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We present a 681\u00a0Mb chromosome-level genome assembled from Oxford Nanopore, PacBio HiFi, Illumina, and Hi-C data, yielding nine chromosome-length scaffolds consistent with 2n\u2009=\u200918. The assembly exhibits strong intra-chromosomal Hi-C contact signals and a repeat content of 55.8%, dominated by LINEs. We annotated 29\u2009231 protein-coding genes, including 6316 transposable element\u2013associated loci, with BUSCO completeness scores of 91% (eukaryota_odb10) and 73% (metazoa_odb10). Macrosynteny is well conserved with Echinococcus granulosus and Hymenolepis microstoma, moderately conserved with the trematode Schistosoma mansoni, but largely disrupted in the free-living flatworm Schmidtea mediterranea, revealing lineage-specific genome reorganization among flatworms. To enable functional genetics, we identified candidate genomic safe harbours\u2014intergenic loci within transcriptionally active neighborhoods, separated from adjacent genes by 2\u201310\u00a0kb gene-free buffers, flanked by convergently oriented genes, and containing CRISPR\/Cas9 PAM motifs (NGG)\u2014and compiled housekeeping promoters for stable transgene expression. Together, these resources provide a reference framework for chromosome evolution and parasitic adaptation in Diphyllobothriidea and establish practical entry points for CRISPR-based functional studies in cestodes.<\/jats:p>","DOI":"10.1093\/jhered\/esaf104","type":"journal-article","created":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T12:27:54Z","timestamp":1767529674000},"page":"927-934","source":"Crossref","is-referenced-by-count":0,"title":["A chromosome-level genome of the lethal tapeworm\n                    <i>Sparganum proliferum<\/i>\n                    , with candidate genomic safe harbours for functional genetics"],"prefix":"10.1093","volume":"117","author":[{"given":"Atsushi","family":"Hikima","sequence":"first","affiliation":[{"name":"Laboratory of Parasite Systems Biology, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa , Chiba 277-8562 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