{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:43:14Z","timestamp":1789486994489,"version":"build-2803163510"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"NCI Cancer Center Support","award":["#P30CA091842"],"award-info":[{"award-number":["#P30CA091842"]}]},{"name":"NIH NHGRI Institutional Training Grant in Genomic Science","award":["T32HG000045"],"award-info":[{"award-number":["T32HG000045"]}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>SWI\/SNF chromatin remodeling complexes regulate gene expression during development and differentiation. These complexes exist in distinct forms, including the polybromo-associated BAF (PBAF) complex, defined by the ARID2 subunit. ARID2 is frequently mutated in B cell leukemias, but its role in normal B cell development remains unknown. Using Mb1-Cre and CD19-Cre conditional mouse models, we show that Arid2 is required for normal peripheral B-cell maturation. Early deletion with Mb1-Cre caused marked reductions in splenic T1 and T2 transitional B cells, follicular B cells, and recirculating B cells, whereas later deletion with CD19-Cre produced a milder follicular B cell phenotype but a similar reduction in transitional B cells. Bone-marrow progenitor and immature B cell populations were largely preserved, suggesting that the developmental defect emerges during peripheral transitional maturation and follicular B cell differentiation. Transcriptomic profiling of isolated pro-B, pre-B, and follicular B cells revealed that Arid2 loss caused progressive, stage-specific transcriptional dysregulation, including altered AP-1-associated programs and reduced B cell receptor\u2013associated signaling in pre-B and follicular B cells. Functionally, mice with Mb1-Cre-mediated Arid2 deletion mice formed smaller germinal center B cell populations after immunization but maintained total and antigen-specific IgG responses and exhibited increased IgM responses. Together, these findings establish an important role for Arid2 in peripheral B cell maturation and germinal center responses.<\/jats:p>","DOI":"10.1093\/jimmun\/vkag247","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:39:28Z","timestamp":1789479568000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Arid2<\/i>\n                    regulates peripheral B cell maturation and germinal-center responses in vivo"],"prefix":"10.1093","volume":"215","author":[{"given":"Rachel L","family":"Paolini","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Oncology, Washington University School of Medicine , St. Louis, MO,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Oncology, Washington University School of Medicine , St. Louis, MO,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadra","family":"Marjai","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Oncology, Washington University School of Medicine , St. Louis, MO,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia R","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Oncology, Washington University School of Medicine , St. Louis, MO,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Oncology, Washington University School of Medicine , St. Louis, MO,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2196-8662","authenticated-orcid":false,"given":"George P","family":"Souroullas","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Oncology, Washington University School of Medicine , St. Louis, MO,","place":["United States"]},{"name":"Siteman Comprehensive Cancer Center, Washington University School of Medicine , St. Louis, Missouri,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"2026091509392087300_vkag247-B1","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1016\/j.jaci.2013.01.046","article-title":"B-cell biology and development","volume":"131","author":"Pieper","year":"2013","journal-title":"J Allergy Clin Immunol"},{"key":"2026091509392087300_vkag247-B2","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.coi.2008.03.014","article-title":"Peripheral B cell subsets","volume":"20","author":"Allman","year":"2008","journal-title":"Curr Opin Immunol"},{"key":"2026091509392087300_vkag247-B3","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1038\/nri3383","article-title":"Marginal zone B cells: virtues of innate-like antibody-producing lymphocytes","volume":"13","author":"Cerutti","year":"2013","journal-title":"Nat Rev Immunol"},{"key":"2026091509392087300_vkag247-B4","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/B978-0-12-385245-8.00005-4","volume-title":"Primer to the immune response","author":"Mak","year":"2014","edition":"2nd ed."