{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T09:03:03Z","timestamp":1780995783298,"version":"3.54.1"},"reference-count":86,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Disease","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI163809"],"award-info":[{"award-number":["R21AI163809"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI177653"],"award-info":[{"award-number":["R01AI177653"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19AI135976"],"award-info":[{"award-number":["U19AI135976"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["75N93019C00070"],"award-info":[{"award-number":["75N93019C00070"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Research Service","award":["GM135096"],"award-info":[{"award-number":["GM135096"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Alveolar macrophages (AMs) are lung-resident myeloid cells and airway sentinels for inhaled pathogens and environmental particles. While AMs can be highly inflammatory in response to respiratory viruses, they do not mount proinflammatory responses to all airborne pathogens. For example, we previously showed that AMs fail to mount a robust proinflammatory response to Mycobacterium tuberculosis. Here, we address this discrepancy by investigating the capacity of murine AMs for direct innate immune sensing, using LPS as a model. Use of LPS-coated fluorescent beads enabled us to distinguish between directly exposed and bystander cells to measure transcriptional responses, by RNA-sequencing after cell sorting, and cytokine responses, by flow cytometry. We find that AMs have decreased proinflammatory responses to low-dose LPS compared to other macrophage types (bone marrow\u2013derived macrophages, peritoneal macrophages), as measured by TNF, IL-6, Ifnb, and Ifit3. The reduced response to low-dose LPS correlates with minimal TLR4 and CD14 surface expression, despite sufficient internal expression of TLR4. We also find that AMs do not produce IL-10 in response to a variety of stimuli due to low expression of the transcription factor c-Maf, while exogenous c-Maf expression restores IL-10 production in AMs. Lastly, we show that lack of IL-10 enables type I IFN enhancement of AM responses to LPS. Overall, we demonstrate AMs have a cell-intrinsic hyporesponsiveness to LPS, which makes them uniquely tolerant to low-dose exposure. Regulation of AM innate responses by distinct CD14, c-Maf, and IL-10 expression patterns has important implications for both respiratory infections and environmental airborne exposures.<\/jats:p>","DOI":"10.1093\/jimmun\/vkae029","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T23:43:38Z","timestamp":1741131818000},"page":"551-564","source":"Crossref","is-referenced-by-count":7,"title":["Absence of c-Maf and IL-10 enables type I IFN enhancement of innate responses to LPS in alveolar macrophages"],"prefix":"10.1093","volume":"214","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1905-8785","authenticated-orcid":false,"given":"Pamelia N","family":"Lim","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]},{"name":"Graduate Program in Molecular and Cellular Biology, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maritza M","family":"Cervantes","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0589-0423","authenticated-orcid":false,"given":"Linh K","family":"Pham","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]},{"name":"Graduate Program in Animal Biotechnology & Biomedical Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sydney R","family":"Doherty","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Tufts","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1376-7425","authenticated-orcid":false,"given":"Divya","family":"Dubey","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]},{"name":"Graduate Program in Molecular and Cellular Biology, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dat","family":"Mai","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute , Seattle, WA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4031-5499","authenticated-orcid":false,"given":"Alan","family":"Aderem","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute , Seattle, WA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5236-6130","authenticated-orcid":false,"given":"Alan H","family":"Diercks","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute , Seattle, WA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7484-1193","authenticated-orcid":false,"given":"Alissa