{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:29:10Z","timestamp":1787146150520,"version":"build-2736575974"},"reference-count":43,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":229,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20242BAB20253"],"award-info":[{"award-number":["20242BAB20253"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20243BCE51054"],"award-info":[{"award-number":["20243BCE51054"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["S2021GDQN2403"],"award-info":[{"award-number":["S2021GDQN2403"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20244BCE52262"],"award-info":[{"award-number":["20244BCE52262"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20243BCE51127"],"award-info":[{"award-number":["20243BCE51127"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"publisher","award":["32160579"],"award-info":[{"award-number":["32160579"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Program of Jiangxi Academy of Sciences","award":["2022YYB29"],"award-info":[{"award-number":["2022YYB29"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Filamentous fungi are important hosts for industrial enzyme production. Growing demand for \u03b1-amylase has increased reliance on food-derived carbon substrates, necessitating fungal strains that efficiently utilize nongrain biomass. In this study, Talaromyces pinophilus Y117 was metabolically engineered to produce \u03b1-amylase from lignocellulosic biomass. A strong cellobiohydrolase I gene (cbh1) promoter (Pcbh1Tru) was identified to drive expression. Multiple rounds of multilocus integration of the \u03b1-amylase gene were performed using homologous multicopy genomic sequences as recombination arms with a Cre\/loxP-based recyclable selection system, yielding the multicopy strain Tp4, which achieved 4124.5 U\/mL \u03b1-amylase activity in shake-flask fermentation with corncob powder as the sole carbon source. To minimize enzyme degradation, the protease gene 8538 was deleted using the Cre\/lox2272 system, generating Tp4\u0394p. This strain showed a 50% increase in shake-flask \u03b1-amylase activity (6208.4 U\/mL). In 3-L bioreactor cultivation, Tp4\u0394p exhibited excellent production performance, achieving 26\u2009712.2 U\/mL \u03b1-amylase activity. When corncob powder was used as the sole substrate, the cellulose and hemicellulose degradation rates reached 90.00% and 70.01%, respectively, and the enzyme yield reached 213\u2009697.5 U per gram of corncob powder. This engineered strain demonstrates strong potential for industrial applications. The synthesis\u2013degradation synergistic optimization strategy provides a practical approach for engineering filamentous fungal cell factories to produce enzymes directly from lignocellulosic biomass.<\/jats:p>\n                  <jats:p>One sentence summary Metabolic engineering of Talaromyces pinophilus through promoter optimization, multicopy integration, and protease deletion enables efficient \u03b1-amylase production from lignocellulosic biomass, achieving 26\u2009712 U\/mL in bioreactor fermentation.<\/jats:p>","DOI":"10.1093\/jimb\/kuag021","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T11:16:30Z","timestamp":1786965390000},"source":"Crossref","is-referenced-by-count":0,"title":["Multistrategy metabolic engineering of\n                    <i>Talaromyces pinophilus<\/i>\n                    for \u03b1-amylase production from lignocellulosic biomass"],"prefix":"10.1093","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6923-8493","authenticated-orcid":false,"given":"Jing","family":"Zeng","sequence":"first","affiliation":[{"name":"Institute of Bio-Manufacturing, Jiangxi Academy of Sciences , Jiangxi ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Bio-Manufacturing, Jiangxi Academy of Sciences , Jiangxi ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaiwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Bio-Manufacturing, Jiangxi Academy of Sciences , Jiangxi ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Bio-Manufacturing, Jiangxi Academy of Sciences , Jiangxi ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Yue","sequence":"additional","affiliation":[{"name":"Institute of Bio-Manufacturing, Jiangxi Academy of Sciences , Jiangxi ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Bio-Manufacturing, Jiangxi Academy of Sciences , Jiangxi ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhui","family":"Nie","sequence":"additional","affiliation":[{"name":"Institute of Bio-Manufacturing, Jiangxi Academy of Sciences , Jiangxi ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Bio-Manufacturing, Jiangxi Academy of Sciences , Jiangxi 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