{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:55:05Z","timestamp":1753876505790,"version":"3.41.2"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92169102"],"award-info":[{"award-number":["92169102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"R&D Program of Guangzhou Laboratory","award":["SRPG22-006"],"award-info":[{"award-number":["SRPG22-006"]}]},{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"publisher","award":["2022B1515120043"],"award-info":[{"award-number":["2022B1515120043"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Cross disciplinary Major Research Program","award":["92374203"],"award-info":[{"award-number":["92374203"]}]},{"name":"Open Project Fund of Guangdong Provincial People\u2019s Hospital","award":["YKY-KF202208"],"award-info":[{"award-number":["YKY-KF202208"]}]},{"name":"Key R&D Program Key Special Projects for International Science and Technology Innovation Cooperation between Governments","award":["2023YFE0118700"],"award-info":[{"award-number":["2023YFE0118700"]}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["21623406"],"award-info":[{"award-number":["21623406"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Project of Scientific and Technological Bureau of Guangzhou City","award":["202201020335"],"award-info":[{"award-number":["202201020335"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>T cells are crucial for the normal functioning of the immune system. The development and response of these cells to foreign antigens involve many complex stages and interactions between various types of cells. However, many details of these processes are still unclear. Our research revealed a key role for a protein called ULK1, a serine\/threonine protein kinase, in regulating T-cell development and function. During T-cell maturation, the absence of Ulk1 (as in Ulk1\u2212\/\u2212 mice) leads to an increase in a cell type called DN3 in the thymus. We also found a reduction in the number of T cells in peripheral immune organs, such as the spleen, in Ulk1\u2212\/\u2212 mice. In response to Listeria infection, Ulk1\u2212\/\u2212 mice have a weaker ability to clear this bacterium, and their T cells also have defects in producing cytokines. However, the absence of Ulk1 did not affect the activation or apoptosis of naive CD4+ T cells in vitro. In a bone marrow chimeric mouse model, T cells from Ulk1\u2212\/\u2212 mice did not differ developmentally from those from control mice. Furthermore, RNA-seq revealed that Ulk1 deficiency affects the metabolic function of splenocytes and T-cell function in mice, potentially through the canonical Wnt signaling cascade and the ERK1\/ERK2 signaling cascades. Overall, these results suggest that Ulk1 is essential for T-cell maturation in the thymus, the balance of peripheral T cells, and the functional response of T cells to antigens.<\/jats:p>","DOI":"10.1093\/jleuko\/qiae183","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T08:30:50Z","timestamp":1724661050000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Ulk<\/i>1 regulates T cell development and response to <i>Listeria monocytogenes<\/i> stimulation"],"prefix":"10.1093","volume":"117","author":[{"given":"Yudai","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University , Guangzhou 510632 ,","place":["China"]},{"name":"Zhuhai Institute of Jinan University, Jinan University , Zhuhai 519070 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zijian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University , Guangzhou 510632 ,","place":["China"]},{"name":"Zhuhai Institute of Jinan University, Jinan University , Zhuhai 519070 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jieping","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Systems Biomedical Sciences, School of Medicine, Jinan University , Guangzhou 510632 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University , Guangzhou 510632 ,","place":["China"]},{"name":"Zhuhai Institute of Jinan University, Jinan University , Zhuhai 519070 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University , Guangzhou 510632 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8336-1703","authenticated-orcid":false,"given":"Guolong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, The