{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:06:27Z","timestamp":1792785987250,"version":"4.5.0"},"reference-count":83,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T00:00:00Z","timestamp":1790985600000},"content-version":"vor","delay-in-days":32,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2014Next Generation EU","id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]},{"name":"MBM8K\u2014EndOFsymb\u2014Pest","award":["I53D23003320008"],"award-info":[{"award-number":["I53D23003320008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The olive fly Bactrocera oleae (Rossi) is one of the most destructive olive pests, threatening olive and olive oil production worldwide. Traditionally managed with synthetic insecticides, its control now requires alternative strategies due to regulatory restrictions. The disruption of the obligate association between the pest and its primary symbiont Candidatus Erwinia dacicola, Capuzzo et al. (2005) has been reported following the application of a Zn\u2013Cu\u2013citric acid bio-complex compound (Dentamet) to olives infested with newly laid eggs. In this study, the effects of Dentamet exposure on the composition and structure of the B. oleae bacterial community were evaluated by comparing flies from treated (Dentamet-treated [DT]) olives, with untreated (non-treated [NT]) olives under laboratory conditions, and olives collected from untreated orchards (wild-in-controlled-environment). Bacterial communities were characterized by 16S rRNA gene sequencing on an Illumina MiSeq platform, and quantitative Real-Time PCR (qPCR) was used to estimate the abundance of Ca. E. dacicola and 4 additional bacterial genera involved in detoxification and nutrient metabolism. Metabarcoding analyses revealed differences between DT and NT samples, including reduced bacterial diversity in DT larvae and a shift in the adult bacterial community structure supported by beta diversity analyses. qPCR analysis showed a lower abundance of Ca. E. dacicola in DT larvae (significant), pupae, and adults, alongside decreases in the 4 investigated bacterial genera. Overall, these findings suggest that Dentamet exposure is associated with changes not only in the primary symbiont but also in the broader bacterial community, with potential implications for key microbial interactions.<\/jats:p>","DOI":"10.1093\/jisesa\/ieag108","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:35:40Z","timestamp":1788521740000},"source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobial treatment of egg-infested olives is associated with perturbation and carry-over changes on bacterial communities in the olive fly\n                    <i>Bactrocera oleae<\/i>\n                    (Diptera, Tephritidae)"],"prefix":"10.1093","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9719-8275","authenticated-orcid":false,"given":"Laura","family":"Maretto","sequence":"first","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment\u2014DAFNAE, University of Padova , Legnaro,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4195-6614","authenticated-orcid":false,"given":"Ivana","family":"Carofano","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment\u2014DAFNAE, University of Padova , Legnaro,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Martinez-Sa\u00f1udo","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment\u2014DAFNAE, University of Padova , Legnaro,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1519-2044","authenticated-orcid":false,"given":"Marco","family":"Falasco","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment\u2014DAFNAE, University of Padova , Legnaro,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8307-9831","authenticated-orcid":false,"given":"Hannes","family":"Schuler","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural, Environmental and Food Sciences, Free University of Bozen-Bolzano , Bolzano-Bozen,","place":["Italy"]},{"name":"Competence Centre for Plant Health, Free University of Bozen-Bolzano , Bolzano-Bozen,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7363-431X","authenticated-orcid":false,"given":"Markus","family":"Riegler","sequence":"additional","affiliation":[{"name":"Hawkesbury Institute for the Environment, Western Sydney University , Penrith,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5341-7115","authenticated-orcid":false,"given":"Jennifer","family":"Morrow","sequence":"additional","affiliation":[{"name":"Hawkesbury Institute for the Environment, Western Sydney University , Penrith,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8459-893X","authenticated-orcid":false,"given":"Luca","family":"Mazzon","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and Environment\u2014DAFNAE, University of Padova , Legnaro,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,3]]},"reference":[{"key":"2026100311293411000_ieag108-B1","doi-asserted-by":"publisher","first-page":"e4473","DOI":"10.7717\/peerj.4473","article-title":"pcr: an R package for quality assessment, analysis and testing of qPCR data","volume":"6","author":"Ahmed","year":"2018","journal-title":"PeerJ."},{"key":"2026100311293411000_ieag108-B2","doi-asserted-by":"publisher","first-page":"211104","DOI":"10.1098\/rsos.211104","article-title":"Effects of symbiotic population impairment on microbiome composition and longevity of Bactrocera dorsalis","volume":"8","author":"Akami","year":"2021","journal-title":"R Soc. Open Sci"},{"key":"2026100311293411000_ieag108-B3","doi-asserted-by":"publisher","first-page":"2304","DOI":"10.3390\/microorganisms10112304","article-title":"Consumption of N2O by Flavobacterium azooxidireducens sp. nov. isolated from decomposing leaf litter of Phragmites australis (Cav.)","volume":"10","author":"Behrendt","year":"2022","journal-title":"Microorganisms"},{"key":"2026100311293411000_ieag108-B4","doi-asserted-by":"publisher","first-page":"919760","DOI":"10.3389\/fmicb.2022.919760","article-title":"Dynamics of the gut bacteriome during a laboratory adaptation process of the Mediterranean fruit fly, Ceratitis capitata","volume":"13","author":"Bel Mokhtar","year":"2022","journal-title":"Front. Microbiol"},{"key":"2026100311293411000_ieag108-B5","doi-asserted-by":"publisher","first-page":"2695","DOI":"10.1111\/jeb.12527","article-title":"Symbiotic bacteria enable olive flies (Bactrocera oleae) to exploit intractable sources of nitrogen","volume":"27","author":"Ben-Yosef","year":"2014","journal-title":"J. Evol. Biol"},{"key":"2026100311293411000_ieag108-B6","doi-asserted-by":"publisher","first-page":"150170","DOI":"10.1098\/rsos.150170","article-title":"Symbiotic bacteria enable olive fly larvae to overcome host defences","volume":"2","author":"Ben-Yosef","year":"2015","journal-title":"R Soc. Open Sci"},{"key":"2026100311293411000_ieag108-B7","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1111\/jen.12614","article-title":"Symbiosis interruption in the olive fly: effect of copper and propolis on Candidatus Erwinia dacicola","volume":"143","author":"Bigiotti","year":"2019","journal-title":"J. Appl. Entomol"},{"key":"2026100311293411000_ieag108-B8","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1111\/1744-7917.12835","article-title":"Bacterial symbiosis in Bactrocera oleae, an Achille\u2019s heel for its pest control","volume":"28","author":"Bigiotti","year":"2021","journal-title":"Insect Sci"},{"key":"2026100311293411000_ieag108-B9","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S1096-7176(03)00009-0","article-title":"Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways","volume":"5","author":"Blencke","year":"2003","journal-title":"Metab. Eng"},{"key":"2026100311293411000_ieag108-B10","doi-asserted-by":"publisher","first-page":"3778","DOI":"10.1093\/gbe\/evz258","article-title":"Functional genomics of a symbiotic community: shared traits in the olive fruit fly gut microbiota","volume":"12","author":"Blow","year":"2020","journal-title":"Genome Biol. Evol"},{"key":"2026100311293411000_ieag108-B11","doi-asserted-by":"publisher","first-page":"2244","DOI":"10.1021\/jf960922e","article-title":"Persistence of insecticide residues in olives and olive oil","volume":"45","author":"Cabras","year":"1997","journal-title":"J. Agric. Food Chem"},{"key":"2026100311293411000_ieag108-B12","unstructured":"Callahan BJ. (2024) Silva taxonomic training data formatted for DADA2 (version 138.2). Zenodo. 10.5281\/zenodo.14169026"},{"key":"2026100311293411000_ieag108-B13","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods."},{"key":"2026100311293411000_ieag108-B14","doi-asserted-by":"publisher","first-page":"868458","DOI":"10.3389\/fmicb.2022.868458","article-title":"Olive fruit fly symbiont population: impact of metamorphosis","volume":"13","author":"Campos","year":"2022","journal-title":"Front. Microbiol"},{"key":"2026100311293411000_ieag108-B15","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.1099\/ijs.0.63653-0","article-title":"'Candidatus Erwinia dacicola\u2019, a coevolved symbiotic bacterium of the olive fly Bactrocera oleae (Gmelin)","volume":"55","author":"Capuzzo","year":"2005","journal-title":"Int. J. Syst. Evol. Microbiol"},{"key":"2026100311293411000_ieag108-B16","doi-asserted-by":"publisher","first-page":"363","DOI":"10.3390\/insects13040363","article-title":"Oviposition deterrent activity of fungicides and low-risk substances for the integrated management of Bactrocera oleae (Diptera, Tephritidae)","volume":"13","author":"Checchia","year":"2022","journal-title":"Insects"},{"key":"2026100311293411000_ieag108-B17","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1007\/s00284-022-03157-0","article-title":"The role of Flavobacterium enshiense R6S-5-6 in the Wetland Ecosystem revealed by whole-genome analysis","volume":"80","author":"Choi","year":"2023","journal-title":"Curr. Microbiol"},{"key":"2026100311293411000_ieag108-B18","doi-asserted-by":"publisher","first-page":"4343","DOI":"10.1111\/1462-2920.14797","article-title":"Developmental stages and gut microenvironments influence gut microbiota dynamics in the invasive beetle Popillia japonica Newman (Coleoptera: Scarabaeidae)","volume":"21","author":"Chouaia","year":"2019","journal-title":"Environ. Microbiol"},{"key":"2026100311293411000_ieag108-B19","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1146\/annurev.ento.54.110807.090553","article-title":"Olive fruit fly: managing an ancient pest in modern times","volume":"55","author":"Daane","year":"2010","journal-title":"Annu. Rev. Entomol"},{"key":"2026100311293411000_ieag108-B20","doi-asserted-by":"publisher","first-page":"1890","DOI":"10.3389\/fmicb.2020.01890","article-title":"Comparative microbiomics of tephritid frugivorous pests (Diptera: Tephritidae) from the field: a tale of high variability across and within species","volume":"11","author":"De Cock","year":"2020","journal-title":"Front. Microbiol"},{"key":"2026100311293411000_ieag108-B21","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-3553-3_20","volume-title":"The biology of Acinetobacter","author":"Duine","year":"1991"},{"key":"2026100311293411000_ieag108-B22","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1007\/978-3-319-44409-3_5","volume-title":"Bacilli and agrobiotechnology","author":"Egamberdieva","year":"2016"},{"key":"2026100311293411000_ieag108-B23","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1007\/BF00384954","article-title":"Bacteria associated with four species of Dacus (Diptera: Tephritidae) and their role in the nutrition of the larvae","volume":"67","author":"Fitt","year":"1985","journal-title":"Oecologia"},{"key":"2026100311293411000_ieag108-B24","first-page":"269","article-title":"Reperti morfo-istologici sulle batteriosimbiosi del Dacus oleae Gmelin e di altri ditteri tripetidi, in natura e negli allevamenti su substrati artificiali","volume":"54","author":"Girolami","year":"1973","journal-title":"Redia"},{"key":"2026100311293411000_ieag108-B25","doi-asserted-by":"publisher","first-page":"4577","DOI":"10.1021\/jf800118t","article-title":"Effects of fly attack (Bactrocera oleae) on the phenolic profile and selected chemical parameters of olive oil","volume":"56","author":"Gomez-Caravaca","year":"2008","journal-title":"J. Agric. Food Chem"},{"key":"2026100311293411000_ieag108-B26","doi-asserted-by":"publisher","first-page":"868","DOI":"10.3390\/agronomy13030868","article-title":"The role of symbiont-targeted strategies in the management of pentatomidae and tephritidae pests under an integrated vision","volume":"13","author":"Gonella","year":"2023","journal-title":"Agronomy"},{"key":"2026100311293411000_ieag108-B27","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1127\/entomologia\/2022\/1707","article-title":"Symbiotic control of Halyomorpha halys using a microbial biopesticide","volume":"42","author":"Gonella","year":"2022","journal-title":"Entomologia"},{"key":"2026100311293411000_ieag108-B28","doi-asserted-by":"publisher","first-page":"547031","DOI":"10.3389\/fmicb.2020.547031","article-title":"Disruption of host-symbiont associations for the symbiotic