{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T02:09:04Z","timestamp":1771985344239,"version":"3.50.1"},"reference-count":71,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI124692"],"award-info":[{"award-number":["R01 AI124692"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI151148"],"award-info":[{"award-number":["R01 AI151148"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32 AI060546"],"award-info":[{"award-number":["T32 AI060546"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institutes of Heath","award":["R01 AI151148"],"award-info":[{"award-number":["R01 AI151148"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>CD8+ T cells are key effectors in immune control of Trypanosoma cruzi infection. Within C57BL\/6 mice, the T. cruzi-specific CD8+ T cell response is largely comprised of T cells recognizing trans-sialidase (TS)- and mucin-encoded epitopes. Despite their immunodominance, these epitope-specific CD8+ T cells are entirely dispensable for immune control. In a screen for epitopes inducing \u201cprotective\u201d CD8+ T cells, we uncovered a high level of cross-reactivity within the TSKb20-specific CD8+ T cell response. This cross-reactivity was driven by the TSKb20 epitope itself and not the infection. TCR sequencing defined key characteristics of the TSKb20 repertoire including biased TRBV12-1\/12-2 and TRBJ2-1\/2-7 gene usage and a highly dominant CDR3\u03b2 motif. The dispensability of the TSKb20 response in the control of T. cruzi infection along with the broad reactivity of this T cell population prompted us to assess the relative effector capacity of TSKb20 T cells at the site of reinfection. Similar to other activated CD8+ T cells at the site, TSKb20-specific CD8+ T cells expressed transcriptional patterns associated with effector function, suggesting that TSKb20 T cells are capable and likely participants in parasite control. These results indicate that broad TCR reactivity does not compromise the ability of TSKb20-specific T cells to develop into phenotypically functional effectors. Additionally, the failure to identify individual parasite epitopes capable of driving a protective CD8+ T cell response challenges the paradigm that individual T. cruzi epitopes, including highly immunodominant ones, are critical to or exploitable for the potent recognition of T. cruzi-infected host cells and infection control.<\/jats:p>","DOI":"10.1093\/jimmun\/vkaf107","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T14:10:27Z","timestamp":1750947027000},"page":"2000-2014","source":"Crossref","is-referenced-by-count":2,"title":["Highly cross-reactive and competent effectors dominate the CD8+ T cell response in <i>Trypanosoma cruzi<\/i> infection"],"prefix":"10.1093","volume":"214","author":[{"given":"Molly E","family":"Bunkofske","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Georgia , Athens, GA,","place":["United States"]},{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia , Athens, GA,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradyot","family":"Dash","sequence":"additional","affiliation":[{"name":"Department of Immunology, St Jude Children's Research Hospital , Memphis, TN,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walid","family":"Awad","sequence":"additional","affiliation":[{"name":"Department of Immunology, St Jude Children's Research Hospital , Memphis, TN,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul G","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Immunology, St Jude Children's Research Hospital , Memphis, TN,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9589-5243","authenticated-orcid":false,"given":"Rick L","family":"Tarleton","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia , Athens, GA,","place":["United States"]},{"name":"Department of Cellular Biology, University of Georgia , Athens, GA,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,6,16]]},"reference":[{"key":"2025082903522505100_vkaf107-B1","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1016\/S0140-6736(10)60061-X","article-title":"Chagas disease","volume":"375","author":"Rassi","year":"2010","journal-title":"Lancet"},{"key":"2025082903522505100_vkaf107-B2","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1590\/0074-02760140440","article-title":"Reaching for the Holy Grail: insights from infection\/cure models on the prospects for vaccines for Trypanosoma cruzi infection","volume":"110","author":"Bustamante","year":"2015","journal-title":"Mem Inst Oswaldo 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