{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T22:54:47Z","timestamp":1777762487341,"version":"3.51.4"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400260"],"award-info":[{"award-number":["81400260"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanxi Fundamental Research Fund","award":["20210302123267"],"award-info":[{"award-number":["20210302123267"]}]},{"name":"Shanxi Fundamental Research Fund","award":["202403021221315"],"award-info":[{"award-number":["202403021221315"]}]},{"name":"Shanxi Provincial Health Commission \u201cFour batch\u201d Science and technology medical innovation plan","award":["2023XM023"],"award-info":[{"award-number":["2023XM023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ventricular arrhythmias are a primary cause of sudden cardiac death after acute myocardial infarction (AMI). Both \u03b21-adrenergic autoantibodies (\u03b21-AA) and nociceptin\/orphanin FQ (N\/OFQ) are implicated in AMI, but their potential interaction in ischemic arrhythmogenesis remains unclear. This study investigated the interplay between N\/OFQ and \u03b21-AA and the underlying mechanism. A rat model of AMI was established by ligating the left anterior descending coronary artery. Rats were divided into Sham, coronary artery occlusion (CAO), CAO+NOP receptor antagonist (C-24), CAO+\u03b21-AA+metoprolol, CAO+\u03b21-AA+endocytosis inhibitor (Pitstop), and CAO+\u03b21-AA groups. Hemodynamics and ventricular arrhythmias were recorded, and serum levels of N\/OFQ and \u03b21-AA were measured by ELISA. The expression and localization of \u03b21-adrenergic receptors (\u03b21-AR) on cardiomyocyte membranes were analyzed by Western blot and immunofluorescence. Serum levels of both N\/OFQ and \u03b21-AA were significantly elevated in the CAO group compared to sham. Treatment with the N\/OFQ receptor antagonist C-24 suppressed the ischemia-induced increase in \u03b21-AA. The CAO group exhibited a significantly higher incidence of ventricular arrhythmias (VEB, VT\/VF) compared to sham and C-24 groups. Administration of exogenous \u03b21-AA further aggravated arrhythmias, which was exacerbated by inhibiting \u03b21-AR internalization with Pitstop. Conversely, the \u03b21-AR blocker metoprolol exerted a protective effect. Western blot and immunofluorescence confirmed that \u03b21-AA and Pitstop treatment increased \u03b21-AR membrane expression, indicating inhibited receptor internalization. During acute myocardial ischemia, N\/OFQ is associated with elevated levels of \u03b21-AA, which may contribute to ventricular arrhythmias, potentially via inhibiting the internalization of \u03b21-AR.<\/jats:p>","DOI":"10.1093\/jimmun\/vkag072","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T14:29:48Z","timestamp":1777472988000},"source":"Crossref","is-referenced-by-count":0,"title":["The autoantibody \u03b21-AA contributes to N\/OFQ-exacerbated ventricular arrhythmias following acute myocardial ischemia"],"prefix":"10.1093","volume":"215","author":[{"given":"Xin-Yu","family":"Shang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, The Second Hospital of Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]},{"name":"College of Anesthesiology, Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Second Hospital of Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]},{"name":"College of Anesthesiology, Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling-Ge","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Second Hospital of Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]},{"name":"College of Anesthesiology, Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Second Hospital of Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]},{"name":"College of Anesthesiology, Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Anesthesiology, Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]},{"name":"Department of Anesthesiology, Linfen People\u2019s Hospital , Linfen, Shanxi,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3385-9789","authenticated-orcid":false,"given":"Yi","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Second Hospital of Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]},{"name":"College of Anesthesiology, Shanxi Medical University , Taiyuan, Shanxi,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"2026042910294130500_vkag072-B1","first-page":"521","article-title":"National Center for Cardiovascular Diseases, The Writing Committee of the Report on Cardiovascular Health and Diseases in China. 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