C","family":"Rothchild","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,3,3]]},"reference":[{"key":"2025032923544174100_vkae029-B1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1165\/rcmb.2015-0146OC","article-title":"Flow cytometric analysis of myeloid cells in human blood, bronchoalveolar lavage, and lung tissues","volume":"54","author":"Yu","year":"2016","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2025032923544174100_vkae029-B2","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1165\/rcmb.2016-0122OC","article-title":"Diversity of human and macaque airway immune cells at baseline and during tuberculosis infection","volume":"55","author":"Silver","year":"2016","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2025032923544174100_vkae029-B3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/nature12902","article-title":"Sessile alveolar macrophages communicate with alveolar epithelium to modulate immunity","volume":"506","author":"Westphalen","year":"2014","journal-title":"Nature"},{"key":"2025032923544174100_vkae029-B4","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.cell.2020.08.020","article-title":"Patrolling alveolar macrophages conceal bacteria from the immune system to maintain homeostasis","volume":"183","author":"Neupane","year":"2020","journal-title":"Cell"},{"key":"2025032923544174100_vkae029-B5","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1038\/s41586-020-03148-w","article-title":"Circuits between infected macrophages and T cells in SARS-CoV-2 pneumonia","volume":"590","author":"Grant","year":"2021","journal-title":"Nature"},{"key":"2025032923544174100_vkae029-B6","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41586-022-04802-1","article-title":"Inflammasome activation in infected macrophages drives COVID-19 pathology","volume":"606","author":"Sefik","year":"2022","journal-title":"Nature"},{"key":"2025032923544174100_vkae029-B7","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1084\/jem.20140825","article-title":"Alveolar macrophage-derived type I interferons orchestrate innate immunity to RSV through recruitment of antiviral monocytes","volume":"212","author":"Goritzka","year":"2015","journal-title":"J Exp Med"},{"key":"2025032923544174100_vkae029-B8","doi-asserted-by":"crossref","first-page":"128","DOI":"10.4049\/jimmunol.2101135","article-title":"Type I IFN signaling is essential for preventing IFN-\u03b3 hyperproduction and subsequent deterioration of antibacterial immunity during post-influenza pneumococcal infection","volume":"209","author":"Palani","year":"2022","journal-title":"J. Immunol"},{"key":"2025032923544174100_vkae029-B9","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.4049\/jimmunol.1700210","article-title":"IL-1 signaling prevents alveolar macrophage depletion during influenza and streptococcus pneumoniae coinfection","volume":"200","author":"Bansal","year":"2018","journal-title":"J Immunol"},{"key":"2025032923544174100_vkae029-B10","doi-asserted-by":"crossref","first-page":"e29879","DOI":"10.1371\/journal.pone.0029879","article-title":"Innate immune response of human alveolar macrophages during influenza A infection","volume":"7","author":"Wang","year":"2012","journal-title":"PLoS One"},{"key":"2025032923544174100_vkae029-B11","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1136\/thoraxjnl-2017-210010","article-title":"Neutrophils drive alveolar macrophage IL-1\u03b2 release during respiratory viral infection","volume":"73","author":"Peir\u00f3","year":"2018","journal-title":"Thorax"},{"key":"2025032923544174100_vkae029-B12","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.immuni.2007.07.013","article-title":"Alveolar macrophages are the primary interferon-\u03b1 producer in pulmonary infection with RNA viruses","volume":"27","author":"Kumagai","year":"2007","journal-title":"Immunity"},{"key":"2025032923544174100_vkae029-B13","doi-asserted-by":"crossref","first-page":"e7259","DOI":"10.1371\/journal.pone.0007259","article-title":"Pseudomonas aeruginosa LPS or flagellin are sufficient to activate TLR-dependent signaling in murine alveolar macrophages and airway epithelial cells","volume":"4","author":"Raoust","year":"2009","journal-title":"PLoS One"},{"key":"2025032923544174100_vkae029-B14","doi-asserted-by":"crossref","first-page":"3030","DOI":"10.1002\/eji.200737532","article-title":"Critical role for Ipaf in Pseudomonas aeruginosa-induced caspase-1 activation","volume":"37","author":"Franchi","year":"2007","journal-title":"Eur J Immunol"},{"key":"2025032923544174100_vkae029-B15","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1128\/IAI.66.7.3164-3169.1998","article-title":"Role of alveolar macrophages in initiation and regulation of inflammation in pseudomonas aeruginosa pneumonia","volume":"66","author":"Kooguchi","year":"1998","journal-title":"Infect Immun"},{"key":"2025032923544174100_vkae029-B16","doi-asserted-by":"crossref","first-page":"7557","DOI":"10.1073\/pnas.1501289112","article-title":"IL-1R signaling enables bystander cells to overcome bacterial blockade of host protein synthesis","volume":"112","author":"Copenhaver","year":"2015","journal-title":"Proc Natl Acad Sci USA."