{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:10:57Z","timestamp":1790021457011,"version":"4.0.1"},"reference-count":140,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The sterile insect technique (SIT) has long been a cornerstone of area-wide integrated pest management programs, and the eradication of the New World screwworm Cochliomyia hominivorax (Coquerel) from North and Central America is widely regarded as a landmark success. However, its resurgence underscores the need to modernize SIT strategies against this devastating pest. The development of genetic sexing strains (GSS) is a revolutionary idea supporting SIT, enabling efficient large-scale separation and the exclusive release of sterile males. Despite GSSs having been shown to significantly enhance the cost-effectiveness and field efficacy of operational SIT campaigns, no such sexing systems have yet been developed for C. hominivorax. Given recent advances in insect genomics, we consider the development of a screwworm GSS both feasible and timely. In this forum, we critically evaluate the potential for translating decades of GSS research into C. hominivorax by leveraging the CRISPR\/Cas9 gene-editing technology. To make evidence accessible to a broader audience, we present a narrative overview covering the (i) genetics of GSSs, (ii) current approaches for precise engineering of analogous sexing systems, (iii) knowledge gaps and technical challenges in developing CRISPR-engineered GSSs in screwworm, and (iv) regulatory landscape governing genome-edited insects in the Americas in comparison to other contemporaneous biotechnologies. We argue that establishing a screwworm GSS would provide immediate benefits to existing management programs and hope this synthesis will inform and encourage future studies on this effort.<\/jats:p>","DOI":"10.1093\/jee\/toag283","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:47:16Z","timestamp":1790020036000},"source":"Crossref","is-referenced-by-count":0,"title":["Advancing the sterile insect technique for the New World screwworm fly: the case for CRISPR-engineered genetic sexing strains"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0159-4480","authenticated-orcid":false,"given":"Daniel F","family":"Paulo","sequence":"first","affiliation":[{"name":"The University of New Mexico Department of Biology, , Albuquerque, NM,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6853-9884","authenticated-orcid":false,"given":"Omar S","family":"Akbari","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego , La Jolla, CA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9511-5139","authenticated-orcid":false,"given":"Scott M","family":"Geib","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agriculture Research Service, Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center Tropical Pest Genetics and Molecular Biology Research Unit, , Hilo, HI,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"2026092115471028900_toag283-B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.treopn.2026.06.015","article-title":"Resurgence of the New World screwworm and the future of genetic biocontrol","author":"Akbari","year":"2026","journal-title":"Trends Open"},{"issue":":","key":"2026092115471028900_toag283-B2","doi-asserted-by":"publisher","first-page":"1579","DOI":"10.1038\/s41559-026-03157-4","article-title":"Renewed investment is needed as the New World screwworm returns to the USA","volume":"10","author":"Andrews","year":"2026","journal-title":"Nat. Ecol. 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