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Success in these endeavors is enhanced by expansion of small numbers of input NK cells driven by viral antigens or homeostatic proliferation in immunodeficient hosts. In contrast, analysis of other NK-cell functions, including immunoregulation, are non-proliferative and require an intact immune system in recipient mice. We reveal poor persistence of conventional congenic (CD45.1) BoyJ NK cells following adoptive transfer in comparison to CRISPR-generated CD45.1+ (JAXBoy) NK cells. Reciprocal transfers between C57BL\/6 and JAXBoy mice substantially improve seeding and maintenance of donor NK cells. Using this system, we confirm that CXCR3 re-positions NK cells in the white pulp of the spleen after infection, which is vital for immunoregulation. Moreover, we discovered that the transcription factor ASCL2 is required for recruitment of NK cells into the spleen and white pulp. These results provide improved tools and novel insights into NK cell biology.<\/jats:p>","DOI":"10.1093\/jimmun\/vkaf122","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T19:19:44Z","timestamp":1753211984000},"page":"1917-1925","source":"Crossref","is-referenced-by-count":0,"title":["Refined cell transfer model reveals roles for\n                    <i>Ascl2<\/i>\n                    and\n                    <i>Cxcr3<\/i>\n                    in splenic localization of mouse NK cells during virus infection"],"prefix":"10.1093","volume":"214","author":[{"given":"Laura M","family":"Canaday","sequence":"first","affiliation":[{"name":"Immunology Graduate Program, University of Cincinnati College of Medicine , Cincinnati, OH,","place":["United States"]},{"name":"Division of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children\u2018s Hospital Medical Center , Cincinnati, OH,","place":["United 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Etiology, Cincinnati Children\u2018s Hospital Medical Center , Cincinnati, OH,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison","family":"Yan","sequence":"additional","affiliation":[{"name":"Division of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children\u2018s Hospital Medical Center , Cincinnati, OH,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8346-257X","authenticated-orcid":false,"given":"Megan","family":"Wilson","sequence":"additional","affiliation":[{"name":"Division of Human Genetics, Center for Autoimmune Genomics and Etiology, Cincinnati Children\u2018s Hospital Medical Center , Cincinnati, OH,","place":["United 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