{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:43:45Z","timestamp":1786121025355,"version":"build-2736575974"},"reference-count":61,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004479","name":"Jiangxi Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["20252BAC200396"],"award-info":[{"award-number":["20252BAC200396"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Foundation Project of Jiangxi Provincial Educational Committee","award":["GJJ210443"],"award-info":[{"award-number":["GJJ210443"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Zeugodacus tau\u00a0Walker (Diptera: Tephritidae) is an invasive pest of global significance that inflicts substantial losses to the fruit and vegetable production sectors annually. Despite the availability of the Z. tau genome in public database, functional characterization of key genes remained limited. Vitellogenin (Vg) functions as a key precursor of yolk protein and exerts a pivotal part in the insect reproductive process. Here, we identified 3 Vg genes (Vg1, Vg2, and Vg3) based on genetic data from Z. tau. Analysis of amino acid sequences demonstrated that all ZtVgs contain conserved serine residues, a putative cleavage site, and putative N-linked glycosylation sites. Molecular phylogenetic reconstruction revealed that ZtVgs exhibited the closest evolutionary affinity to their homologs found in Zeugodacus cucurbitae and Bactrocera dorsalis. Spatiotemporal expression profiling indicated that all 3 ZtVgs exhibit pronounced expression in the female fat body. Treatments with the Methoprene and 20-hydroxyecdysone (20E) significantly upregulated ZtVgs expression 24\u2009h post application at a dose of 1\u2009\u03bcg. RNA interference (RNAi) bioassay revealed that the suppression of 3 ZtVgs delayed ovary development and impaired female fertility. Further microbiome analysis revealed that knockdown of the 3 Vg genes led to reduced abundance of bacterial taxa affiliated with the phylum Pseudomonadota and the genus Burkholderia in the ovary. These findings elucidate the important function of Vgs in ovary development and provide insights into the association between insect Vg genes and the ovarian bacteria, thereby establishing a groundwork for subsequent investigations on the molecular mechanisms underlying their interaction.<\/jats:p>","DOI":"10.1093\/jee\/toag239","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:42:58Z","timestamp":1784893378000},"source":"Crossref","is-referenced-by-count":0,"title":["RNAi-mediated knockdown of\n                    <i>vitellogenin<\/i>\n                    genes disrupts ovary development and alters ovarian bacterial composition in\n                    <i>Zeugodacus tau<\/i>\n                    (Diptera: Tephritidae)"],"prefix":"10.1093","author":[{"given":"Cuikang","family":"Xu","sequence":"first","affiliation":[{"name":"School of Agricultural Sciences, Jiangxi Agricultural University , Nanchang,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxiu","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Agricultural Sciences, Jiangxi Agricultural University , Nanchang,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhen","family":"Li","sequence":"additional","affiliation":[{"name":"School of Agricultural Sciences, Jiangxi Agricultural University , Nanchang,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9222-6173","authenticated-orcid":false,"given":"Weijun","family":"Li","sequence":"additional","affiliation":[{"name":"School of Agricultural Sciences, Jiangxi Agricultural University , Nanchang,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"2026080622041738400_toag239-B1","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/0022-1910(88)90035-2","article-title":"Interaction between juvenile hormone, 20-hydroxyecdysone, the corpus cardiacum-allatum complex, and the ovaries in regulating vitellogenin levels in the housefly, Musca domestica","volume":"34","author":"Adams","year":"1988","journal-title":"J. Insect. Physiol"},{"key":"2026080622041738400_toag239-B2","doi-asserted-by":"publisher","first-page":"16123","DOI":"10.1038\/s41598-017-16331-3","article-title":"Biofunctional analysis of Vitellogenin and Vitellogenin receptor in citrus red mites, Panonychus citri by RNA interference","volume":"7","author":"Ali","year":"2017","journal-title":"Sci. Rep"},{"key":"2026080622041738400_toag239-B3","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/s12861-019-0184-x","article-title":"Vitellogenins in the spider Parasteatoda tepidariorum\u2013expression profile and putative hormonal regulation of vitellogenesis","volume":"19","author":"Bednarek","year":"2019","journal-title":"BMC