},{"key":"2026091509392087300_vkag247-B5","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1038\/cmi.2017.35","article-title":"B cell regulation in cancer and anti-tumor immunity","volume":"14","author":"Sarvaria","year":"2017","journal-title":"Cell Mol Immunol"},{"key":"2026091509392087300_vkag247-B6","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1007\/s12016-015-8494-7","article-title":"Epigenetic control of B cell development and B-cell-related immune disorders","volume":"50","author":"Bao","year":"2016","journal-title":"Clinic Rev Allerg Immunol"},{"key":"2026091509392087300_vkag247-B7","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.4049\/jimmunol.1701470","article-title":"EZH2 represses the B cell transcriptional program and regulates antibody-secreting cell metabolism and antibody production","volume":"200","author":"Guo","year":"2018","journal-title":"J Immunol"},{"key":"2026091509392087300_vkag247-B8","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.ccr.2013.04.011","article-title":"EZH2 is required for germinal center formation and somatic EZH2 mutations promote lymphoid transformation","volume":"23","author":"B\u00e9guelin","year":"2013","journal-title":"Cancer Cell"},{"key":"2026091509392087300_vkag247-B9","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1038\/ni876","article-title":"Ezh2 controls B cell development through histone H3 methylation and Igh rearrangement","volume":"4","author":"Su","year":"2003","journal-title":"Nat Immunol"},{"key":"2026091509392087300_vkag247-B10","doi-asserted-by":"publisher","first-page":"3791","DOI":"10.4049\/jimmunol.1103390","article-title":"The SWI\/SNF-like BAF complex is essential for early B cell development","volume":"188","author":"Choi","year":"2012","journal-title":"J Immunol"},{"key":"2026091509392087300_vkag247-B11","doi-asserted-by":"publisher","first-page":"E718","DOI":"10.1073\/pnas.1418592112","article-title":"The SWI\/SNF chromatin remodeling complex regulates germinal center formation by repressing Blimp-1 expression","volume":"112","author":"Choi","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026091509392087300_vkag247-B12","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1146\/annurev-arplant-102820-093218","article-title":"The role and activity of SWI\/SNF chromatin remodelers","volume":"74","author":"Bieluszewski","year":"2023","journal-title":"Annu Rev Plant Biol"},{"key":"2026091509392087300_vkag247-B13","doi-asserted-by":"publisher","first-page":"e1500447-17","DOI":"10.1126\/sciadv.1500447","article-title":"Mammalian SWI\/SNF chromatin remodeling complexes and cancer: mechanistic insights gained from human genomics","volume":"1","author":"Kadoch","year":"2015","journal-title":"Sci Adv"},{"key":"2026091509392087300_vkag247-B14","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1016\/j.cell.2018.09.032","article-title":"Modular organization and assembly of SWI\/SNF family chromatin remodeling complexes","volume":"175","author":"Mashtalir","year":"2018","journal-title":"Cell"},{"key":"2026091509392087300_vkag247-B15","doi-asserted-by":"publisher","first-page":"7644","DOI":"10.1038\/s41467-022-34859-5","article-title":"Structure of nucleosome-bound human PBAF complex","volume":"13","author":"Wang","year":"2022","journal-title":"Nat Commun"},{"key":"2026091509392087300_vkag247-B16","doi-asserted-by":"publisher","first-page":"pl1","DOI":"10.1126\/scisignal.2004088","article-title":"Integrative analysis of complex cancer genomics and clinical profiles using the cBioPortal","volume":"6","author":"Gao","year":"2013","journal-title":"Sci Signal."},{"key":"2026091509392087300_vkag247-B17","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1158\/2159-8290.CD-12-0095","article-title":"The cBio cancer genomics portal: an open platform for exploring multidimensional cancer genomics data: figure 1","volume":"2","author":"Cerami","year":"2012","journal-title":"Cancer Discovery"},{"key":"2026091509392087300_vkag247-B18","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1186\/s12943-023-01736-8","article-title":"SWI\/SNF complexes in hematological malignancies: biological implications and therapeutic opportunities","volume":"22","author":"Andrades","year":"2023","journal-title":"Mol Cancer"},{"key":"2026091509392087300_vkag247-B19","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.1038\/s41588-022-01159-z","article-title":"The genomic landscape of pediatric acute lymphoblastic leukemia","volume":"54","author":"Brady","year":"2022","journal-title":"Nat Genet"},{"key":"2026091509392087300_vkag247-B20","doi-asserted-by":"publisher","first-page":"1704","DOI":"10.1038\/s41590-024-01920-y","article-title":"Arid1a-dependent canonical BAF complex suppresses inflammatory programs to drive efficient germinal center