},{"key":"2025032923544174100_vkae029-B17","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.chom.2018.08.001","article-title":"Alveolar macrophages provide an early mycobacterium tuberculosis niche and initiate dissemination","volume":"24","author":"Cohen","year":"2018","journal-title":"Cell Host Microbe"},{"key":"2025032923544174100_vkae029-B18","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.aaw6693","article-title":"Alveolar macrophages generate a noncanonical NRF2-driven transcriptional response to Mycobacterium tuberculosis in vivo","volume":"4","author":"Rothchild","year":"2019","journal-title":"Sci Immunol"},{"key":"2025032923544174100_vkae029-B19","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1016\/j.cell.2011.09.051","article-title":"CD14 controls the LPS-induced endocytosis of Toll-like receptor 4","volume":"147","author":"Zanoni","year":"2011","journal-title":"Cell"},{"key":"2025032923544174100_vkae029-B20","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/ni1569","article-title":"TRAM couples endocytosis of Toll-like receptor 4 to the induction of interferon-\u03b2","volume":"9","author":"Kagan","year":"2008","journal-title":"Nat Immunol"},{"key":"2025032923544174100_vkae029-B21","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1126\/science.1087262","article-title":"Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway","volume":"301","author":"Yamamoto","year":"2003","journal-title":"Science"},{"key":"2025032923544174100_vkae029-B22","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/ni1207","article-title":"CD14 is required for MyD88-independent LPS signaling","volume":"6","author":"Jiang","year":"2005","journal-title":"Nat. Immunol"},{"key":"2025032923544174100_vkae029-B23","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1086\/515176","article-title":"Lipopolysaccharide structure influences the macrophage response via CD14-independent and CD14-dependent pathways","volume":"28","author":"Gangloff","year":"1999","journal-title":"Clin Infect Dis"},{"key":"2025032923544174100_vkae029-B24","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S1074-7613(00)80254-X","article-title":"Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice","volume":"4","author":"Haziot","year":"1996","journal-title":"Immunity"},{"key":"2025032923544174100_vkae029-B25","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1165\/rcmb.2021-0190OC","article-title":"Murine ex vivo cultured alveolar macrophages provide a novel tool to study tissue-resident macrophage behavior and function","volume":"66","author":"Gorki","year":"2022","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2025032923544174100_vkae029-B26","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1093\/bioinformatics\/btq057","article-title":"Fast and SNP-tolerant detection of complex variants and splicing in short reads","volume":"26","author":"Wu","year":"2010","journal-title":"Bioinformatics"},{"key":"2025032923544174100_vkae029-B27","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"2025032923544174100_vkae029-B28","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.cels.2015.12.004","article-title":"The Molecular Signatures Database (MSigDB) hallmark gene set collection","volume":"1","author":"Liberzon","year":"2015","journal-title":"Cell Syst"},{"key":"2025032923544174100_vkae029-B29","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci USA."