Dev. Biol"},{"key":"2026080622041738400_toag239-B4","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"2026080622041738400_toag239-B5","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1146\/annurev.ento.53.103106.093334","article-title":"Gonadal ecdysteroidogenesis in arthropoda: occurrence and regulation","volume":"54","author":"Brown","year":"2009","journal-title":"Annu. Rev. Entomol"},{"key":"2026080622041738400_toag239-B6","doi-asserted-by":"publisher","first-page":"452","DOI":"10.3390\/insects13050452","article-title":"Expression and role of vitellogenin genes in ovarian development of Zeugodacus cucurbitae","volume":"13","author":"Chen","year":"2022","journal-title":"Insects"},{"key":"2026080622041738400_toag239-B7","doi-asserted-by":"publisher","first-page":"8624","DOI":"10.1002\/ps.70167","article-title":"Rickettsia symbionts favor whitefly ovary development by promoting germ cell mitosis","volume":"81","author":"Chen","year":"2025","journal-title":"Pest Manag. Sci"},{"key":"2026080622041738400_toag239-B8","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/j.tibtech.2025.01.002","article-title":"dsRNAEngineer: a web-based tool of comprehensive dsRNA design for pest control","volume":"43","author":"Chen","year":"2025","journal-title":"Trends Biotechnol"},{"key":"2026080622041738400_toag239-B9","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: highly accurate OTU sequences from microbial amplicon reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Methods."},{"key":"2026080622041738400_toag239-B10","doi-asserted-by":"publisher","first-page":"e02997\u201302920","DOI":"10.1128\/mbio.02997-20","article-title":"The long and winding road for symbiont and yolk protein to host oocyte","volume":"12","author":"Fukatsu","year":"2021","journal-title":"mBio"},{"key":"2026080622041738400_toag239-B11","doi-asserted-by":"publisher","first-page":"175","DOI":"10.3390\/insects16020175","article-title":"Roles of vitellogenin and its receptor genes in female reproduction of the cigarette beetle, Lasioderma serricorne","volume":"16","author":"Guo","year":"2025","journal-title":"Insects"},{"key":"2026080622041738400_toag239-B12","doi-asserted-by":"publisher","first-page":"2016","DOI":"10.3389\/fmicb.2018.02016","article-title":"Vertical transmission of Wolbachia is associated with host vitellogenin in Laodelphax striatellus","volume":"9","author":"Guo","year":"2018","journal-title":"Front. Microbiol"},{"key":"2026080622041738400_toag239-B13","doi-asserted-by":"publisher","first-page":"103","DOI":"10.3389\/fphys.2014.00103","article-title":"Four-way regulation of mosquito yolk protein precursor genes by juvenile hormone-, ecdysone-, nutrient-, and insulin-like peptide signaling pathways","volume":"5","author":"Hansen","year":"2014","journal-title":"Front. Physiol"},{"key":"2026080622041738400_toag239-B14","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1007\/BF01840888","article-title":"An evolutionary model for the insect vitellins","volume":"18","author":"Harnish","year":"1982","journal-title":"J. Mol. Evol"},{"key":"2026080622041738400_toag239-B15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1673\/031.007.0101","article-title":"Mosquito vitellogenin genes: comparative sequence analysis, gene duplication, and the role of rare synonymous codon usage in regulating expression","volume":"7","author":"Isoe","year":"2007","journal-title":"J. Insect Sci"},{"key":"2026080622041738400_toag239-B16","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1093\/glycob\/cwh105","article-title":"Structural characterization of the N-glycan moiety and site of glycosylation in vitellogenin from the decapod crustacean Cherax quadricarinatus","volume":"14","author":"Khalaila","year":"2004","journal-title":"Glycobiology"},{"key":"2026080622041738400_toag239-B17","doi-asserted-by":"publisher","first-page":"S475","DOI":"10.33549\/physiolres.935221","article-title":"Unusual functions of Insect vitellogenins: minireview","volume":"72","author":"Kodr\u00edk","year":"2023","journal-title":"Physiol. Res"},{"key":"2026080622041738400_toag239-B18","doi-asserted-by":"publisher","first-page":"814","DOI":"10.1038\/nbt.2676","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences","volume":"31","author":"Langille","year":"2013","journal-title":"Nat. Biotechnol"},{"key":"2026080622041738400_toag239-B19","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.dci.2016.11.019","article-title":"Gut symbiotic bacteria stimulate insect growth and egg production by modulating hexamerin and vitellogenin gene