{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:24:40Z","timestamp":1793118280264,"version":"4.5.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["F31 DE031948"],"award-info":[{"award-number":["F31 DE031948"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01 DE28296"],"award-info":[{"award-number":["R01 DE28296"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Neutrophils are highly abundant in the gingival tissues where they play an essential role in immune homeostasis by preventing microbial invasion. Here, we show that the oral periodontal pathogen Porphyromonas gingivalis utilizes its cysteine proteases (gingipains) to disengage phagosomal antimicrobial capacity. Arginine gingipains are a subfamily of trypsin-like proteases produced by P. gingivalis that cleave several host proteins at arginine residues. We find that RgpB-mediated proteolysis of host proteins is not limited to the extracellular or plasma membrane-associated host proteins, but also results in the degradation of several intracellular proteins. Using 2D-difference gel electrophoresis coupled with mass spectrometry, we identified several cytoskeletal and cytoplasmic proteins, including metabolic enzymes and antimicrobial proteins such as neutrophil elastase, myeloperoxidase, and proteinase 3 within neutrophil granules that were cleaved by RgpB. Strikingly, despite the breakdown of multiple proteins, RgpB-treated neutrophils did not undergo apoptosis but increased integrin expression and underwent broad transcriptional changes consistent with proinflammatory programming. However, despite their primed status and augmented inflammatory capacity, RgpB-treated neutrophils were conducive to intracellular bacterial survival due to the reduced activity of granule proteins and oxidative burst. Thus, our data show a previously unknown role for P. gingivalis proteases in the attenuation of neutrophil microbicidal capacity via proteolysis of intracellular proteins.<\/jats:p>","DOI":"10.1093\/jleuko\/qiae209","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T08:43:04Z","timestamp":1727253784000},"source":"Crossref","is-referenced-by-count":11,"title":["Limited proteolysis of neutrophil granule proteins by the bacterial protease RgpB depletes neutrophil antimicrobial capacity"],"prefix":"10.1093","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5346-3728","authenticated-orcid":false,"given":"Kelley N","family":"Cooper","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Louisville , 505 S Hancock St, Louisville, KY 40202 ,","place":["United States"]},{"name":"Center for Microbial Pathogenesis, Nationwide Children's Hospital , 700 Children's Drive, WA4024, Columbus, OH 43205 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Wielento","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Gronostajowa 7, 30\u2013387 Krakow ,","place":["Poland"]},{"name":"Department of Microbiology, Tumor and Cell Biology, Centre for Translational Microbiome Research, Karolinska Institutet , Stockholm 17165 ,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savannah C","family":"Morris","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Nationwide Children's Hospital , 700 Children's Drive, WA4024, Columbus, OH 43205 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Terekhova","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, Washington University School of Medicine in St. Louis , 660 S Euclid Ave, St. Louis, MO 63110 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos J","family":"Rodriguez-Hernandez","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Nationwide Children's Hospital , 700 Children's Drive, WA4024, Columbus, OH 43205 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Potempa","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases, University of Louisville , 501 S Preston St, Louisville, KY 40202 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A","family":"Carey","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Nationwide Children's Hospital , 700 Children's Drive, WA4024, Columbus, OH 43205 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxim N","family":"Artyomov","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, Washington University School of Medicine in St. Louis , 660 S Euclid Ave, St. Louis, MO 63110 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Potempa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University , Gronostajowa 7, 30\u2013387 Krakow ,","place":["Poland"]},{"name":"Department of Oral Immunology and Infectious Diseases, University of Louisville , 501 S Preston St, Louisville, KY 40202 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6185-1915","authenticated-orcid":false,"given":"Juhi","family":"Bagaitkar","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, Nationwide Children's Hospital , 700 Children's Drive, WA4024, Columbus, OH 43205 ,","place":["United States"]},{"name":"Department of Pediatrics, The Ohio State University College of Medicine , 700 Children\u2018s Drive, Columbus, OH 43205 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,25]]},"reference":[{"issue":"1","key":"2025030420580847500_qiae209-B1","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1111\/imr.13152","article-title":"Neutrophils in the periodontium: interactions with pathogens and roles in tissue homeostasis and inflammation","volume":"314","author":"Uriarte","year":"2023","journal-title":"Immunol Rev"},{"issue":"2","key":"2025030420580847500_qiae209-B2","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.smim.2016.02.002","article-title":"Immune and regulatory functions of neutrophils in inflammatory bone 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