},{"key":"2025032923544174100_vkae029-B30","doi-asserted-by":"publisher","first-page":"4371","DOI":"10.21105\/joss.04371","article-title":"anndata: Access and store annotated datamatrices","volume":"9","author":"Virshup","year":"2024","journal-title":"JOSS"},{"key":"2025032923544174100_vkae029-B31","doi-asserted-by":"crossref","first-page":"ra59","DOI":"10.1126\/scisignal.aaa2155","article-title":"Analysis of single-cell cytokine secretion reveals a role for paracrine signaling in coordinating macrophage responses to TLR4 stimulation","volume":"8","author":"Xue","year":"2015","journal-title":"Sci Signal"},{"key":"2025032923544174100_vkae029-B32","doi-asserted-by":"crossref","first-page":"109728","DOI":"10.1016\/j.celrep.2021.109728","article-title":"Single-cell secretion analysis reveals a dual role for IL-10 in restraining and resolving the TLR4-induced inflammatory response","volume":"36","author":"Alexander","year":"2021","journal-title":"Cell Rep"},{"key":"2025032923544174100_vkae029-B33","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nri2711","article-title":"The regulation of IL-10 production by immune cells","volume":"10","author":"Saraiva","year":"2010","journal-title":"Nat Rev Immunol"},{"key":"2025032923544174100_vkae029-B34","doi-asserted-by":"crossref","first-page":"7551","DOI":"10.4049\/jimmunol.177.11.7551","article-title":"Macrophages and myeloid dendritic cells, but not plasmacytoid dendritic cells, produce IL-10 in response to MyD88- and TRIF-dependent TLR signals, and TLR-independent signals","volume":"177","author":"Boonstra","year":"2006","journal-title":"J Immunol"},{"key":"2025032923544174100_vkae029-B35","doi-asserted-by":"crossref","first-page":"6705","DOI":"10.4049\/jimmunol.178.11.6705","article-title":"Cutting edge: involvement of the type I IFN production and signaling pathway in lipopolysaccharide-induced IL-10 production","volume":"178","author":"Chang","year":"2007","journal-title":"J Immunol"},{"key":"2025032923544174100_vkae029-B36","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1038\/s41590-022-01146-w","article-title":"Long-term culture-expanded alveolar macrophages restore their full epigenetic identity after transfer in vivo","volume":"23","author":"Subramanian","year":"2022","journal-title":"Nat Immunol"},{"key":"2025032923544174100_vkae029-B37","doi-asserted-by":"publisher","first-page":"D886","DOI":"10.1093\/nar\/gkae1142","article-title":"CZ CELLxGENE Discover: a single-cell data platform for scalable exploration, analysis and modeling of aggregated data","volume":"53","author":"Abdulla","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"2025032923544174100_vkae029-B38","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1016\/j.immuni.2022.08.010","article-title":"Biology of lung macrophages in health and disease","volume":"55","author":"Aegerter","year":"2022","journal-title":"Immunity"},{"key":"2025032923544174100_vkae029-B39","doi-asserted-by":"crossref","first-page":"3320","DOI":"10.1038\/s41467-019-11147-3","article-title":"IFN-\u03b3 selectively suppresses a subset of TLR4-activated genes and enhancers to potentiate macrophage activation","volume":"10","author":"Kang","year":"2019","journal-title":"Nat Commun"},{"key":"2025032923544174100_vkae029-B40","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1128\/IAI.67.1.206-212.1999","article-title":"Gamma interferon augments macrophage activation by lipopolysaccharide by two distinct mechanisms, at the signal transduction level and via an autocrine mechanism involving tumor necrosis factor alpha and interleukin-1","volume":"67","author":"Held","year":"1999","journal-title":"Infect Immun"},{"key":"2025032923544174100_vkae029-B41","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.1182\/blood.V99.9.3427","article-title":"Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-\u03b3: a molecular basis for priming and synergism with bacterial lipopolysaccharide","volume":"99","author":"Bosisio","year":"2002","journal-title":"Blood"},{"key":"2025032923544174100_vkae029-B42","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/s41590-019-0582-z","article-title":"Transcriptomic and epigenetic mechanisms underlying myeloid diversity in the lung","volume":"21","author":"Sajti","year":"2020","journal-title":"Nat. Immunol"},{"key":"2025032923544174100_vkae029-B43","doi-asserted-by":"crossref","first-page":"e0159329","DOI":"10.1371\/journal.pone.0159329","article-title":"Full spectrum of LPS activation in alveolar macrophages of healthy volunteers by whole transcriptomic profiling","volume":"11","author":"Pinilla-Vera","year":"2016","journal-title":"PLoS One"},{"key":"2025032923544174100_vkae029-B44","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1165\/rcmb.2016-0028OC","article-title":"c-Jun is required for nuclear factor-\u03baB-dependent, LPS-stimulated fos-related antigen-1 transcription in alveolar macrophages","volume":"55","author":"Mishra","year":"2016","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2025032923544174100_vkae029-B45","doi-asserted-by":"crossref","first-page":"e126556","DOI":"10.1172\/jci.insight.126556","article-title":"Single