserted-by":"publisher","first-page":"2003","DOI":"10.1093\/jee\/toaf170","article-title":"Establishment and parasitism levels of Ganaspis kimorum on Drosophila suzukii in Northeastern Italy: insights from a 4-yr release program","volume":"118","author":"Rossi-Stacconi","year":"2025","journal-title":"J. Econ. Entomol"},{"key":"2026081202533795100_toag165-B43","doi-asserted-by":"publisher","first-page":"63898","DOI":"10.3791\/63898","article-title":"Methods for rearing the parasitoid Ganaspis brasiliensis, a promising biological control agent for the invasive Drosophila suzukii","volume":"184","author":"Rossi-Stacconi","year":"2022","journal-title":"J. Vis. Exp"},{"key":"2026081202533795100_toag165-B44","doi-asserted-by":"publisher","first-page":"14059","DOI":"10.1038\/srep14059","article-title":"Volatile codes: correlation of olfactory signals and reception in Drosophila\u2013yeast chemical communication","volume":"5","author":"Scheidler","year":"2015","journal-title":"Sci. Rep"},{"key":"2026081202533795100_toag165-B45","doi-asserted-by":"publisher","first-page":"441","DOI":"10.3897\/jhr.97.118567","article-title":"Circumscription of the Ganaspis brasiliensis (Ihering, 1905) species complex (Hymenoptera, Figitidae), and the description of two new species parasitizing the spotted wing drosophila, Drosophila suzukii Matsumura, 1931 (Diptera, Drosophilidae)","volume":"97","author":"Sosa-Calvo","year":"2024","journal-title":"JHR"},{"key":"2026081202533795100_toag165-B46","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1007\/s10340-020-01266-y","article-title":"Yeast species affects feeding and fitness of Drosophila suzukii adults","volume":"93","author":"Spitaler","year":"2020","journal-title":"J. Pest Sci"},{"key":"2026081202533795100_toag165-B47","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.1002\/ps.6748","article-title":"Field and greenhouse application of an attract-and-kill formulation based on the yeast Hanseniaspora uvarum and the insecticide spinosad to control Drosophila suzukii in grapes","volume":"78","author":"Spitaler","year":"2022","journal-title":"Pest Manag. Sci"},{"key":"2026081202533795100_toag165-B48","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1093\/biomet\/64.1.156","article-title":"On distribution-free tests for equality of survival distributions","volume":"64","author":"Tarone","year":"1977","journal-title":"Biometrika"},{"key":"2026081202533795100_toag165-B49","doi-asserted-by":"publisher","author":"Therneau","year":"2024","DOI":"10.32614\/CRAN.package.survival"},{"key":"2026081202533795100_toag165-B50","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1016\/S0022-1910(01)00088-9","article-title":"A comparison of nectar- and honeydew sugars with respect to their utilization by the hymenopteran parasitoid Cotesia glomerata","volume":"47","author":"W\u00e4ckers","year":"2001","journal-title":"J. Insect Physiol"},{"key":"2026081202533795100_toag165-B51","doi-asserted-by":"publisher","author":"Wang","year":"2020","DOI":"10.1079\/PAVSNNR202015054"},{"key":"2026081202533795100_toag165-B52","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/978-3-319-24277-4_2","volume-title":"ggplot2: Elegant graphics for data analysis","author":"Wickham","year":"2016"}],"container-title":["Journal of Economic Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jee\/article-pdf\/119\/4\/2765\/68537019\/toag165.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jee\/article-pdf\/119\/4\/2765\/68537019\/toag165.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T06:53:43Z","timestamp":1786517623000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jee\/article\/119\/4\/2765\/8709198"}},"subtitle":[],"editor":[{"given":"Nik","family":"Wiman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,6,16]]},"references-count":52,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,6,16]]},"published-print":{"date-parts":[[2026,8,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jee\/toag165","relation":{},"ISSN":["0022-0493","1938-291X"],"issn-type":[{"value":"0022-0493","type":"print"},{"value":"1938-291X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,8]]},"published":{"date-parts":[[2026,6,16]]}}}