{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T13:39:04Z","timestamp":1793108344728,"version":"4.5.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T00:00:00Z","timestamp":1713225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"A.J.T.W"},{"name":"National Institute of Health and Care Research"},{"name":"Cambridge Biomedical Research Centre Core Biochemistry Assay Laboratory and Phenotyping Hub"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Intercellular cell adhesion molecule 1 (ICAM-1) is a cell surface glycoprotein with a vital role in the immune response to pathogens. The expression pattern of ICAM-1 is wide ranging, encompassing endothelial cells, epithelial cells, and neutrophils. Recent work has characterized the role of ICAM-1 in murine neutrophils, but the function of human neutrophil ICAM-1 is incompletely understood. Herein, we investigated the expression and role of ICAMs in human neutrophils in vitro and in vivo. Our findings show clear expression of ICAM-1, -3, and -4 on peripheral blood\u2013derived neutrophils and demonstrate that the pathogen-associated molecular pattern lipoteichoic acid is an inducer of ICAM-1 expression in vitro. In vivo, neutrophils obtained from the pleural cavity of patients with a parapneumonic effusion display enhanced expression of ICAM-1 compared with peripheral blood\u2013 and oral cavity\u2013derived neutrophils. Moreover, migration of peripheral blood\u2013derived neutrophils across endothelial cells can upregulate neutrophil ICAM-1 expression. These findings indicate that pathogen-associated molecular patterns and\/or cytokines, alongside transmigration, enhance neutrophil ICAM-1 expression at sites of inflammation. Mechanistically, we observed that ICAM-1high neutrophils display elevated S. aureus phagocytic capacity. However, unlike murine neutrophils, ICAM-1 intracellular signaling in human neutrophils was not essential for phagocytosis of Staphylococcus aureus and reactive oxygen species generation. Taken together, these results have important implications for the regulation of neutrophil-mediated pathogen clearance.<\/jats:p>","DOI":"10.1093\/jleuko\/qiae090","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T11:44:21Z","timestamp":1713181461000},"page":"901-908","source":"Crossref","is-referenced-by-count":28,"title":["Regulation of ICAM-1 in human neutrophils"],"prefix":"10.1093","volume":"116","author":[{"given":"Muralie","family":"Vignarajah","sequence":"first","affiliation":[{"name":"Wolfson Foundation Lung Injury Unit, Victor Phillip Dahdaleh Heart and Lung Research Institute, University of Cambridge School of Clinical Medicine , Papworth Road, Cambridge, CB2 0BB ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J T","family":"Wood","sequence":"additional","affiliation":[{"name":"Wolfson Foundation Lung Injury Unit, Victor Phillip Dahdaleh Heart and Lung Research Institute, University of Cambridge School of Clinical Medicine , Papworth Road, Cambridge, CB2 0BB ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Nelmes","sequence":"additional","affiliation":[{"name":"Wolfson Foundation Lung Injury Unit, Victor Phillip Dahdaleh Heart and Lung Research Institute, University of Cambridge School of Clinical Medicine , Papworth Road, Cambridge, CB2 0BB ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9243-0558","authenticated-orcid":false,"given":"Julien","family":"Subburayalu","sequence":"additional","affiliation":[{"name":"Wolfson Foundation Lung Injury Unit, Victor Phillip Dahdaleh Heart and Lung Research Institute, University of Cambridge School of Clinical Medicine , Papworth Road, Cambridge, CB2 0BB ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurgen","family":"Herre","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Cambridge University Hospitals NHS Foundation Trust , Hills Road, Cambridge, CB2 0QQ ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sussan","family":"Nourshargh","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of London , Charterhouse Square, London, EC1M 6BQ ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Summers","sequence":"additional","affiliation":[{"name":"Wolfson Foundation Lung Injury Unit, Victor Phillip Dahdaleh Heart and Lung Research Institute, University of Cambridge School of Clinical Medicine , Papworth Road, Cambridge, CB2 0BB ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin R","family":"Chilvers","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, Imperial College London , 72 Du Cane Rd, London, W12 0NN ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neda","family":"Farahi","sequence":"additional","affiliation":[{"name":"Wolfson Foundation Lung Injury Unit, Victor Phillip Dahdaleh Heart and Lung Research Institute, University of Cambridge School of Clinical Medicine , Papworth Road, Cambridge, CB2 0BB ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,4,16]]},"reference":[{"issue":"3","key":"2024100117242597600_qiae090-B1","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1007\/BF02786454","article-title":"The intercellular adhesion molecule (ICAM) family of proteins. 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