B cell responses","volume":"25","author":"Abraham","year":"2024","journal-title":"Nat Immunol"},{"key":"2026091509392087300_vkag247-B21","doi-asserted-by":"publisher","DOI":"10.1101\/2025.09.16.676393","article-title":"ARID1A and ARID1B safeguard B cell identity, prevent myeloid transformation and expose therapeutic vulnerabilities in lymphoma","author":"Lin","year":"2025","journal-title":"bioRxiv"},{"key":"2026091509392087300_vkag247-B22","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1016\/j.ccell.2024.02.010","article-title":"ARID1A orchestrates SWI\/SNF-mediated sequential binding of transcription factors with ARID1A loss driving pre-memory B cell fate and lymphomagenesis","volume":"42","author":"Barisic","year":"2024","journal-title":"Cancer Cell"},{"key":"2026091509392087300_vkag247-B23","doi-asserted-by":"publisher","first-page":"3047","DOI":"10.4049\/jimmunol.1002695","article-title":"Transcriptomes of the B and T lineages compared by multiplatform microarray profiling","volume":"186","author":"Painter","year":"2011","journal-title":"J Immunol"},{"key":"2026091509392087300_vkag247-B24","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1016\/j.stem.2007.10.003","article-title":"Hematopoietic fingerprints: an expression database of stem cells and their progeny","volume":"1","author":"Chambers","year":"2007","journal-title":"Cell Stem Cell"},{"key":"2026091509392087300_vkag247-B25","doi-asserted-by":"publisher","first-page":"D881","DOI":"10.1093\/nar\/gky1076","article-title":"BloodSpot: a database of healthy and malignant haematopoiesis updated with purified and single cell mRNA sequencing profiles","volume":"47","author":"Bagger","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2026091509392087300_vkag247-B26","doi-asserted-by":"publisher","first-page":"13789","DOI":"10.1073\/pnas.0605944103","article-title":"Testing gene function early in the B cell lineage in Mb1-Cre mice","volume":"103","author":"Hobeika","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026091509392087300_vkag247-B27","doi-asserted-by":"publisher","first-page":"1317","DOI":"10.1093\/nar\/25.6.1317","article-title":"B lymphocyte-specific, Cre-mediated mutagenesis in mice","volume":"25","author":"Rickert","year":"1997","journal-title":"Nucl Acids Res"},{"key":"2026091509392087300_vkag247-B28","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1038\/nn.2467","article-title":"A robust and high-throughput Cre reporting and characterization system for the whole mouse brain","volume":"13","author":"Madisen","year":"2010","journal-title":"Nat Neurosci"},{"key":"2026091509392087300_vkag247-B29","doi-asserted-by":"publisher","first-page":"2339","DOI":"10.1182\/blood-2006-05-021089","article-title":"Naive recirculating B cells mature simultaneously in the spleen and bone marrow","volume":"109","author":"Cariappa","year":"2007","journal-title":"Blood"},{"key":"2026091509392087300_vkag247-B30","doi-asserted-by":"publisher","first-page":"e25542","DOI":"10.1371\/journal.pone.0025542","article-title":"Toll-like receptor agonists synergize with CD40L to induce either proliferation or plasma cell differentiation of mouse B cells","volume":"6","author":"Boeglin","year":"2011","journal-title":"PLoS One"},{"key":"2026091509392087300_vkag247-B31","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1002\/(SICI)1521-4141(199803)28:03&lt;1040::AID-IMMU1040&gt;3.0.CO;2-9","article-title":"Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4","volume":"28","author":"Hasbold","year":"1998","journal-title":"Eur J Immunol"},{"key":"2026091509392087300_vkag247-B32","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1186\/1471-2105-10-161","article-title":"GAGE: generally applicable gene set enrichment for pathway analysis","volume":"10","author":"Luo","year":"2009","journal-title":"BMC Bioinformatics"},{"key":"2026091509392087300_vkag247-B33","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1097\/MOH.0000000000000511","article-title":"Toll-like receptor signaling in hematopoietic stem and progenitor cells","volume":"26","author":"Capitano","year":"2019","journal-title":"Curr Opin Hematol"},{"key":"2026091509392087300_vkag247-B34","doi-asserted-by":"publisher","first-page":"826554","DOI":"10.3389\/fcell.2021.826554","article-title":"Editorial: mechanisms of cell adhesion in hematopoietic stem cells","volume":"9","author":"Matsuura","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"2026091509392087300_vkag247-B35","doi-asserted-by":"publisher","first-page":"e20201545","DOI":"10.1084\/jem.20201545","article-title":"Inflammatory signaling regulates hematopoietic stem and progenitor cell development and