Second Affiliated Hospital, School of Medicine, South China University of Technology , Guangzhou 510180 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2401-6168","authenticated-orcid":false,"given":"Guobing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University , Guangzhou 510632 ,","place":["China"]},{"name":"Guangdong-Hong Kong-Macau Great Bay Area Geroscience Joint Laboratory, School of Medicine, Jinan University , Guangzhou 510632 ,","place":["China"]},{"name":"Key Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education , Guangzhou 510632 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,8,26]]},"reference":[{"issue":"2","key":"2025010619021168000_qiae183-B1","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.immuni.2018.01.007","article-title":"Human T cell development, localization, and function throughout life","volume":"48","author":"Kumar","year":"2018","journal-title":"Immunity"},{"issue":"1","key":"2025010619021168000_qiae183-B2","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1038\/s41577-019-0203-y","article-title":"Metabolic coordination of T-cell quiescence and activation","volume":"20","author":"Chapman","year":"2020","journal-title":"Nat Rev Immunol"},{"issue":"3","key":"2025010619021168000_qiae183-B3","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1007\/s00281-019-00744-5","article-title":"CD8(+) T-cell exhaustion","volume":"41","author":"Kurachi","year":"2019","journal-title":"Semin Immunopathol"},{"issue":"11","key":"2025010619021168000_qiae183-B4","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1038\/nri3307","article-title":"Transcriptional control of effector and memory CD8+ T-cell differentiation","volume":"12","author":"Kaech","year":"2012","journal-title":"Nat Rev Immunol"},{"issue":"1","key":"2025010619021168000_qiae183-B5","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1146\/annurev-biochem-061516-044820","article-title":"Mechanisms of autophagy initiation","volume":"86","author":"Hurley","year":"2017","journal-title":"Annu Rev Biochem."},{"issue":"6","key":"2025010619021168000_qiae183-B6","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1042\/ebc20170021","article-title":"The mammalian ULK1 complex and autophagy initiation","volume":"61","author":"Zachari","year":"2017","journal-title":"Essays Biochem."},{"issue":"1","key":"2025010619021168000_qiae183-B7","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.jmb.2019.07.027","article-title":"Recruitment and activation of the ULK1\/Atg1 kinase complex in selective autophagy","volume":"432","author":"Turco","year":"2020","journal-title":"J Mol Biol."},{"issue":"6","key":"2025010619021168000_qiae183-B8","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1089\/ars.2018.7649","article-title":"P62\/SQSTM1 and selective autophagy in cardiometabolic diseases","volume":"31","author":"Jeong","year":"2019","journal-title":"Antioxid Redox Signal"},{"issue":"6","key":"2025010619021168000_qiae183-B9","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1038\/s41580-018-0003-4","article-title":"Mechanism and medical implications of mammalian autophagy","volume":"19","author":"Dikic","year":"2018","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"8","key":"2025010619021168000_qiae183-B10","doi-asserted-by":"publisher","first-page":"2462","DOI":"10.1016\/j.jmb.2019.10.028","article-title":"Autophagosome-lysosome fusion","volume":"432","author":"L\u0151rincz","year":"2020","journal-title":"J Mol Biol."},{"issue":"1","key":"2025010619021168000_qiae183-B11","doi-asserted-by":"publisher","first-page":"155","DOI":"10.4049\/jimmunol.2100452","article-title":"Prosurvival IL-7-stimulated weak strength of mTORC1-S6K controls T-cell memory via transcriptional FOXO1-TCF1-Id3 and metabolic AMPK\u03b11-ULK1-ATG7 pathways","volume":"208","author":"Xu","year":"2022","journal-title":"J Immunol"},{"issue":"1","key":"2025010619021168000_qiae183-B12","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/s13578-016-0098-2","article-title":"IL-15 signaling promotes adoptive effector T-cell survival and memory formation in irradiation-induced lymphopenia","volume":"6","author":"Xu","year":"2016","journal-title":"Cell Biosci"},{"issue":"17","key":"2025010619021168000_qiae183-B13","doi-asserted-by":"publisher","first-page":"9534","DOI":"10.3390\/ijms23179534","article-title":"The critical role of AMPK\u03b11 in regulating autophagy and mitochondrial respiration in IL-15-stimulated mTORC1(weak) signal-induced T-cell memory: an interplay between Yin (AMPK\u03b11) and Yang (mTORC1) energy sensors in T-cell differentiation","volume":"23","author":"Ara","year":"2022","journal-title":"Int J Mol Sci."