{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T11:52:24Z","timestamp":1784029944540,"version":"3.55.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T00:00:00Z","timestamp":1689897600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["2018-2479"],"award-info":[{"award-number":["2018-2479"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["2016-0648"],"award-info":[{"award-number":["2016-0648"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003947","name":"Universidad Nacional de La Plata","doi-asserted-by":"publisher","award":["11\/X695"],"award-info":[{"award-number":["11\/X695"]}],"id":[{"id":"10.13039\/501100003947","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Food allergies have become a health concern worldwide. Around 6% to 10% of children are allergic to cow's milk proteins. We have previously characterized colorectal polyps in patients sensitized to food allergens. These polyps are classified as inflammatory and present a type 2 environment, with elevated interleukin (IL)-13 and IL-4, and are a site of immunoglobulin E synthesis. In this study, we characterized and isolated cow's milk protein\u2013specific T cell lines and T cell clones from the lamina propria of polyps from patients sensitized to these proteins. Isolated T cells responded to cow's milk proteins similarly to peripheral blood T cells, showing antigen-specific cell proliferation and Th2 cytokines release in vitro. T cell clones obtained were all CD4+ T cells and expressed the membrane TCR\u03b1\u03b2 receptor and secreted higher IL-4, IL-5, and IL-13 amounts than unstimulated cells, whereas interferon \u03b3 secretion remained unchanged. Remarkably, the gut homing chemokine receptor CCR9 was augmented in cow\u2019s milk\u2013specific peripheral and lamina propria T cells, and CCL25 was found to be expressed in the inflammatory polyp tissue and not in the adjacent mucosa. In conclusion, we isolated and characterized cow\u2019s milk\u2013specific lamina propria CD4+ Th2 cells from colonic inflammatory polyps. CCR9 expression on these cells, along with increase secretion of CCL25 in the polyp, favors recruitment and cow's milk\u2013specific allergic response within the inflammatory polyp tissue. Our findings may be critical to understand the underlying mechanism that promotes immunoglobulin E synthesis in the colon of cow's milk proteins allergic patients, contributing to the development of novel T cell\u2013targeted immunotherapies.<\/jats:p>","DOI":"10.1093\/jleuko\/qiad083","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T19:35:22Z","timestamp":1689968122000},"page":"434-442","source":"Crossref","is-referenced-by-count":1,"title":["Food-sensitized pediatric patients show colonic cow's milk protein\u2013specific Th2 cells"],"prefix":"10.1093","volume":"114","author":[{"given":"Karina E","family":"Canziani","sequence":"first","affiliation":[{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos, CONICET \u2013 Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, asociado Comisi\u00f3n de Investigaciones Cient\u00edficas de la Provincia de Buenos Aires , Boulevard 120 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