control and management of pentatomid agricultural pest\u2014a review","volume":"11","author":"Gonella","year":"2020","journal-title":"Front. Microbiol"},{"key":"2026100311293411000_ieag108-B29","doi-asserted-by":"publisher","first-page":"E11951","DOI":"10.1073\/pnas.1809349115","article-title":"Microbiome interactions shape host fitness","volume":"115","author":"Gould","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026100311293411000_ieag108-B30","doi-asserted-by":"publisher","first-page":"698","DOI":"10.3390\/toxins14100698","article-title":"Corynebacterium sp. 2-TD mediated toxicity of 2-tridecanone to Helicoverpa armigera","volume":"14","author":"Gu","year":"2022","journal-title":"Toxins. (Basel)"},{"key":"2026100311293411000_ieag108-B31","doi-asserted-by":"publisher","first-page":"301","DOI":"10.21273\/HORTSCI.47.2.301","article-title":"Qualitative changes of olive oils obtained from fruits damaged by Bactrocera oleae (Rossi)","volume":"47","author":"Gucci","year":"2012","journal-title":"Horts."},{"key":"2026100311293411000_ieag108-B32","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.3389\/fmicb.2020.01357","article-title":"Dynamics of insect\u2013microbiome interaction influence host and microbial symbiont","volume":"11","author":"Gupta","year":"2020","journal-title":"Front. Microbiol"},{"key":"2026100311293411000_ieag108-B33","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1186\/s12866-019-1647-8","article-title":"Bacterial communities in the gut of wild and mass-reared Zeugodacus cucurbitae and Bactrocera dorsalis revealed by metagenomic sequencing","volume":"19","author":"Hadapad","year":"2019","journal-title":"BMC Microbiol"},{"key":"2026100311293411000_ieag108-B34","doi-asserted-by":"publisher","first-page":"20190068","DOI":"10.1098\/rstb.2019.0068","article-title":"Links between metamorphosis and symbiosis in holometabolous insects","volume":"374","author":"Hammer","year":"2019","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci"},{"key":"2026100311293411000_ieag108-B35","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1038\/ismej.2011.41","article-title":"Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea","volume":"5","author":"Herlemann","year":"2011","journal-title":"ISME J"},{"key":"2026100311293411000_ieag108-B36","doi-asserted-by":"publisher","first-page":"2533","DOI":"10.1007\/s00253-015-6439-y","article-title":"Acinetobacter species as model microorganisms in environmental microbiology","volume":"99","author":"Jung","year":"2015","journal-title":"Appl. Microbiol. Biotechnol"},{"key":"2026100311293411000_ieag108-B37","doi-asserted-by":"publisher","first-page":"e01003-23","DOI":"10.1128\/spectrum.01003-23","article-title":"Pangenome analysis of the genus Flavobacterium","volume":"11","author":"Kim","year":"2023","journal-title":"Microbiol. Spectr"},{"key":"2026100311293411000_ieag108-B38","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1111\/eea.12764","article-title":"The effects of geographic origin and antibiotic treatment on the gut symbiotic communities of Bactrocera oleae populations","volume":"167","author":"Koskinioti","year":"2019","journal-title":"Entomologia Exp. Applicata"},{"key":"2026100311293411000_ieag108-B39","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1111\/eea.12934","article-title":"Manipulation of insect gut microbiota towards the improvement of Bactrocera oleae artificial rearing","volume":"168","author":"Koskinioti","year":"2020","journal-title":"Entomologia Exp. Applicata"},{"key":"2026100311293411000_ieag108-B40","doi-asserted-by":"publisher","first-page":"3281","DOI":"10.1128\/AEM.02933-08","article-title":"Acetobacter tropicalis is a major symbiont of the olive fruit fly (Bactrocera oleae)","volume":"75","author":"Kounatidis","year":"2009","journal-title":"Appl. Environ. Microbiol"},{"key":"2026100311293411000_ieag108-B41","doi-asserted-by":"publisher","first-page":"14164","DOI":"10.1021\/acsomega.0c01907","article-title":"Simulation study for the degradation of some insecticides during different black table olive processes","volume":"5","author":"Kumral","year":"2020","journal-title":"ACS Omega"},{"key":"2026100311293411000_ieag108-B42","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1111\/mec.14920","article-title":"How quantitative is metabarcoding: a meta-analytical