{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:17:13Z","timestamp":1791523033679,"version":"4.3.4"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Scientific Research Foundation of SUMHS","award":["SSF-23-15-002"],"award-info":[{"award-number":["SSF-23-15-002"]}]},{"name":"Key Medical Disciplines of Shanghai Municipal Health Commission","award":["2024ZDXK0015"],"award-info":[{"award-number":["2024ZDXK0015"]}]},{"name":"Key Medical Disciplines of Shanghai Municipal Health Commission","award":["2024ZDXK0045"],"award-info":[{"award-number":["2024ZDXK0045"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Colorectal cancer (CRC) progression is closely associated with the dynamic evolution of the tumor microenvironment. As a key component of the tumor microenvironment, tumor-associated neutrophils exhibit protumor properties and dynamically regulate malignant processes by secreting bioactive mediators that coordinate interactions among immune cells. RACGAP1, a cytoplasmic division protein, is highly expressed in various cancers. Here, we investigated the impact of RACGAP1 on CRC cells and the immune microenvironment. Bioinformatics analysis revealed that RACGAP1 was overexpressed in CRC patients. In vitro, Racgap1 silencing triggered G2\/M phase arrest and significantly inhibited CRC cell proliferation and induced CRC cell apoptosis. In vivo, Racgap1 knockdown impaired tumor growth in mouse CRC models. RNA sequencing analysis demonstrated that Racgap1 expression significantly influenced immune-related pathways. Further analyses and experimental validation showed that Racgap1 was negatively correlated with neutrophil infiltration in CRC. Chemokine array data indicated that chemokines upregulated following Racgap1 silencing affected neutrophil migration and chemotaxis. Overall, our study suggests that RACGAP1 regulates the CRC microenvironment by modulating tumor cell apoptosis and neutrophil infiltration, highlighting its potential as a biomarker and immunotherapeutic target in CRC.<\/jats:p>","DOI":"10.1093\/jimmun\/vkaf111","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T10:09:38Z","timestamp":1749722978000},"page":"2087-2097","source":"Crossref","is-referenced-by-count":3,"title":["RACGAP1 promotes tumor progression by influencing neutrophil recruitment and tumor cell proliferation in colorectal cancer"],"prefix":"10.1093","volume":"214","author":[{"given":"Hong-Tai","family":"Ma","sequence":"first","affiliation":[{"name":"Central Laboratory, Shanghai Fengxian District Central Hospital, School of Medicine, Anhui University of Science and Technology , Shanghai,","place":["China"]},{"name":"Shanghai University of Medicine and Health Sciences Affiliated Sixth People's Hospital South Campus , Shanghai,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Qi","family":"Yin","sequence":"additional","affiliation":[{"name":"Central Laboratory, Shanghai Fengxian District Central Hospital, School of Medicine, Anhui University of Science and Technology , Shanghai,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia-Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory, Shanghai Fengxian District Central Hospital, School of Medicine, Anhui University of Science and Technology , Shanghai,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University , Shanghai,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Jie","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Ying","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai University of Medicine and Health Sciences Affiliated Sixth People's Hospital South Campus , 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