{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:10:39Z","timestamp":1782133839581,"version":"3.54.5"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":2,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31960562"],"award-info":[{"award-number":["31960562"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guizhou Provincial Academy of Agricultural Sciences, China","award":["[2021] 45"],"award-info":[{"award-number":["[2021] 45"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The potential role of the juvenile hormone receptor gene (methoprene-tolerant, Met) in reproduction of Coccinella septempunctata L.\u00a0(Coleoptera: Coccinellidae)(Coleoptera: Coccinellidae), was investigated by cloning, analyzing expression profiles by quantitative real-time PCR, and via RNA interference (RNAi). CsMet encoded a 1518-bp open reading frames with a predicted protein product of 505 amino acids; the latter contained 2 Per-Arnt-Sim repeat profile at amino acid residues 30\u201383 and 102\u2013175. CsMet was expressed in different C. septempunctata larvae developmental stages and was most highly expressed in third instar. CsMet expression in female adults gradually increased from 20 to 30 d, and expression levels at 25 and 30 d were significantly higher than levels at 1\u201315 d. CsMet expression in 20-d-old male adults was significantly higher than in males aged 1\u201315 d. CsMet expression levels in fat body tissues of male and female adults were significantly higher than expression in the head, thorax, and reproductive system. At 5 and 10 d after CsMet-dsRNA injection, CsMet expression was significantly lower than the controls by 75.05% and 58.38%, respectively. Ovary development and vitellogenesis in C. septempunctata injected with CsMet-dsRNA were significantly delayed and fewer mature eggs were produced. This study provides valuable information for the large-scale rearing of C. septempunctata.<\/jats:p>","DOI":"10.1093\/jisesa\/ieae065","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T13:56:33Z","timestamp":1720014993000},"source":"Crossref","is-referenced-by-count":6,"title":["Cloning and functional analysis of the juvenile hormone receptor gene <i>CsMet<\/i> in <i>Coccinella septempunctata<\/i>"],"prefix":"10.1093","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1121-2722","authenticated-orcid":false,"given":"Ying","family":"Cheng","sequence":"first","affiliation":[{"name":"Insect Research Group,\u00a0Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences , Guiyang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Insect Research Group,\u00a0Institute of Plant Protection, Guizhou Provincial Academy of 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