{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T21:44:27Z","timestamp":1771623867315,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GU 769\/10-1"],"award-info":[{"award-number":["GU 769\/10-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FU 356\/12-1"],"award-info":[{"award-number":["FU 356\/12-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC TRR332"],"award-info":[{"award-number":["CRC TRR332"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014690","name":"Ministerium f\u00fcr Kultur und Wissenschaft des Landes Nordrhein-Westfalen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014690","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Der Regierende B\u00fcrgermeister von Berlin, Senatskanzlei Wissenschaft und Forschung.\u02ee"},{"name":"Projekt DEAL"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Quantification of neutrophil counts is the most relevant assessment of cellular immunity in clinical practice. Patients with neutropenia are considered at risk and are categorized according to its severity. The incidence of febrile neutropenia varies, but patients with acute myeloid leukemia are traditionally considered at high risk, especially following myelotoxic treatments. To provide additional functional parameters, we investigated the ex vivo migration properties and morphology of neutrophils in 10 patients with acute myeloid leukemia using single-cell video-microscopy and discovered, in addition to neutropenia, highly pathological neutrophil migration patterns and polarization defects in patients with untreated acute myeloid leukemia. Neutrophil speed was the most sensitive parameter and significantly lower at leukemia diagnosis (9.067 vs 15.810\u2005\u00b5m\/min, P = 0.0025) compared to healthy controls (n = 46). Hematological remission was associated with improved neutrophil migration profiles, but these ultimately normalized only after hematopoietic cell transplantation. Five patients were followed up for long-term effects of hematopoietic cell transplantation for up to 24 mo. This is the first longitudinal ex vivo neutrophil migration study in patients with acute myeloid leukemia, followed by allogeneic hematopoietic cell transplantation. It identified functional neutrophil impairments beyond routine quantitative assessments, adding to the well-known quantitative impairment of neutropenia. HCT can reestablish functional neutrophils with healthy migration profiles in these patients.<\/jats:p>","DOI":"10.1093\/jleuko\/qiae250","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T04:19:10Z","timestamp":1736396350000},"source":"Crossref","is-referenced-by-count":1,"title":["Risk beyond neutropenia: insights into neutrophil migration from newly diagnosed AML until late after allogeneic stem cell transplantation"],"prefix":"10.1093","volume":"117","author":[{"given":"Z\u00fclal","family":"Cibir","sequence":"first","affiliation":[{"name":"Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen , Essen, 45141 Essen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Beer","sequence":"additional","affiliation":[{"name":"Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen , Essen, 45141 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Imaging, University Hospital Essen, University of Duisburg-Essen , Essen, 45141 Essen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haji","family":"Abdulla","sequence":"additional","affiliation":[{"name":"Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen , Essen, 45141 Essen ,","place":["Germany"]},{"name":"Department of Infectious Diseases, West German Center of Infectious Diseases, University Hospital Essen, Hufelandstr. 55, University of Duisburg-Essen , 45122 Essen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina Rosa","family":"Neuendorff","sequence":"additional","affiliation":[{"name":"Department of Hematology and Stem Cell Transplantation, West-German Cancer Center, University Hospital Essen, University of Duisburg-Essen , 45122 Essen ,","place":["Germany"]},{"name":"Department of Hematology and Oncology, Marienhospital University Hospital, Ruhr-University Bochum , 44721 Bochum ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Sonneck","sequence":"additional","affiliation":[{"name":"ISAS Leibniz-Institut f\u00fcr Analytische Wissenschaften , Otto-Hahn-Str. 6b, 44227 Dortmund ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lennart","family":"Kowitz","sequence":"additional","affiliation":[{"name":"ISAS Leibniz-Institut f\u00fcr Analytische Wissenschaften, Otto-Hahn-Str. 6b , 44227 Dortmund ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Riese","sequence":"additional","affiliation":[{"name":"Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen , Essen, 45141 Essen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali Ata","family":"Tuz","sequence":"additional","affiliation":[{"name":"Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen , Essen, 45141 Essen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxu","family":"Chen","sequence":"additional","affiliation":[{"name":"ISAS Leibniz-Institut f\u00fcr Analytische Wissenschaften , Otto-Hahn-Str. 6b, 44227 Dortmund ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxim","family":"Cherneha","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, West German Center of Infectious Diseases, University Hospital Essen, Hufelandstr. 55, University of Duisburg-Essen , 45122 Essen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dietrich W","family":"Beelen","sequence":"additional","affiliation":[{"name":"Department of Hematology and Stem Cell Transplantation, West-German Cancer Center, University Hospital Essen, University of Duisburg-Essen , 45122 Essen ,","place":["Germany"]},{"name":"Department of Infectious Diseases, West German Center of Infectious Diseases, University Hospital Essen, Hufelandstr. 55, University of Duisburg-Essen , 45122 Essen ,","place":["Germany"]},{"name":"Department of Hematology and Oncology, Marienhospital University , Ruhr-University Bochum, 44721 Bochum , ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H Christian","family":"Reinhardt","sequence":"additional","affiliation":[{"name":"Department of Hematology and Stem Cell Transplantation, West-German Cancer Center, University Hospital Essen, University of Duisburg-Essen , 45122 Essen ,","place":["Germany"]},{"name":"Department of Hematology and Oncology, Marienhospital University Hospital, Ruhr-University Bochum , 44721 Bochum ,","place":["Germany"]},{"name":"DKTK\u2014German Cancer Consortium, Site Essen-D\u00fcsseldorf , 45122 Essen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5534-6055","authenticated-orcid":false,"given":"Matthias","family":"Gunzer","sequence":"additional","affiliation":[{"name":"Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen , Essen, 45141 Essen ,","place":["Germany"]},{"name":"ISAS Leibniz-Institut f\u00fcr Analytische Wissenschaften , Otto-Hahn-Str. 6b, 44227 Dortmund ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1347-3360","authenticated-orcid":false,"given":"Amin T","family":"Turki","sequence":"additional","affiliation":[{"name":"Department of Hematology and Stem Cell Transplantation, West-German Cancer Center, University Hospital Essen, University of Duisburg-Essen , 45122 Essen ,","place":["Germany"]},{"name":"Computational Hematology Lab, Institute for Artificial 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