expression","volume":"69","author":"Lee","year":"2017","journal-title":"Dev. Comp. Immunol"},{"key":"2026080622041738400_toag239-B20","doi-asserted-by":"publisher","first-page":"4146","DOI":"10.1002\/ps.70527","article-title":"Identification and evaluation of two testis-specific serine\/threonine kinase genes from multi-tissue transcriptomes as potential genetic targets of sterile insect technique in Zeugodacus tau","volume":"82","author":"Li","year":"2026","journal-title":"Pest Manag. Sci"},{"key":"2026080622041738400_toag239-B21","doi-asserted-by":"publisher","first-page":"149655","DOI":"10.1016\/j.gene.2025.149655","article-title":"Diversity of symbiotic microbes and their potential functions associated with multiple development stages of Zeugodacus tau (Walker)","volume":"965","author":"Li","year":"2025","journal-title":"Gene"},{"key":"2026080622041738400_toag239-B22","doi-asserted-by":"publisher","first-page":"101333","DOI":"10.1016\/j.cbd.2024.101333","article-title":"Comparative analyses of the transcriptome among three development stages of Zeugodacus tau larvae (Diptera: Tephritidae)","volume":"52","author":"Li","year":"2024","journal-title":"Comp. Biochem. Physiol. Part D Genomics Proteomics."},{"key":"2026080622041738400_toag239-B23","doi-asserted-by":"publisher","first-page":"2311","DOI":"10.1016\/S2095-3119(18)62103-3","article-title":"Molecular cloning, expression profiling and RNA interference of a vitellogenin gene from Harmonia axyridis (Coleoptera: Coccinellidae)","volume":"18","author":"Liang","year":"2019","journal-title":"J. Integr. Agric."},{"key":"2026080622041738400_toag239-B24","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1111\/1744-7917.12974","article-title":"Early embryonic transcriptomes of Zeugodacus tau provide insight into sex determination and differentiation genes","volume":"29","author":"Liu","year":"2022","journal-title":"Insect Sci"},{"key":"2026080622041738400_toag239-B25","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.cbpa.2018.12.004","article-title":"Molecular characterization and juvenile hormone-regulated transcription of the vitellogenin receptor in the cabbage beetle Colaphellus bowringi","volume":"229","author":"Liu","year":"2019","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol"},{"key":"2026080622041738400_toag239-B26","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2026080622041738400_toag239-B27","doi-asserted-by":"publisher","first-page":"e01142\u201301120","DOI":"10.1128\/mbio.01142-20","article-title":"Insect bacterial symbiont-mediated vitellogenin uptake into oocytes to support egg development","volume":"11","author":"Mao","year":"2020","journal-title":"mBio"},{"key":"2026080622041738400_toag239-B28","doi-asserted-by":"publisher","first-page":"101885","DOI":"10.1016\/j.jspr.2021.101885","article-title":"Functional analysis of vitellogenin and juvenile hormone-mediated regulation in a Psocoptera insect Liposcelis entomophila (Enderlein)","volume":"94","author":"Miao","year":"2021","journal-title":"J. Stored Prod. Res"},{"key":"2026080622041738400_toag239-B29","doi-asserted-by":"publisher","first-page":"2181","DOI":"10.1093\/molbev\/msu171","article-title":"Not only for egg yolk\u2014functional and evolutionary insights from expression, selection, and structural analyses of Formica Ant vitellogenins","volume":"31","author":"Morandin","year":"2014","journal-title":"Mol. Biol. Evol"},{"key":"2026080622041738400_toag239-B30","doi-asserted-by":"publisher","first-page":"e0153984","DOI":"10.1371\/journal.pone.0153984","article-title":"Suppression of bedbug\u2019s reproduction by RNA interference of vitellogenin","volume":"11","author":"Moriyama","year":"2016","journal-title":"PLoS One."},{"key":"2026080622041738400_toag239-B31","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1093\/aesa\/saw025","article-title":"Oocyte vitellogenesis triggers the entry of yeast-like symbionts into the oocyte of Brown Planthopper (Hemiptera: Delphacidae)","volume":"109","author":"Nan","year":"2016","journal-title":"Ann. Entomol. Soc. Am"},{"key":"2026080622041738400_toag239-B32","doi-asserted-by":"publisher","first-page":"2680","DOI":"10.1002\/ps.5071","article-title":"Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives","volume":"74","author":"Niu","year":"2018","journal-title":"Pest Manag. Sci"},{"key":"2026080622041738400_toag239-B33","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/j.ibmb.2010.03.006","article-title":"Juvenile hormone regulation of vitellogenin synthesis in the red flour beetle, Tribolium castaneum","volume":"40","author":"Parthasarathy","year":"2010","journal-title":"Insect Biochem. Mol. Biol"},{"key":"2026080622041738400_toag239-B34","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/S0022-1910(99)00111-0","article-title":"Inhibition