{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:30:01Z","timestamp":1788877801440,"version":"build-2803163510"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:00:00Z","timestamp":1702598400000},"content-version":"vor","delay-in-days":44,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The heat shock protein (HSP) gene families, present across prokaryotes to eukaryotes, play vital roles in growth, development, and heat resistance processes. While HSP proteins have been identified and characterized in various species, this study achieved the first genome-wide identification and characterization of HSP proteins in the Aedes aegypti genome. This study identified and assessed 80 potential HSP genes in Ae. aegypti. The phylogenetic relationships of HSP genes were investigated in Ae. aegypti, Anopheles stephensi, and Drosophila melanogaster. Additionally, the structural features, chromosomal locations, protein characteristics, 3D structure, protein\u2013protein interactions, and microsatellites associated with HSP proteins were examined in Ae. aegypti. The phylogenetic analysis of HSP gene families revealed distinct intra-group relationships for each HSP group. Each family exhibited relatively conserved genetic structures and motif components. In the expression analysis of growth and development, high expression was observed in certain HSP20 and HSP70 genes, while others exhibited low expression. Notably, sex-dependent expression differences were observed, particularly in HSP20 genes. These findings, the relationships, evolution, and modification of HSP gene families are illuminated by these comprehensive findings, and a better understanding of the mechanisms underlying growth, development, and heat resistance in vector organisms is facilitated.<\/jats:p>","DOI":"10.1093\/jisesa\/iead114","type":"journal-article","created":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T04:13:40Z","timestamp":1702700020000},"source":"Crossref","is-referenced-by-count":24,"title":["Genome-wide analysis and characterization of HSP gene families (HSP20, HSP40, HSP60, HSP70, HSP90) in the yellow fever mosquito <i>(Aedes aegypti)<\/i> (Diptera: Culicidae)"],"prefix":"10.1093","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2900-1755","authenticated-orcid":false,"given":"Murat","family":"Turan","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University , Erzurum , 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regulation in thermo-tolerant and thermo-susceptible A. mellifera L. subspecies in response to heat stress","volume-title":"Insects","author":"Alattal","year":"2023"},{"key":"2023121604082304800_CIT0005","first-page":"432","article-title":"Expression levels of heat-shock proteins in Apis mellifera jemenetica and Apis mellifera carnica foragers in the desert climate of Saudi Arabia","volume-title":"Insects","author":"Alghamdi","year":"2023"},{"key":"2023121604082304800_CIT0006","first-page":"e1000150","article-title":"The role of anorexia in resistance and tolerance to infections in Drosophila","volume-title":"PLoS Biol","author":"Ayres","year":"2009"},{"key":"2023121604082304800_CIT0007","first-page":"W369","article-title":"MEME: discovering and analyzing DNA and protein sequence motifs","volume-title":"Nucleic Acids Res","author":"Bailey","year":"2006"},{"key":"2023121604082304800_CIT0008","first-page":"327","article-title":"Insect behavioral restraint and adaptation strategies under 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roles","volume-title":"PLoS One","author":"Finetti","year":"2023"},{"key":"2023121604082304800_CIT0017","first-page":"e1001638","article-title":"Yellow fever in Africa: estimating the burden of disease and impact of mass vaccination from outbreak and serological data","volume-title":"PLoS Med","author":"Garske","year":"2014"},{"key":"2023121604082304800_CIT0018","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1385\/1-59259-890-0:571","article-title":"Protein identification and analysis tools on the ExPASy server","volume-title":"The proteomics protocols handbook","author":"Gasteiger","year":"2005"},{"key":"2023121604082304800_CIT0019","first-page":"535","article-title":"Hsp70 and small Hsps are the major heat shock protein members involved in midgut metamorphosis in the common cutworm, Spodoptera litura","volume-title":"Insect Mol Biol","author":"Gu","year":"2012"},{"key":"2023121604082304800_CIT0020","first-page":"175","article-title":"Impacts of 1.5\u00b0C Global Warming on 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stress","volume-title":"Acta Trop","author":"Sivan","year":"2017"},{"key":"2023121604082304800_CIT0048","first-page":"15223","article-title":"Interaction of the Hsp70 molecular chaperone, DnaK, with its cochaperone DnaJ","volume-title":"Proc Natl Acad Sci USA","author":"Suh","year":"1998"},{"key":"2023121604082304800_CIT0049","first-page":"5610","article-title":"Thermal-safety margins and the necessity of thermoregulatory behavior across latitude and elevation","volume-title":"Proc Natl Acad Sci USA","author":"Sunday","year":"2014"},{"key":"2023121604082304800_CIT0050","first-page":"D607","article-title":"STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets","volume-title":"Nucleic Acids Res","author":"Szklarczyk","year":"2019"},{"key":"2023121604082304800_CIT0051","first-page":"4876","article-title":"The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools","volume-title":"Nucleic Acids Res","author":"Thompson","year":"1997"},{"key":"2023121604082304800_CIT0052","first-page":"e0011218","article-title":"Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues","volume-title":"PLoS NeglTrop Dis","author":"Villegas","year":"2023"},{"key":"2023121604082304800_CIT0053","first-page":"77","article-title":"MapChart: software for the graphical presentation of linkage maps and QTLs","volume-title":"J Hered","author":"Voorrips","year":"2002"},{"key":"2023121604082304800_CIT0054","first-page":"101053","article-title":"Sex dependent transcriptome responses of the diamondback moth, Plutella xylostella L. to cold stress","volume-title":"Comp Biochem Physiol Part D Genomics Proteomics","author":"Wang","year":"2023"},{"key":"2023121604082304800_CIT0055","first-page":"20220011","article-title":"Assessing the role of family level variation and heat shock gene expression in the thermal stress response 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