approach","volume":"28","author":"Lamb","year":"2019","journal-title":"Mol. Ecol"},{"key":"2026100311293411000_ieag108-B43","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1017\/S0007485321000031","article-title":"Stable inheritance of Candidatus Erwinia dacicola in olive fruit fly","volume":"111","author":"Livadaras","year":"2021","journal-title":"Bull. Entomol. Res"},{"key":"2026100311293411000_ieag108-B44","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2026100311293411000_ieag108-B45","doi-asserted-by":"publisher","first-page":"e0194131","DOI":"10.1371\/journal.pone.0194131","article-title":"Instar- and host-associated differentiation of bacterial communities in the Mediterranean fruit fly Ceratitis capitata","volume":"13","author":"Malacrin\u00f2","year":"2018","journal-title":"PLoS One"},{"key":"2026100311293411000_ieag108-B46","doi-asserted-by":"publisher","first-page":"3640","DOI":"10.1002\/ps.8068","article-title":"Copper nanoparticles interfere with insecticide sensitivity, fecundity and endosymbiont abundance in olive fruit fly Bactrocera oleae (Diptera: Tephritidae)","volume":"80","author":"Malandrakis","year":"2024","journal-title":"Pest Manag. Sci"},{"key":"2026100311293411000_ieag108-B47","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1016\/j.tifs.2015.04.009","article-title":"A review of Bactrocera oleae (Rossi) impact in olive products: from the tree to the table","volume":"44","author":"Malheiro","year":"2015","journal-title":"Trends Food Sci. Technol"},{"key":"2026100311293411000_ieag108-B48","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1186\/s40538-023-00450-3","article-title":"16S metabarcoding, total soil DNA content, and functional bacterial genes quantification to characterize soils under long-term organic and conventional farming systems","volume":"10","author":"Maretto","year":"2023","journal-title":"Chem. Biol. Technol. Agric"},{"key":"2026100311293411000_ieag108-B49","doi-asserted-by":"publisher","first-page":"13723","DOI":"10.1038\/s41598-023-40562-2","article-title":"Gut bacterial population and community dynamics following adult emergence in pest tephritid fruit flies","volume":"13","author":"Mason","year":"2023","journal-title":"Sci. Rep"},{"key":"2026100311293411000_ieag108-B50","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1016\/j.ympev.2010.02.016","article-title":"Phylogenetic relationships between flies of the Tephritinae subfamily (Diptera, Tephritidae) and their symbiotic bacteria","volume":"56","author":"Mazzon","year":"2010","journal-title":"Mol. Phylogenet. Evol"},{"key":"2026100311293411000_ieag108-B51","doi-asserted-by":"publisher","first-page":"e61217","DOI":"10.1371\/journal.pone.0061217","article-title":"phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data","volume":"8","author":"McMurdie","year":"2013","journal-title":"PLoS One."},{"key":"2026100311293411000_ieag108-B52","author":"M\u00fcller","year":"2023"},{"key":"2026100311293411000_ieag108-B53","doi-asserted-by":"publisher","first-page":"11737","DOI":"10.1038\/s41598-018-30179-1","article-title":"Sample size effects on the assessment of eukaryotic diversity and community structure in aquatic sediments using high-throughput sequencing","volume":"8","author":"Nascimento","year":"2018","journal-title":"Sci. Rep"},{"key":"2026100311293411000_ieag108-B54","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1111\/j.1439-0418.1978.tb01948.x","article-title":"The infestation of Dacus oleae (Gmel.) (Diptera: Tephritidae) at harvest time and its influence on yield and quality of olive oil in Crete","volume":"86","author":"Neuenschwander","year":"1978","journal-title":"Z. Angew. Entomol"},{"key":"2026100311293411000_ieag108-B55","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1186\/s40168-025-02169-9","article-title":"What lurks in the dark? An innovative framework for studying diverse wild insect microbiota","volume":"13","author":"Nowak","year":"2025","journal-title":"Microbiome"},{"key":"2026100311293411000_ieag108-B56","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.3390\/insects16111161","article-title":"Efficacy of attract-and-kill techniques in controlling Bactrocera oleae (Diptera: Tephritidae)","volume":"16","author":"Ortis","year":"2025","journal-title":"Insects"},{"key":"2026100311293411000_ieag108-B57","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.chemolab.2015.02.019","article-title":"zCompositions\u2014R package for multivariate imputation of left-censored data under a compositional approach","volume":"143","author":"Palarea-Albaladejo","year":"2015","journal-title":"Chemom. Intell. Lab. Syst"},{"key":"2026100311293411000_ieag108-B58","doi-asserted-by":"publisher","first-page":"e46984","DOI":"10.1371\/journal.pone.0046984","article-title":"The success of Acinetobacter species: genetic, metabolic and virulence attributes","volume":"7","author":"Peleg","year":"2012","journal-title":"PLoS One."