{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T21:25:26Z","timestamp":1787261126995,"version":"build-2736575974"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":50,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-23-CE02-0009"],"award-info":[{"award-number":["ANR-23-CE02-0009"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018956","name":"LABEX TULIP","doi-asserted-by":"publisher","award":["ANR-10-LABX-0041"],"award-info":[{"award-number":["ANR-10-LABX-0041"]}],"id":[{"id":"10.13039\/100018956","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Respiratory metabolism is one of the key ecological traits of organisms. As such, it is studied by ecologists from a wide range of disciplines, from macroecological studies to evolutionary biology, with the global changes context making the need for its understanding more pressing. Unfortunately, properly measuring respiration is a time- and cost-intensive endeavor, and gathering large datasets can be prohibitive due to the difficulty of measuring it. Here, we describe a fluorescence-based high-throughput closed-system respirometry technique for terrestrial and aquatic invertebrates, able to measure respiration for up to 240 channels in parallel in vials of 2 or 4\u2009ml. We used a setup originally designed to continuously measure oxygen in laboratory cell cultures in liquid medium to measure arthropod respiration. Through a series of experiments, we demonstrate the reliability of the system to measure respiration of arthropods. Even very small arthropods were able to be measured, although Drosophila had to be measured in groups of 2 to 4. Further, the system\u2019s modularity makes it particularly fit for assessing full thermal performance curves of respiratory metabolism in a small amount of time. We provide helpful guidelines and R scripts to make use of this system to create thermal performance curves of insect metabolism.<\/jats:p>","DOI":"10.1093\/jisesa\/ieag076","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T11:27:32Z","timestamp":1783942052000},"source":"Crossref","is-referenced-by-count":0,"title":["Application of a high-throughput respirometry technique for assessing respiratory metabolism of terrestrial and aquatic invertebrates"],"prefix":"10.1093","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7764-0158","authenticated-orcid":false,"given":"Nicolas","family":"Mouret","sequence":"first","affiliation":[{"name":"Station d\u2019Ecologie Th\u00e9orique et Exp\u00e9rimentale, UAR 2029, CNRS , Moulis,","place":["France"]},{"name":"CNRS, Institut de Recherche pour le D\u00e9veloppement (IRD), Toulouse INP, Universit\u00e9 Toulouse 3 Paul Sabatier Centre de Recherche sur la Biodiversit\u00e9 et l'Environnement (CRBE), UMR 5300, , Toulouse,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3201-7862","authenticated-orcid":false,"given":"Laura","family":"Kouyoumdjian","sequence":"additional","affiliation":[{"name":"Station d\u2019Ecologie Th\u00e9orique et Exp\u00e9rimentale, UAR 2029, CNRS , Moulis,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9815-1621","authenticated-orcid":false,"given":"Chlo\u00e9","family":"Chabaud","sequence":"additional","affiliation":[{"name":"Station d\u2019Ecologie Th\u00e9orique et Exp\u00e9rimentale, UAR 2029, CNRS , Moulis,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3483-3716","authenticated-orcid":false,"given":"Maxime","family":"Stanislawek","sequence":"additional","affiliation":[{"name":"Station d\u2019Ecologie Th\u00e9orique et Exp\u00e9rimentale, UAR 2029, CNRS , Moulis,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9122-5348","authenticated-orcid":false,"given":"Emma","family":"Couranjou","sequence":"additional","affiliation":[{"name":"Station d\u2019Ecologie Th\u00e9orique et Exp\u00e9rimentale, UAR 2029, CNRS , Moulis,","place":["France"]},{"name":"CIRAD, Universit\u00e9 de la R\u00e9union UMR PVBMT, , Saint-Pierre, R\u00e9union,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pau","family":"Molina-Vila","sequence":"additional","affiliation":[{"name":"Station d\u2019Ecologie Th\u00e9orique et Exp\u00e9rimentale, UAR 2029, CNRS , Moulis,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5622-7907","authenticated-orcid":false,"given":"Elvire","family":"Bestion","sequence":"additional","affiliation":[{"name":"Station d\u2019Ecologie Th\u00e9orique et Exp\u00e9rimentale, UAR 2029, CNRS , Moulis,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"2026082016490066600_ieag076-B1","doi-asserted-by":"publisher","first-page":"e10179","DOI":"10.1002\/ece3.10179","article-title":"Thermal mismatches explain consumer\u2013resource dynamics in response to environmental warming","volume":"13","author":"\u00c1lvarez-Codesal","year":"2023","journal-title":"Ecol. 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Data and code for: \u201cApplication of a high-throughput respirometry technique for assessing respiratory metabolism of terrestrial and aquatic invertebrates\u201d. J. Insect Sci. Zenodo. 10.5281\/zenodo.21295638"},{"key":"2026082016490066600_ieag076-B30","doi-asserted-by":"publisher","first-page":"1867","DOI":"10.1111\/j.1420-9101.2009.01798.x","article-title":"Heritability of resting metabolic rate in a wild population of blue tits","volume":"22","author":"Nilsson","year":"2009","journal-title":"J. Evol. 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