homeostasis","volume":"218","author":"Collins","year":"2021","journal-title":"J Exp Med"},{"key":"2026091509392087300_vkag247-B36","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1038\/nri2656","article-title":"The follicular versus marginal zone B lymphocyte cell fate decision","volume":"9","author":"Pillai","year":"2009","journal-title":"Nat Rev Immunol"},{"key":"2026091509392087300_vkag247-B37","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/S1074-7613(01)00135-2","article-title":"The follicular versus marginal zone B lymphocyte cell fate decision is regulated by Aiolos, Btk, and CD21","volume":"14","author":"Cariappa","year":"2001","journal-title":"Immunity"},{"key":"2026091509392087300_vkag247-B38","doi-asserted-by":"publisher","first-page":"4477","DOI":"10.4049\/jimmunol.1502043","article-title":"STAT3 signaling in B cells is critical for germinal center maintenance and contributes to the pathogenesis of murine models of lupus","volume":"196","author":"Ding","year":"2016","journal-title":"J Immunol"},{"key":"2026091509392087300_vkag247-B39","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.it.2003.11.004","article-title":"Stressed-out B cells? Plasma-cell differentiation and the unfolded protein response","volume":"25","author":"Gass","year":"2004","journal-title":"Trends Immunol"},{"key":"2026091509392087300_vkag247-B40","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1016\/S1074-7613(00)80281-2","article-title":"Jak3 is associated with CD40 and is critical for CD40 induction of gene expression in B Cells","volume":"6","author":"Hanissian","year":"1997","journal-title":"Immunity"},{"key":"2026091509392087300_vkag247-B41","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1146\/annurev-immunol-092419-031130","article-title":"Age-associated B cells","volume":"38","author":"Cancro","year":"2020","journal-title":"Annu Rev Immunol"},{"key":"2026091509392087300_vkag247-B42","doi-asserted-by":"publisher","first-page":"1067","DOI":"10.1016\/j.molcel.2017.11.026","article-title":"AP-1 transcription factors and the SWI\/SNF complex mediate signal-dependent enhancer selection","volume":"68","author":"Vierbuchen","year":"2017","journal-title":"Mol Cell"},{"key":"2026091509392087300_vkag247-B43","doi-asserted-by":"publisher","first-page":"1497","DOI":"10.1073\/pnas.032665099","article-title":"Cooperation of multiple signaling pathways in CD40-regulated gene expression in B lymphocytes","volume":"99","author":"Dadgostar","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026091509392087300_vkag247-B44","doi-asserted-by":"publisher","first-page":"2326","DOI":"10.3390\/cancers13102326","article-title":"The role of AP-1 transcription factors in plasma cell biology and multiple myeloma pathophysiology","volume":"13","author":"Fan","year":"2021","journal-title":"Cancers (Basel)"},{"key":"2026091509392087300_vkag247-B45","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1038\/s41375-021-01505-w","article-title":"Differential roles of BAF and PBAF subunits, Arid1b and Arid2, in MLL-AF9 leukemogenesis","volume":"36","author":"Bluemn","year":"2022","journal-title":"Leukemia"},{"key":"2026091509392087300_vkag247-B46","doi-asserted-by":"publisher","first-page":"110637","DOI":"10.1016\/j.celrep.2022.110637","article-title":"Altered BAF occupancy and transcription factor dynamics in PBAF-deficient melanoma","volume":"39","author":"Carcamo","year":"2022","journal-title":"Cell Rep"},{"key":"2026091509392087300_vkag247-B47","doi-asserted-by":"publisher","first-page":"E2797","DOI":"10.1073\/pnas.1402159111","article-title":"Activation of Ras overcomes B-cell tolerance to promote differentiation of autoreactive B cells and production of autoantibodies","volume":"111","author":"Teodorovic","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026091509392087300_vkag247-B48","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1038\/s41571-020-0357-3","article-title":"The SWI\/SNF complex in cancer\u2013biology, biomarkers and therapy","volume":"17","author":"Mittal","year":"2020","journal-title":"Nat Rev Clin Oncol"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/215\/9\/vkag247\/71089270\/vkag247.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/215\/9\/vkag247\/71089270\/vkag247.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:39:29Z","timestamp":1789479569000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/doi\/10.1093\/jimmun\/vkag247\/8796043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,29]]},"references-count":48,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,8,29]]}},"URL":"https:\/\/doi.org\/10.1093\/jimmun\/vkag247","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,8,29]]},"article-number":"vkag247"}}