of vitellogenin synthesis in Apis mellifera workers by a juvenile hormone analogue, pyriproxyfen","volume":"46","author":"Pinto","year":"2000","journal-title":"J. Insect Physiol"},{"key":"2026080622041738400_toag239-B35","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"2026080622041738400_toag239-B36","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1002\/arch.10107","article-title":"Regulation of juvenile hormone biosynthesis in Heliothis virescens by Manduca sexta allatotropin","volume":"54","author":"Rachinsky","year":"2003","journal-title":"Arch. Insect Biochem. Physiol"},{"key":"2026080622041738400_toag239-B37","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1146\/annurev-ento-020117-043258","article-title":"Regulatory pathways controlling female insect reproduction","volume":"63","author":"Roy","year":"2018","journal-title":"Annu. Rev. Entomol"},{"key":"2026080622041738400_toag239-B38","doi-asserted-by":"publisher","first-page":"e01577\u201301522","DOI":"10.1128\/spectrum.01577-22","article-title":"Interaction of Liberibacter solanacearum with host Psyllid vitellogenin and its association with autophagy","volume":"10","author":"Sarkar","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"2026080622041738400_toag239-B39","doi-asserted-by":"publisher","first-page":"e43","DOI":"10.4039\/tce.2022.29","article-title":"Primary structure and expression of vitellogenin A and vitellogenin B in the desert locust, Schistocerca gregaria (Orthoptera: Acrididae)","volume":"154","author":"Sayed Ahmed","year":"2022","journal-title":"Can. Entomol"},{"key":"2026080622041738400_toag239-B40","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1111\/imb.12366","article-title":"Vitellogenin and its receptor play essential roles in the development and reproduction of the brown citrus aphid, Aphis (Toxoptera) citricidus","volume":"27","author":"Shang","year":"2018","journal-title":"Insect Mol. Biol"},{"key":"2026080622041738400_toag239-B41","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1194\/jlr.R600028-JLR200","article-title":"Molecular diversity and evolution of the large lipid transfer protein superfamily","volume":"48","author":"Smolenaars","year":"2007","journal-title":"J. Lipid Res"},{"key":"2026080622041738400_toag239-B42","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/S0022-1910(99)00207-3","article-title":"Regulation of vitellogenesis in the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)","volume":"46","author":"Sorge","year":"2000","journal-title":"J. Insect Physiol"},{"key":"2026080622041738400_toag239-B43","doi-asserted-by":"publisher","first-page":"e02990\u201302922","DOI":"10.1128\/mbio.02990-22","article-title":"Vitellogenin facilitates associations between the whitefly and a bacteriocyte symbiont","volume":"14","author":"Sun","year":"2023","journal-title":"mBio"},{"key":"2026080622041738400_toag239-B44","doi-asserted-by":"publisher","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol. Biol. Evol"},{"key":"2026080622041738400_toag239-B45","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1038\/hdy.2012.34","article-title":"Genetic variation in the Yolk protein expression network of Drosophila melanogaster: sex-biased negative correlations with longevity","volume":"109","author":"Tarone","year":"2012","journal-title":"Heredity. (Edinb)"},{"key":"2026080622041738400_toag239-B46","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1002\/arch.1059","article-title":"Molecular evidence for two vitellogenin genes and processing of vitellogenins in the American cockroach, Periplaneta americana","volume":"48","author":"Tufail","year":"2001","journal-title":"Arch. Insect Biochem. Physiol"},{"key":"2026080622041738400_toag239-B47","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1016\/j.jinsphys.2008.08.007","article-title":"Molecular characteristics of insect vitellogenins","volume":"54","author":"Tufail","year":"2008","journal-title":"J. Insect Physiol"},{"key":"2026080622041738400_toag239-B48","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1002\/arch.21185","article-title":"Analysis of the vitellogenin gene of rice moth, Corcyra cephalonica stainton","volume":"87","author":"Veerana","year":"2014","journal-title":"Arch. Insect Biochem. Physiol"},{"key":"2026080622041738400_toag239-B49","doi-asserted-by":"publisher","first-page":"e00012\u201300021","DOI":"10.1128\/Spectrum.00012-21","article-title":"Global geographic diversity and distribution of the Myxobacteria","volume":"9","author":"Wang","year":"2021","journal-title":"Microbiol. Spectr."