{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T15:10:43Z","timestamp":1788793843921,"version":"build-2803163510"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Canadian Blood Services Intramural Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Immune platelet transfusion refractoriness (iPTR) is a major complication in transfusion medicine. Although anti-HLA antibodies are recognized contributors, the mechanisms by which they drive platelet clearance remain incompletely defined. We examined sera from 18 patients with iPTR and found that anti-HLA antibodies induced uptake of human platelets expressing the cognate HLA-A2 target antigen and platelet particles derived from these platelets. These sera also mediated clearance of transgenic mouse platelets expressing human HLA-A2. Using THP-1\u2013CD16A macrophages, uptake of both platelets and platelet particles was mediated predominantly through Fc\u03b3RIIIa, with a partial contribution from Fc\u03b3RI and little to no role for Fc\u03b3RII. To test this pathway in vivo, we used a reductionist humanized murine model of iPTR in which HLA-A2 transgenic mouse platelets sensitized with human iPTR sera were transfused into Fc\u03b3R-humanized mice. In this model, Fc\u03b3RIII blockade with 17C02-albumin prevented platelet clearance under the conditions tested. These findings extend previous Fc\u03b3R studies in antibody-mediated platelet clearance to the alloimmune setting of iPTR and support further investigation of Fc\u03b3RIII-dependent pathways in antibody-mediated PTR.<\/jats:p>","DOI":"10.1093\/jimmun\/vkag195","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T17:02:51Z","timestamp":1785517371000},"source":"Crossref","is-referenced-by-count":0,"title":["Fc\u03b3RIII blockade prevents anti-HLA\u2013mediated platelet clearance in a humanized murine model of platelet transfusion refractoriness"],"prefix":"10.1093","volume":"215","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3042-8919","authenticated-orcid":false,"given":"Lazaro","family":"Gil Gonzalez","sequence":"first","affiliation":[{"name":"Keenan Research Centre for Biomedical Science, St. Michael\u2019s Hospital, Unity Health Toronto , Toronto, 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Oro","sequence":"additional","affiliation":[{"name":"Keenan Research Centre for Biomedical Science, St. Michael\u2019s Hospital, Unity Health Toronto , Toronto, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaima","family":"Tundidor Cabado","sequence":"additional","affiliation":[{"name":"Keenan Research Centre for Biomedical Science, St. Michael\u2019s Hospital, Unity Health Toronto , Toronto, ON,","place":["Canada"]},{"name":"Innovation, Canadian Blood Services , Ottawa, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoelys","family":"Cruz-Leal","sequence":"additional","affiliation":[{"name":"Keenan Research Centre for Biomedical Science, St. Michael\u2019s Hospital, Unity Health Toronto , Toronto, ON,","place":["Canada"]},{"name":"Innovation, Canadian Blood Services , Ottawa, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akash","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto , Toronto, ON,","place":["Canada"]},{"name":"Medical Affairs Division, Canadian Blood Services , Ottawa, ON,","place":["Canada"]},{"name":"Precision Diagnostics and Therapeutics Program, Sunnybrook Health Sciences Centre , Toronto, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Wabnitz","sequence":"additional","affiliation":[{"name":"Keenan Research Centre for Biomedical Science, St. Michael\u2019s Hospital, Unity Health Toronto , Toronto, ON,","place":["Canada"]},{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto , Toronto, ON,","place":["Canada"]},{"name":"Temerty Faculty of Medicine, University of Toronto , Toronto, ON,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich J","family":"Sachs","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine, and Haemostasis, Justus Liebig University , Giessen,","place":["Germany"]},{"name":"Department of Thrombosis and Haemostasis, Giessen University Hospital , Giessen,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rick","family":"Kapur","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Department Research, Amsterdam ,","place":["The Netherlands"]},{"name":"Amsterdam University Medical Center location University of Amsterdam, Landsteiner Laboratory, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan H","family":"Lazarus","sequence":"additional","affiliation":[{"name":"Keenan Research Centre for Biomedical Science, St. Michael\u2019s Hospital, Unity Health 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