},{"key":"2026100311293411000_ieag108-B59","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1127\/entomologia\/2023\/1916","article-title":"Impairing the development of an olive fly pest by targeting its symbiotic bacteria in egg-infested fruits","volume":"43","author":"Perin","year":"2023","journal-title":"Entomologia. (Pavia)"},{"key":"2026100311293411000_ieag108-B60","doi-asserted-by":"publisher","first-page":"e0292043","DOI":"10.1371\/journal.pone.0292043","article-title":"Aedes albopictus microbiota: differences between wild and mass-reared immatures do not suggest negative impacts from a diet based on black soldier fly larvae and fish food","volume":"18","author":"Polidori","year":"2023","journal-title":"PLoS One."},{"key":"2026100311293411000_ieag108-B61","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1127\/entomologia\/2023\/2224","article-title":"The intrinsic diversity of Nezara viridula gut symbionts affects the host fitness decline promoted by primary symbiont elimination","volume":"43","author":"Prieto","year":"2023","journal-title":"Entomologia"},{"key":"2026100311293411000_ieag108-B62","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1128\/jb.69.3.307-309.1955","article-title":"The flavobacteria: II. Utilization of nitrogen compounds","volume":"69","author":"Prince","year":"1955","journal-title":"J. Bacteriol"},{"key":"2026100311293411000_ieag108-B63","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"2026100311293411000_ieag108-B64","volume-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2024"},{"key":"2026100311293411000_ieag108-B65","doi-asserted-by":"publisher","first-page":"67","DOI":"10.11646\/zootaxa.1714.1.7","article-title":"On the senior authorship of Musca oleae (Diptera: Tephritidae)","volume":"1714","author":"Raspi","year":"2008","journal-title":"Zootaxa"},{"key":"2026100311293411000_ieag108-B66","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1017\/S0007485319000853","article-title":"Tephritidae fruit fly gut microbiome diversity, function and potential for applications","volume":"110","author":"Raza","year":"2020","journal-title":"Bull. Entomol. Res"},{"key":"2026100311293411000_ieag108-B67","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1111\/jam.14506","article-title":"Bacillus species in soil as a natural resource for plant health and nutrition","volume":"128","author":"Saxena","year":"2020","journal-title":"J. Appl. Microbiol"},{"key":"2026100311293411000_ieag108-B68","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1016\/j.csbj.2021.12.019","article-title":"The mosquito microbiome includes habitat-specific but rare symbionts","volume":"20","author":"Schrieke","year":"2022","journal-title":"Comput. Struct. Biotechnol. J"},{"key":"2026100311293411000_ieag108-B69","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1128\/jb.169.1.131-136.1987","article-title":"Discovery of a cutinase-producing Pseudomonas sp. cohabiting with a nitrogen-fixing Corynebacterium sp","volume":"169","author":"Sebastian","year":"1987","journal-title":"J. Bacteriol"},{"key":"2026100311293411000_ieag108-B70","doi-asserted-by":"publisher","first-page":"103793","DOI":"10.1016\/j.ibmb.2022.103793","article-title":"Dynamic interactions between the symbiont Candidatus Erwinia dacicola and its olive fruit fly host Bactrocera oleae","volume":"146","author":"Siden-Kiamos","year":"2022","journal-title":"Insect Biochem. Mol. Biol"},{"key":"2026100311293411000_ieag108-B71","doi-asserted-by":"publisher","first-page":"3199","DOI":"10.1002\/ps.5875","article-title":"Symbiosis disruption in the olive fruit fly, Bactrocera oleae (Rossi), as a potential tool for sustainable control","volume":"76","author":"Sinno","year":"2020","journal-title":"Pest