{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:40:31Z","timestamp":1790959231281,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study assesses a plant-based Soytone Medium as an alternative to the animal-derived standard MRS Broth for the cultivation of Lactobacillaceae. The application focuses on five isolates that have shown probiotic potential for bacterial vaginosis treatment. Cultivation was performed in 300\u00a0mL bench-scale bioreactors, monitored for cell density, pH, lactate production and glucose consumption. The media's carbon and nitrogen concentrations and costing were quantified.<\/jats:p>\n               <jats:p>Though the medium's carbon concentrations were identical, the Soytone Medium had a higher carbon-to-nitrogen ratio than MRS (8.1 vs 6.6). Four strains achieved higher cell densities and maximum specific growth rates in the Soytone Medium. The greatest benefit was shown for L. crispatus 70.6PA, which had a 45% higher final cell density. A cost analysis showed that the Soytone Medium was 44% cheaper than MRS Broth. It was thus confirmed that the proposed plant-based Soytone Medium is a viable and less expensive alternative for Lactobacillaceae cultures in which exposure to animal products was also avoided.<\/jats:p>","DOI":"10.1093\/jimb\/kuaf021","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T12:47:47Z","timestamp":1752497267000},"source":"Crossref","is-referenced-by-count":2,"title":["Beyond MRS Broth: A Soytone Medium towards affordable culturing of South African vaginal <i>Lactobacillaceae<\/i> isolates"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7897-1577","authenticated-orcid":false,"given":"Obakeng Luthando","family":"Jona","sequence":"first","affiliation":[{"name":"University of Cape Town Centre for Bioprocess Engineering Research (CeBER), Department of Chemical Engineering, , Cape Town ,","place":["South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6442-6017","authenticated-orcid":false,"given":"Marijke A","family":"Fagan-Endres","sequence":"additional","affiliation":[{"name":"University of Cape Town Centre for Bioprocess Engineering Research (CeBER), Department of Chemical Engineering, , Cape Town ,","place":["South Africa"]},{"name":"Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town , Cape Town ,","place":["South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3658-8638","authenticated-orcid":false,"given":"Anna-Ursula","family":"Happel","sequence":"additional","affiliation":[{"name":"Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town , Cape Town ,","place":["South Africa"]},{"name":"University of Cape Town Division of Immunology, Department of Pathology, , Cape Town ,","place":["South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5460-1977","authenticated-orcid":false,"given":"Brian","family":"Kullin","sequence":"additional","affiliation":[{"name":"Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town , Cape Town ,","place":["South Africa"]},{"name":"University of Cape Town Division of Medical Virology, Department of Pathology, , Cape Town ,","place":["South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3151-3514","authenticated-orcid":false,"given":"Jo-Ann S","family":"Passmore","sequence":"additional","affiliation":[{"name":"Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town , Cape Town ,","place":["South Africa"]},{"name":"University of Cape Town Division of Medical Virology, Department of Pathology, , Cape Town ,","place":["South Africa"]},{"name":"National Health Laboratory Service , Cape Town ,","place":["South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2268-3506","authenticated-orcid":false,"given":"Susan T L","family":"Harrison","sequence":"additional","affiliation":[{"name":"University of Cape Town Centre for Bioprocess Engineering Research (CeBER), Department of Chemical Engineering, , Cape Town ,","place":["South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,7,14]]},"container-title":["Journal of Industrial Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimb\/advance-article-pdf\/doi\/10.1093\/jimb\/kuaf021\/63747644\/kuaf021.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimb\/advance-article-pdf\/doi\/10.1093\/jimb\/kuaf021\/63747644\/kuaf021.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T12:47:47Z","timestamp":1752497267000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimb\/advance-article\/doi\/10.1093\/jimb\/kuaf021\/8199913"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,14]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/jimb\/kuaf021","relation":{},"ISSN":["1367-5435","1476-5535"],"issn-type":[{"value":"1367-5435","type":"print"},{"value":"1476-5535","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,14]]},"article-number":"kuaf021"}}