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This can be modeled using mixed bone marrow chimeras, where autoreactive 564Igi B cells initiate autoimmunity that spreads to wild-type (WT) B cells. The mechanisms controlling the inclusion of new B cell clones into spontaneous germinal centers (GCs) remain unclear. Using CRISPR-Cas9, we generated 2 autoreactive B cell receptor knock-in strains, M05 and G55, based on B cell receptors from WT GC B cells in WT:564Igi chimeras. M05 and G55 mice lacked spontaneous GCs and overt autoantibody production, with receptor editing (\u03bb light chain expression) contributing to tolerance. However, autoreactivity was not purged from the B cell compartment since presence of 564Igi clone allowed M05 and G55 B cells to join GCs and produce autoantibodies. These findings reveal that GCs can override peripheral tolerance, recruiting previously silent autoreactive clones and facilitating diversification of autoantibodies.<\/jats:p>","DOI":"10.1093\/jimmun\/vkaf223","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T21:40:31Z","timestamp":1759354831000},"page":"3208-3217","source":"Crossref","is-referenced-by-count":3,"title":["Dynamic control of autoreactive B cells from spontaneous germinal centers"],"prefix":"10.1093","volume":"214","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2021-2938","authenticated-orcid":false,"given":"Kristine","family":"Oleinika","sequence":"first","affiliation":[{"name":"Program in Cellular and Molecular Medicine, Boston Children\u2019s Hospital, Harvard Medical School , Boston, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexia","family":"Correia Ferreira","sequence":"additional","affiliation":[{"name":"Program in 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States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usha","family":"Nair","sequence":"additional","affiliation":[{"name":"The Ragon Institute of Mass General, MIT, and Harvard , Cambridge, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1130-9463","authenticated-orcid":false,"given":"Facundo D","family":"Batista","sequence":"additional","affiliation":[{"name":"The Ragon Institute of Mass General, MIT, and Harvard , Cambridge, MA,","place":["United States"]},{"name":"Department of Biology, Massachusetts Institute of Technology , Cambridge, MA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3213-4295","authenticated-orcid":false,"given":"Michael C","family":"Carroll","sequence":"additional","affiliation":[{"name":"Program in Cellular and Molecular Medicine, Boston Children\u2019s Hospital, Harvard Medical School , 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