},{"issue":"1","key":"2025010619021168000_qiae183-B14","doi-asserted-by":"publisher","first-page":"37","DOI":"10.3390\/ijms23010037","article-title":"The energy sensor AMPK\u03b11 is critical in rapamycin-inhibition of mTORC1-S6K-induced T-cell memory","volume":"23","author":"Ara","year":"2021","journal-title":"Int J Mol Sci."},{"issue":"51","key":"2025010619021168000_qiae183-B15","doi-asserted-by":"publisher","first-page":"eadj8442","DOI":"10.1126\/sciadv.adj8442","article-title":"PRKAG2.2 is essential for FoxA1(+) regulatory T-cell differentiation and metabolic rewiring distinct from FoxP3(+) regulatory T cells","volume":"9","author":"Mandatori","year":"2023","journal-title":"Sci Adv."},{"issue":"19","key":"2025010619021168000_qiae183-B16","doi-asserted-by":"publisher","first-page":"2441","DOI":"10.15252\/embj.201489838","article-title":"KIM-1-\/TIM-1-mediated phagocytosis links ATG5-\/ULK1-dependent clearance of apoptotic cells to antigen presentation","volume":"34","author":"Brooks","year":"2015","journal-title":"EMBO J."},{"issue":"3","key":"2025010619021168000_qiae183-B17","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1038\/s41577-018-0095-2","article-title":"Autophagy in the renewal, differentiation and homeostasis of immune cells","volume":"19","author":"Clarke","year":"2019","journal-title":"Nat Rev Immunol"},{"issue":"1","key":"2025010619021168000_qiae183-B18","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1084\/jem.20061303","article-title":"A critical role for the autophagy gene Atg5 in T-cell survival and proliferation","volume":"204","author":"Pua","year":"2007","journal-title":"J Exp Med."},{"key":"2025010619021168000_qiae183-B19","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.3389\/fimmu.2018.01023","article-title":"Cell-intrinsic roles for autophagy in modulating CD4 T-cell functions","volume":"9","author":"Jacquin","year":"2018","journal-title":"Front Immunol."},{"issue":"1","key":"2025010619021168000_qiae183-B20","doi-asserted-by":"publisher","first-page":"5951","DOI":"10.1038\/s41598-018-23993-0","article-title":"CD4 T-cell autophagy is integral to memory maintenance","volume":"8","author":"Murera","year":"2018","journal-title":"Sci Rep."},{"key":"2025010619021168000_qiae183-B21","doi-asserted-by":"publisher","first-page":"e03706","DOI":"10.7554\/eLife.03706","article-title":"Autophagy is a critical regulator of memory CD8(+) T-cell formation","volume":"3","author":"Puleston","year":"2014","journal-title":"Elife"},{"issue":"12","key":"2025010619021168000_qiae183-B22","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.1038\/ni.3025","article-title":"Autophagy is essential for effector CD8(+) T-cell survival and memory formation","volume":"15","author":"Xu","year":"2014","journal-title":"Nat Immunol."}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jleukbio\/advance-article-pdf\/doi\/10.1093\/jleuko\/qiae183\/59023062\/qiae183.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/117\/1\/qiae183\/59023062\/qiae183.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/117\/1\/qiae183\/59023062\/qiae183.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T19:02:30Z","timestamp":1736190150000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/doi\/10.1093\/jleuko\/qiae183\/7741169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,26]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,12,31]]}},"URL":"https:\/\/doi.org\/10.1093\/jleuko\/qiae183","relation":{},"ISSN":["1938-3673"],"issn-type":[{"type":"electronic","value":"1938-3673"}],"subject":[],"published-other":{"date-parts":[[2025,1]]},"published":{"date-parts":[[2024,8,26]]},"article-number":"qiae183"}}