},{"key":"2026080622041738400_toag239-B50","doi-asserted-by":"publisher","first-page":"848","DOI":"10.1038\/s41597-023-02765-0","article-title":"Chromosome-level genome assembly of an agricultural pest Zeugodacus tau (Diptera: Tephritidae)","volume":"10","author":"Wang","year":"2023","journal-title":"Sci. Data."},{"key":"2026080622041738400_toag239-B51","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","article-title":"Jalview Version 2-a multiple sequence alignment editor and analysis workbench","volume":"25","author":"Waterhouse","year":"2009","journal-title":"Bioinformatics"},{"key":"2026080622041738400_toag239-B52","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1127\/entomologia\/2023\/1988","article-title":"RNAi-based pesticides: current knowledge and potential applications for integrated pest management","volume":"43","author":"Wei","year":"2023","journal-title":"Entomol Gen"},{"key":"2026080622041738400_toag239-B53","doi-asserted-by":"publisher","first-page":"593613","DOI":"10.3389\/fcell.2020.593613","article-title":"Regulatory mechanisms of vitellogenesis in insects","volume":"8","author":"Wu","year":"2021","journal-title":"Front. Cell Dev. Biol"},{"key":"2026080622041738400_toag239-B54","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/s40168-020-00799-9","article-title":"Rhizosphere protists are key determinants of plant health","volume":"8","author":"Xiong","year":"2020","journal-title":"Microbiome"},{"key":"2026080622041738400_toag239-B55","doi-asserted-by":"publisher","first-page":"151106","DOI":"10.1016\/j.ijbiomac.2026.151106","article-title":"Ovary-specific transcriptomes and the essential role of vitelline membrane protein 26Ab in embryonic desiccation resistance and female fertility of Zeugodacus tau","volume":"351","author":"Xu","year":"2026","journal-title":"Int. J. Biol. Macromol"},{"key":"2026080622041738400_toag239-B56","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1038\/s42003-025-09489-4","article-title":"Pathogen promotes insect ovarian development by inducing a dual role of host ATPSyn-\u03b2","volume":"9","author":"Yuan","year":"2026","journal-title":"Commun. Biol"},{"key":"2026080622041738400_toag239-B57","doi-asserted-by":"publisher","first-page":"482","DOI":"10.3389\/fphys.2018.00482","article-title":"Involvement of Met and Kr-h1 in JH-mediated reproduction of female Bactrocera dorsalis (Hendel)","volume":"9","author":"Yue","year":"2018","journal-title":"Front. Physiol"},{"key":"2026080622041738400_toag239-B58","doi-asserted-by":"publisher","first-page":"368","DOI":"10.3389\/fphys.2019.00368","article-title":"Identification and expression patterns of three vitellogenin genes and their roles in reproduction of the alligatorweed flea beetle Agasicles hygrophila (Coleoptera: Chrysomelidae)","volume":"10","author":"Zhang","year":"2019","journal-title":"Front. Physiol"},{"key":"2026080622041738400_toag239-B59","doi-asserted-by":"publisher","first-page":"e1010411","DOI":"10.1371\/journal.pgen.1010411","article-title":"miR-309a is a regulator of ovarian development in the oriental fruit fly Bactrocera dorsalis","volume":"18","author":"Zhang","year":"2022","journal-title":"PLoS Genet"},{"key":"2026080622041738400_toag239-B60","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.gene.2016.08.025","article-title":"Molecular characterization and expression of vitellogenin gene from Spodoptera exigua exposed to cadmium stress","volume":"593","author":"Zhao","year":"2016","journal-title":"Gene"},{"key":"2026080622041738400_toag239-B61","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1111\/1744-7917.12841","article-title":"Identification of G protein-coupled receptors required for vitellogenesis and egg development in an insect with panoistic ovary","volume":"28","author":"Zheng","year":"2021","journal-title":"Insect Sci"}],"container-title":["Journal of Economic Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jee\/advance-article-pdf\/doi\/10.1093\/jee\/toag239\/70513083\/toag239.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jee\/advance-article-pdf\/doi\/10.1093\/jee\/toag239\/70513083\/toag239.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T02:04:24Z","timestamp":1786068264000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jee\/advance-article\/doi\/10.1093\/jee\/toag239\/8753718"}},"subtitle":[],"editor":[{"given":"Amr","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,6]]},"references-count":61,"URL":"https:\/\/doi.org\/10.1093\/jee\/toag239","relation":{},"ISSN":["0022-0493","1938-291X"],"issn-type":[{"value":"0022-0493","type":"print"},{"value":"1938-291X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,6]]},"article-number":"toag239"}}