cell RNA sequencing identifies unique inflammatory airspace macrophage subsets","volume":"4","author":"Mould","year":"2019","journal-title":"JCI Insight"},{"key":"2025032923544174100_vkae029-B46","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1126\/science.1698311","article-title":"CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein","volume":"249","author":"Wright","year":"1990","journal-title":"Science"},{"key":"2025032923544174100_vkae029-B47","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.imlet.2011.12.002","article-title":"Similarities and differences of innate immune responses elicited by smooth and rough LPS","volume":"142","author":"Zanoni","year":"2012","journal-title":"Immunol Lett"},{"key":"2025032923544174100_vkae029-B48","doi-asserted-by":"crossref","first-page":"e1000474","DOI":"10.1371\/journal.ppat.1000474","article-title":"MARCO, TLR2, and CD14 are required for macrophage cytokine responses to mycobacterial trehalose dimycolate and Mycobacterium tuberculosis","volume":"5","author":"Bowdish","year":"2009","journal-title":"PLoS Pathog"},{"key":"2025032923544174100_vkae029-B49","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.cellimm.2009.03.008","article-title":"TLR2 and its co-receptors determine responses of macrophages and dendritic cells to lipoproteins of Mycobacterium tuberculosis","volume":"258","author":"Drage","year":"2009","journal-title":"Cell Immunol"},{"key":"2025032923544174100_vkae029-B50","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.intimp.2009.10.002","article-title":"CD14 but not MD2 transmit signals from DAMP","volume":"10","author":"Chun","year":"2010","journal-title":"Int Immunopharmacol"},{"key":"2025032923544174100_vkae029-B51","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1189\/jlb.0510265","article-title":"Membrane CD14, but not soluble CD14, is used by exoenzyme S from P. aeruginosa to signal proinflammatory cytokine production","volume":"90","author":"Berenger","year":"2011","journal-title":"J Leukoc Biol"},{"key":"2025032923544174100_vkae029-B52","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1182\/blood-2006-06-032961","article-title":"Contribution of Toll-like receptors to the innate immune response to Gram-negative and Gram-positive bacteria","volume":"109","author":"Elson","year":"2007","journal-title":"Blood"},{"key":"2025032923544174100_vkae029-B53","doi-asserted-by":"crossref","first-page":"e1009781","DOI":"10.1371\/journal.ppat.1009781","article-title":"Human macrophages utilize a wide range of pathogen recognition receptors to recognize Legionella pneumophila, including Toll-Like Receptor 4 engaging Legionella lipopolysaccharide and the Toll-like Receptor 3 nucleic-acid sensor","volume":"17","author":"Grigoryeva","year":"2021","journal-title":"PLoS Pathog"},{"key":"2025032923544174100_vkae029-B54","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.4049\/jimmunol.1001665","article-title":"TLR 2 and CD14 mediate innate immunity and lung inflammation to staphylococcal panton\u2013valentine leukocidin in vivo","volume":"186","author":"Zivkovic","year":"2011","journal-title":"J Immunol"},{"key":"2025032923544174100_vkae029-B55","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1038\/80833","article-title":"Pattern recognition receptors TLR4 and CD14 mediate response to respiratory syncytial virus","volume":"1","author":"Kurt-Jones","year":"2000","journal-title":"Nat Immunol"},{"key":"2025032923544174100_vkae029-B56","doi-asserted-by":"crossref","first-page":"868","DOI":"10.3892\/mmr.2021.12508","article-title":"All-trans retinoic acid promotes macrophage phagocytosis and decreases inflammation via inhibiting CD14\/TLR4 in acute lung injury","volume":"24","author":"Li","year":"2021","journal-title":"Mol Med Rep"},{"key":"2025032923544174100_vkae029-B57","doi-asserted-by":"crossref","first-page":"438","DOI":"10.4049\/immunohorizons.2000112","article-title":"Prevention of acute lung injury by a novel CD14-inhibitory receptor activator of the NF-\u03baB ligand peptide in mice","volume":"5","author":"Ju","year":"2021","journal-title":"Immunohorizons"},{"key":"2025032923544174100_vkae029-B58","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1111\/j.1365-2567.2008.02840.x","article-title":"CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis","volume":"125","author":"Wieland","year":"2008","journal-title":"Immunology"},{"key":"2025032923544174100_vkae029-B59","doi-asserted-by":"crossref","first-page":"e10183","DOI":"10.1371\/journal.pone.0010183","article-title":"Role of CD14 in a