{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:35:56Z","timestamp":1791293756787,"version":"4.1.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100020431","name":"Lemann Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020431","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Harvard University Lemann Brazil Research Fund"},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CNPq, Brazil","award":["406019\/2021-5"],"award-info":[{"award-number":["406019\/2021-5"]}]},{"name":"CNPq, Brazil","award":["307270\/2022-0"],"award-info":[{"award-number":["307270\/2022-0"]}]},{"name":"CNPq, Brazil","award":["APQ-00754-23"],"award-info":[{"award-number":["APQ-00754-23"]}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FAPEMIG, Brazil","award":["APQ-02930-21"],"award-info":[{"award-number":["APQ-02930-21"]}]},{"name":"FAPEMIG, Brazil","award":["APQ-00754-23"],"award-info":[{"award-number":["APQ-00754-23"]}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R37AI020241"],"award-info":[{"award-number":["R37AI020241"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Eosinophil sombrero vesicles are large tubular carriers resident in the cytoplasm of human eosinophils, identifiable by transmission electron microscopy, and important for immune mediator transport. Increased formation of sombrero vesicles occurs in activated eosinophils in vitro and in vivo. In tissue sites of eosinophilic cytolytic inflammation, extracellular eosinophil sombrero vesicles are noted, but their frequency and significance in eosinophil-associated diseases remain unclear. Here, we performed comprehensive quantitative transmission electron microscopy analyses and electron tomography to investigate the numbers, density, integrity, and 3-dimensional structure of eosinophil sombrero vesicles in different biopsy tissues from 5 prototypic eosinophil-associated diseases (eosinophilic chronic rhinosinusitis\/nasal sinuses, ulcerative colitis\/intestines, hypereosinophilic syndrome\/skin, dermatitis\/skin, and schistosomiasis\/rectum). The morphology of extracellular eosinophil sombrero vesicles was also compared with that of cytoplasmic eosinophil sombrero vesicles, isolated by subcellular fractionation from peripheral blood eosinophils. We demonstrated that (i) eosinophil cytolysis, releasing intact sombrero vesicles and membrane-bound granules, is a consistent event in all eosinophil-associated diseases; (ii) eosinophil sombrero vesicles persist intact even after complete disintegration of all cell organelles, except granules (late cytolysis); (iii) the eosinophil sombrero vesicle population, composed of elongated, curved, and typical sombreros, and the eosinophil sombrero vesicle 3-dimensional architecture, diameter, and density remain unchanged in the extracellular matrix; (iv) free eosinophil sombrero vesicles closely associate with extracellular granules; and (v) free eosinophil sombrero vesicles also associate with externalized chromatin during eosinophil ETosis. Remarkably, eosinophil sombrero vesicles appeared on the surface of other cells, such as plasma cells. Thus, eosinophil cytolysis\/ETosis can secrete intact sombrero vesicles, alongside granules, in inflamed tissues of eosinophil-associated diseases, potentially serving as propagators of eosinophil immune responses after cell death.<\/jats:p>","DOI":"10.1093\/jleuko\/qiae079","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T21:53:02Z","timestamp":1711403582000},"page":"398-408","source":"Crossref","is-referenced-by-count":12,"title":["Extracellular sombrero vesicles are hallmarks of eosinophilic cytolytic degranulation in tissue sites of human diseases"],"prefix":"10.1093","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5163-5714","authenticated-orcid":false,"given":"Vitor H","family":"Neves","sequence":"first","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, UFJF , Rua Jos\u00e9 Louren\u00e7o Kelmer, Juiz de Fora, MG 36036-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0983-0572","authenticated-orcid":false,"given":"Cinthia","family":"Palazzi","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, UFJF , Rua Jos\u00e9 Louren\u00e7o Kelmer, Juiz de Fora, MG 36036-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2203-2719","authenticated-orcid":false,"given":"K\u00e1ssia K","family":"Malta","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, UFJF , Rua Jos\u00e9 Louren\u00e7o Kelmer, Juiz de Fora, MG 36036-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0754-5578","authenticated-orcid":false,"given":"Kennedy","family":"Bonjour","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, UFJF , Rua Jos\u00e9 Louren\u00e7o Kelmer, Juiz de Fora, MG 36036-900 , Brazil"},{"name":"Unity of Biochemistry Membrane and Transport, Department of Cellular Biology and Infection, Institut Pasteur , 75724 Paris Cedex 15, Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2628-3224","authenticated-orcid":false,"given":"Felipe","family":"Kneip","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, UFJF , Rua Jos\u00e9 Louren\u00e7o Kelmer, Juiz de Fora, MG 36036-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3493-6929","authenticated-orcid":false,"given":"Felipe F","family":"Dias","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Biological Sciences, State University of Minas Gerais, UEMG , Avenida S\u00e3o Paulo 3996, Campus Ibirit\u00e9, MG 32400-000 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6645-1715","authenticated-orcid":false,"given":"Josiane S","family":"Neves","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, UFRJ , Avenida Carlos Chagas Filho 373, Rio de Janeiro, RJ 21941-971 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9580-560X","authenticated-orcid":false,"given":"Peter F","family":"Weller","sequence":"additional","affiliation":[{"name":"Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School , 330 Brookline Avenue, CLS 943, Boston, MA 02215 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1736-0806","authenticated-orcid":false,"given":"Rossana C N","family":"Melo","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, UFJF , Rua Jos\u00e9 Louren\u00e7o Kelmer, Juiz de Fora, MG 36036-900 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,3,25]]},"reference":[{"issue":"3","key":"2024072520153969900_qiae079-B1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/all.12103","article-title":"Eosinophil-derived cytokines in health and disease: unraveling novel mechanisms of selective 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Atlas of Eosinophil Cell Biology and Pathology","author":"Melo","year":"2022"},{"issue":"1","key":"2024072520153969900_qiae079-B6","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/JLB.3MR1217-476R","article-title":"Contemporary understanding of the secretory granules in human eosinophils","volume":"104","author":"Melo","year":"2018","journal-title":"J Leukoc Biol"},{"issue":"2","key":"2024072520153969900_qiae079-B7","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1189\/jlb.0707503","article-title":"Mechanisms of eosinophil secretion: large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins","volume":"83","author":"Melo","year":"2008","journal-title":"J Leukoc Biol"},{"issue":"2","key":"2024072520153969900_qiae079-B8","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.yexcr.2016.08.016","article-title":"Vesicular trafficking of immune mediators in human eosinophils revealed by immunoelectron 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