Manag. Sci"},{"key":"2026100311293411000_ieag108-B72","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1002\/ps.1306","article-title":"Organophosphate resistance in olive fruit fly Bactrocera oleae populations in Greece and Cyprus","volume":"63","author":"Skouras","year":"2007","journal-title":"Pest Manag. Sci"},{"key":"2026100311293411000_ieag108-B73","doi-asserted-by":"publisher","first-page":"125","DOI":"10.3390\/biology14020125","article-title":"Utilizing olive fly ecology towards sustainable pest management","volume":"14","author":"Stavrianakis","year":"2025","journal-title":"Biology. (Basel)"},{"key":"2026100311293411000_ieag108-B74","doi-asserted-by":"publisher","first-page":"1681993","DOI":"10.3389\/fpls.2025.1681993","article-title":"Exploring morphological aspects, cuticle size and volatile compounds in the fruits of four olive cultivars as possibly interdependent components of Bactrocera oleae tolerance","volume":"16","author":"Taiti","year":"2025","journal-title":"Front. Plant Sci"},{"key":"2026100311293411000_ieag108-B75","doi-asserted-by":"publisher","first-page":"1408","DOI":"10.1111\/j.1462-2920.2006.01034.x","article-title":"Differential gene expression in response to phenol and catechol in Bacillus subtilis","volume":"8","author":"Tam","year":"2006","journal-title":"Environ. Microbiol"},{"key":"2026100311293411000_ieag108-B76","doi-asserted-by":"publisher","first-page":"11132","DOI":"10.1038\/s41598-022-15186-7","article-title":"Houseflies harbor less diverse microbiota under laboratory conditions but maintain a consistent set of host-associated bacteria","volume":"12","author":"Voulgari-Kokota","year":"2022","journal-title":"Sci. Rep"},{"key":"2026100311293411000_ieag108-B77","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: elegant graphics for data analysis","author":"Wickham","year":"2016"},{"key":"2026100311293411000_ieag108-B78","author":"Wickham","year":"2023"},{"key":"2026100311293411000_ieag108-B79","doi-asserted-by":"publisher","first-page":"100388","DOI":"10.1016\/j.xinn.2023.100388","article-title":"MicrobiotaProcess: a comprehensive R package for deep mining microbiome data","volume":"4","author":"Xu","year":"2023","journal-title":"Innovation (Camb)"},{"key":"2026100311293411000_ieag108-B80","doi-asserted-by":"publisher","first-page":"116461","DOI":"10.1016\/j.envpol.2021.116461","article-title":"An efficient phthalate ester-degrading Bacillus subtilis: degradation kinetics, metabolic pathway, and catalytic mechanism of the key enzyme","volume":"273","author":"Xu","year":"2021","journal-title":"Environ. Pollut"},{"key":"2026100311293411000_ieag108-B81","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1186\/1471-2105-13-134","article-title":"Primer-BLAST: a tool to design target-specific primers for PCR","volume":"13","author":"Ye","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"2026100311293411000_ieag108-B82","doi-asserted-by":"publisher","first-page":"1177951","DOI":"10.3389\/fmicb.2023.1177951","article-title":"A comprehensive genomic analysis provides insights on the high environmental adaptability of Acinetobacter strains","volume":"14","author":"Zhao","year":"2023","journal-title":"Front. Microbiol"},{"key":"2026100311293411000_ieag108-B83","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jisesa\/ieaf005","article-title":"Assessing cultivar-specific susceptibility and morphological adaptations of Bactrocera oleae (Diptera: Tephritidae) in olive orchards","volume":"25","author":"\u017divkovi\u0107","year":"2025","journal-title":"J. Insect Sci"}],"container-title":["Journal of Insect Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jinsectscience\/article-pdf\/26\/5\/ieag108\/71576804\/ieag108.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jinsectscience\/article-pdf\/26\/5\/ieag108\/71576804\/ieag108.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:29:44Z","timestamp":1791041384000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jinsectscience\/article\/doi\/10.1093\/jisesa\/ieag108\/8860287"}},"subtitle":[],"editor":[{"given":"Nicoletta","family":"Faraone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":83,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jisesa\/ieag108","relation":{},"ISSN":["1536-2442"],"issn-type":[{"value":"1536-2442","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,10]]},"published":{"date-parts":[[2026,9,1]]},"article-number":"ieag108"}}