mouse model of acute lung inflammation induced by different lipopolysaccharide chemotypes","volume":"5","author":"Anas","year":"2010","journal-title":"PLoS One"},{"key":"2025032923544174100_vkae029-B60","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1007\/s00011-022-01641-8","article-title":"CD14 signaling mediates lung immunopathology and mice mortality induced by Achromobacter xylosoxidans","volume":"71","author":"Elias-Oliveira","year":"2022","journal-title":"Inflamm Res"},{"key":"2025032923544174100_vkae029-B61","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1128\/IAI.71.1.61-67.2003","article-title":"Interaction of pulmonary surfactant protein C with CD14 and lipopolysaccharide","volume":"71","author":"Augusto","year":"2003","journal-title":"Infect Immun"},{"key":"2025032923544174100_vkae029-B62","doi-asserted-by":"crossref","first-page":"22442","DOI":"10.1074\/jbc.M001107200","article-title":"Surfactant proteins A and D bind CD14 by different mechanisms","volume":"275","author":"Sano","year":"2000","journal-title":"J Biol Chem"},{"key":"2025032923544174100_vkae029-B63","doi-asserted-by":"crossref","first-page":"387","DOI":"10.4049\/jimmunol.163.1.387","article-title":"Pulmonary surfactant protein A modulates the cellular response to smooth and rough lipopolysaccharides by interaction with CD14","volume":"163","author":"Sano","year":"1999","journal-title":"J Immunol."},{"key":"2025032923544174100_vkae029-B64","doi-asserted-by":"crossref","first-page":"35878","DOI":"10.1074\/jbc.M807268200","article-title":"Pulmonary surfactant protein D inhibits lipopolysaccharide (LPS)-induced inflammatory cell responses by altering LPS binding to its receptors*","volume":"283","author":"Yamazoe","year":"2008","journal-title":"J Biol Chem"},{"key":"2025032923544174100_vkae029-B65","doi-asserted-by":"crossref","first-page":"e00250","DOI":"10.1128\/iai.00250-21","article-title":"Surfactant protein A enhances the degradation of LPS-induced TLR4 in primary alveolar macrophages involving Rab7, \u03b2-arrestin2, and mTORC1","volume":"90","author":"Freundt","year":"2022","journal-title":"Infect Immun"},{"key":"2025032923544174100_vkae029-B66","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.immuni.2010.09.010","article-title":"The Rab11a GTPase controls toll-like receptor 4-induced activation of interferon regulatory factor-3 on phagosomes","volume":"33","author":"Husebye","year":"2010","journal-title":"Immunity"},{"key":"2025032923544174100_vkae029-B67","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.immuni.2010.10.003","article-title":"Recycling endosomes and TLR signaling\u2014the Rab11 GTPase leads the way","volume":"33","author":"Kagan","year":"2010","journal-title":"Immunity"},{"key":"2025032923544174100_vkae029-B68","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1074\/jbc.M113.499194","article-title":"The small GTPase Arf6 is essential for the Tram\/Trif pathway in TLR4 signaling","volume":"289","author":"Van Acker","year":"2014","journal-title":"J Biol Chem"},{"key":"2025032923544174100_vkae029-B69","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.cellimm.2011.12.007","article-title":"Inhibition of clathrin\/dynamin-dependent internalization interferes with LPS-mediated TRAM\u2013TRIF-dependent signaling pathway","volume":"274","author":"Wang","year":"2012","journal-title":"Cell Immunol"},{"key":"2025032923544174100_vkae029-B70","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1002\/jlb.67.4.545","article-title":"Lack of IL-10 synthesis by murine alveolar macrophages upon lipopolysaccharide exposure. Comparison with peritoneal macrophages","volume":"67","author":"Salez","year":"2000","journal-title":"J Leukoc Biol"},{"key":"2025032923544174100_vkae029-B71","doi-asserted-by":"crossref","first-page":"aad5510","DOI":"10.1126\/science.aad5510","article-title":"Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells","volume":"351","author":"Soucie","year":"2016","journal-title":"Science"},{"key":"2025032923544174100_vkae029-B72","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1126\/science.1176056","article-title":"MafB\/c-Maf deficiency enables self-renewal of differentiated functional macrophages","volume":"326","author":"Aziz","year":"2009","journal-title":"Science"},{"key":"2025032923544174100_vkae029-B73","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.4049\/jimmunol.1700397","article-title":"Aging impairs alveolar macrophage phagocytosis and increases influenza-induced mortality in mice","volume":"199","author":"Wong","year":"2017","journal-title":"J