{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:06:24Z","timestamp":1792785984625,"version":"4.5.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:00:00Z","timestamp":1776470400000},"content-version":"vor","delay-in-days":48,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Commissioned Research Project and the Anhui Provincial Graduate Innovation and Entrepreneurship Practice Project","award":["H202112"],"award-info":[{"award-number":["H202112"]}]},{"name":"Commissioned Research Project and the Anhui Provincial Graduate Innovation and Entrepreneurship Practice Project","award":["2022cxcysj180"],"award-info":[{"award-number":["2022cxcysj180"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dermatophagoides pteronyssinus (Trouessart)\u00a0and Dermatophagoides farinae (Hughes)\u00a0(Acariformes: Pyroglyphidae) are the prevalent kinds of house dust mites (HDMs). HDM is a common indoor pest, which mainly breeds in indoor dust and is an important allergen source causing a variety of allergic diseases. Effective detection of these HDMs is crucial in preventing the allergic diseases they cause. The objective of this study was to develop an innovative method for the rapid visualization of HDMs (D. pteronyssinus and D. farinae) using recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) in combination with CRISPR-Cas13a (RPA\u2013Cas13a\u2013LFD). To achieve heightened sensitivity in the detection of HDMs, Cas13a was incorporated into the RPA process and coupled with T7 transcripts. Based on this approach, a total of 2.23\u2013102 copies\/\u03bcl of HDM were detected within 35\u2009min (detection limit of 2.23 copies\/\u03bcl for D. farinae\u00a0and 39.7 copies\/\u03bcl for D. pteronyssinus). No cross-reactivity occurred with Aleuroglyphus ovatus (Troupeau) (Acariformes: Acaridae), Tyrophagus putrescentiae (Schrank) (Acariformes: Acaridae), Blomia tropicalis (van Bronswijk, de Cock &amp; Oshima) (Acariformes: Acaridae), Suidasia nesbitti (Hughes) (Acariformes: Acaridae), and Carpoglyphus lactis(Linnaeus) (Acariformes: Acaridae). The RPA\u2013Cas13a\u2013LFD methodology demonstrated high specificity and sensitivity in detecting HDM. Given its advantages, such as ease of operation, rapid detection, and time efficiency, it is well-suited for rapid field-based detection of HDMs, providing a new technical tool for detecting D. farinae\u00a0and D. pteronyssinus.<\/jats:p>","DOI":"10.1093\/jisesa\/ieag029","type":"journal-article","created":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T12:37:23Z","timestamp":1773491843000},"source":"Crossref","is-referenced-by-count":1,"title":["A novel rapid detection approach based on CRISPR-Cas13a for\n                    <i>Dermatophagoides pteronyssinus<\/i>\n                    and\n                    <i>Dermatophagoides farinae<\/i>\n                    \u00a0(Acariformes: Pyroglyphidae)"],"prefix":"10.1093","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8813-4061","authenticated-orcid":false,"given":"Siwen","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology, Wannan Medical University , Wuhu, Anhui,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Wannan Medical University , Wuhu, Anhui,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Auhui Provincial Engineering Research Centre for Molecular Detection and Diagnostics, Anhui Normal University , Wuhu,","place":["China"]},{"name":"Wuhu 3h Biotechnology Co., Ltd , Wuhu,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Wannan Medical University , Wuhu, Anhui,","place":["China"]},{"name":"Wuhu Mites Inspection and Control Engineering Technology Research Center , Wuhu,","place":["China"]},{"name":"Anhui Province Key Laboratory of Basic Research and Transformation of Age-related Diseases , Wuhu,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifan","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Wannan Medical University , Wuhu, Anhui,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5790-6261","authenticated-orcid":false,"given":"Renren","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Wannan Medical University , Wuhu, Anhui,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Wannan Medical University , Wuhu, Anhui,","place":["China"]},{"name":"Wuhu Mites Inspection and Control Engineering Technology Research Center , Wuhu,","place":["China"]},{"name":"Anhui Province Key Laboratory of Basic Research and Transformation of Age-related Diseases , Wuhu,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengli","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Wannan Medical University , Wuhu, Anhui,","place":["China"]},{"name":"Wuhu Mites Inspection and Control Engineering Technology Research Center , Wuhu,","place":["China"]},{"name":"Anhui Province Key Laboratory of Basic Research and Transformation of Age-related Diseases , Wuhu,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Chen","sequence":"additional","affiliation":[{"name":"Shandong Jiuxin Bioengineering Co., Ltd , Shandong,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dacun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong Jiuxin Bioengineering Co., Ltd , 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