{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T16:42:17Z","timestamp":1778690537813,"version":"3.51.4"},"reference-count":37,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100022840","name":"Serum Institute of India Pvt. 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Further analysis identified galactose present either in tryptone (as lactose) or soy-phytone, has caused strong autoinduction and is surmised as an interfering factor for IPTG induction. Synergistic combination of soy-phytone and yeast extract under conditions of controlled growth and auto-induction gave a good correlation between the cell growth and expression of the fHbp\u2013PorA chimeric protein when evaluated using PBD and central composite design. Using response optimization conditions, an optimized media was attained. A shake flask study was performed to validate the optimized media, and later, a fed-batch fermentation at 10\u00a0L scale was established to prove the scalability, consistency, and product quality using the optimized media.<\/jats:p>\n                  <jats:p>One-sentence summary This study reports optimization of cell growth and expression conditions for a recombinant chimeric meningococcal protein in E. coli that assures its industrial scale production and suitability for preclinical and clinical studies as a vaccine component against Meningococcal B serogroup.<\/jats:p>","DOI":"10.1093\/jimb\/kuag001","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T12:40:12Z","timestamp":1767789612000},"source":"Crossref","is-referenced-by-count":1,"title":["Statistical approach for media optimization of fHbp\u2013PorA chimeric protein expression in\n                    <i>Escherichia coli<\/i>\n                    ; development of a promising Meningococcal B vaccine"],"prefix":"10.1093","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7098-8375","authenticated-orcid":false,"given":"Annamraju","family":"Aswini","sequence":"first","affiliation":[{"name":"Serum Institute of India Pvt. Ltd. , Hadapsar, Pune, Maharashtra, 411028 ,","place":["India"]},{"name":"Faculty of Medical and Health Sciences, Symbiosis School of Biological Sciences, Symbiosis International (Deemed University) , Lavale, Pune, Maharashtra, 412115 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annamraju D","family":"Sarma","sequence":"additional","affiliation":[{"name":"Serum Institute of India Pvt. Ltd. , Hadapsar, Pune, Maharashtra, 411028 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sambhaji S","family":"Pisal","sequence":"additional","affiliation":[{"name":"Serum Institute of India Pvt. Ltd. , Hadapsar, Pune, Maharashtra, 411028 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6846-319X","authenticated-orcid":false,"given":"Selvan","family":"Ravindran","sequence":"additional","affiliation":[{"name":"Faculty of Medical and Health Sciences, Symbiosis School of Biological Sciences, Symbiosis International (Deemed University) , Lavale, Pune, Maharashtra, 412115 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"2026020903364634400_bib1","doi-asserted-by":"publisher","first-page":"2567","DOI":"10.4314\/tjpr.v16i11.1","article-title":"Optimization of culture media for extracellular expression of streptokinase in Escherichia coli using response surface methodology in combination with Plackett-Burman design","volume":"16","author":"Aghaeepoor","year":"2017","journal-title":"Tropical Journal of 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