Immunol"},{"key":"2025032923544174100_vkae029-B74","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.4049\/jimmunol.1900495","article-title":"Identification of an increased alveolar macrophage subpopulation in old mice that displays unique inflammatory characteristics and is permissive to Mycobacterium tuberculosis infection","volume":"203","author":"Lafuse","year":"2019","journal-title":"J Immunol"},{"key":"2025032923544174100_vkae029-B75","doi-asserted-by":"crossref","first-page":"644","DOI":"10.4049\/jimmunol.1901211","article-title":"IL-10 impairs local immune response in lung granulomas and lymph nodes during early mycobacterium tuberculosis infection","volume":"204","author":"Wong","year":"2020","journal-title":"J Immunol"},{"key":"2025032923544174100_vkae029-B76","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.150060","article-title":"Early IL-10 promotes vasculature-associated CD4+ T cells unable to control Mycobacterium tuberculosis infection","volume":"6","author":"Ferreira","year":"2021","journal-title":"JCI Insight"},{"key":"2025032923544174100_vkae029-B77","doi-asserted-by":"crossref","first-page":"e23316","DOI":"10.1096\/fj.202301722R","article-title":"Lactate produced by alveolar type II cells suppresses inflammatory alveolar macrophages in acute lung injury","volume":"37","author":"Roy","year":"2023","journal-title":"FASEB J"},{"key":"2025032923544174100_vkae029-B78","doi-asserted-by":"crossref","first-page":"e20200887","DOI":"10.1084\/jem.20200887","article-title":"Type I interferon signaling mediates Mycobacterium tuberculosis\u2013induced macrophage death","volume":"218","author":"Zhang","year":"2020","journal-title":"J Exp Med"},{"key":"2025032923544174100_vkae029-B79","first-page":"226","article-title":"Type I interferon, induced by adenovirus or adenoviral vector infection, regulates the cytokine response to lipopolysaccharide in a macrophage type-specific manner","volume":"16","author":"Maler","year":"2024","journal-title":"J Innate Immun"},{"key":"2025032923544174100_vkae029-B80","first-page":"119","author":"Huang","year":"2018"},{"key":"2025032923544174100_vkae029-B81","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/s41590-023-01739-z","article-title":"BCG immunization induces CX3CR1hi effector memory T cells to provide cross-protection via IFN-\u03b3-mediated trained immunity","volume":"25","author":"Tran","year":"2024","journal-title":"Nat Immunol"},{"key":"2025032923544174100_vkae029-B82","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1016\/j.cell.2018.09.042","article-title":"Induction of autonomous memory alveolar macrophages requires T cell help and is critical to trained immunity","volume":"175","author":"Yao","year":"2018","journal-title":"Cell"},{"key":"2025032923544174100_vkae029-B83","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.cell.2017.12.031","article-title":"BCG educates hematopoietic stem cells to generate protective innate immunity against tuberculosis","volume":"172","author":"Kaufmann","year":"2018","journal-title":"Cell"},{"key":"2025032923544174100_vkae029-B84","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1016\/j.cell.2014.11.023","article-title":"Environment drives selection and function of enhancers controlling tissue-specific macrophage identities","volume":"159","author":"Gosselin","year":"2014","journal-title":"Cell"},{"key":"2025032923544174100_vkae029-B85","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1016\/j.cell.2014.11.018","article-title":"Tissue-resident macrophage enhancer landscapes are shaped by the local microenvironment","volume":"159","author":"Lavin","year":"2014","journal-title":"Cell"},{"key":"2025032923544174100_vkae029-B86","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/s41590-019-0352-y","article-title":"The lung environment controls alveolar macrophage metabolism and responsiveness in type 2 inflammation","volume":"20","author":"Svedberg","year":"2019","journal-title":"Nat Immunol"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/214\/3\/551\/62239742\/vkae029.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/214\/3\/551\/62239742\/vkae029.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T18:35:20Z","timestamp":1743446120000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/214\/3\/551\/8050799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,1]]},"references-count":86,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jimmun\/vkae029","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,3]]},"published":{"date-parts":[[2025,3,1]]}}}