{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:40:01Z","timestamp":1790746801630,"version":"4.1.0"},"reference-count":91,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001170","name":"Cancer Council Western Australia","doi-asserted-by":"publisher","award":["APP1065493"],"award-info":[{"award-number":["APP1065493"]}],"id":[{"id":"10.13039\/501100001170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001170","name":"Cancer Council Western Australia","doi-asserted-by":"publisher","award":["CCWA ECCRoY 2016"],"award-info":[{"award-number":["CCWA ECCRoY 2016"]}],"id":[{"id":"10.13039\/501100001170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001801","name":"University of Western Australia","doi-asserted-by":"publisher","award":["RA\/1\/1200\/883"],"award-info":[{"award-number":["RA\/1\/1200\/883"]}],"id":[{"id":"10.13039\/501100001801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007427","name":"MPN Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007427","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ruby Red Foundation","award":["RA\/1\/3246\/2"],"award-info":[{"award-number":["RA\/1\/3246\/2"]}]},{"name":"Ruby Red Foundation","award":["RA\/1\/3472\/2"],"award-info":[{"award-number":["RA\/1\/3472\/2"]}]},{"name":"Ann Helene Toakley Charitable Endowment","award":["IPAP2019\/0291"],"award-info":[{"award-number":["IPAP2019\/0291"]}]},{"DOI":"10.13039\/501100001063","name":"Raine Medical Research Foundation","doi-asserted-by":"publisher","award":["RPG043-19"],"award-info":[{"award-number":["RPG043-19"]}],"id":[{"id":"10.13039\/501100001063","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Gunn Family National Career Development"},{"name":"Medical Research Council Clinical Research Training Fellowship","award":["MR\/P02002X\/1"],"award-info":[{"award-number":["MR\/P02002X\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Megakaryocyte emperipolesis is a biological process in which a cell penetrates and exists as a viable intact cell within another. It is a recognized morphological feature of myeloproliferative neoplasms (MPNs), in which neutrophils can be seen within megakaryocytes in bone marrow smears and sections. We aimed to determine whether neutrophil contents, specifically protein and RNA, are deposited within megakaryocytes due to emperipolesis. Evaluation of hematoxylin and eosin\u2013stained bone marrow showed that 84% of MPN patients (n = 163) had megakaryocyte emperipolesis, most notably in enlarged megakaryocytes and those with pyknotic\/condensed nuclei. Morphological assessment and immunohistochemical staining for CD15-neutrophil membrane antigen confirmed that majority of intramegakaryocytic cells were neutrophils, and that emperipolesis was more frequent in myelofibrosis patients and patients with pathologic reticulin. Furthermore, megakaryocytes in MPNs were observed to have intracellular positivity for neutrophil azurophilic granule protein myeloperoxidase (MPO) (n = 21 MPN patients) and specific granule lactoferrin (n = 56). Platelets were also used as a surrogate to establish if neutrophil contents had been transferred into megakaryocytes intracellularly of MPN patients, using mass spectrometry to assess protein and transcriptomic next-generation sequencing to assess messenger RNA (mRNA). A total of 109 neutrophil mRNA transcripts and 20 neutrophil granule proteins were upregulated in platelets of MPN patients compared with control subjects, including cathepsin-G and lactoferrin, with 5.1- and 4.6-fold increase in mRNA and 1.8- and 1.4-fold protein increases, respectively. This suggests that the transfer of neutrophil material occurs during emperipolesis in disease state, which could be a consequence of neutrophil degranulation or apoptosis.<\/jats:p>","DOI":"10.1093\/jleuko\/qiaf093","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T07:21:41Z","timestamp":1750663301000},"source":"Crossref","is-referenced-by-count":11,"title":["Megakaryocyte emperipolesis in myeloproliferative neoplasms: Are neutrophils friends or foes?"],"prefix":"10.1093","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4973-4502","authenticated-orcid":false,"given":"Ryan J","family":"Collinson","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9126-2166","authenticated-orcid":false,"given":"Matthew D","family":"Linden","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3462-7501","authenticated-orcid":false,"given":"Kathryn A","family":"Fuller","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynne","family":"Wilson","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bob","family":"Mirzai","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine WA , Nedlands, WA 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3128-5539","authenticated-orcid":false,"given":"Darren","family":"Boey","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5050-1223","authenticated-orcid":false,"given":"Zi Y","family":"Ng","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 ,","place":["Australia"]},{"name":"Department of Haematology, Royal Perth Hospital , Perth, WA 6000 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hun S","family":"Chuah","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine WA , Nedlands, WA 6009 ,","place":["Australia"]},{"name":"Department of Haematology, Royal Perth Hospital , Perth, WA 6000 ,","place":["Australia"]},{"name":"Department of Haematology, Rockingham General Hospital , Rockingham, WA 6168 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3741-5835","authenticated-orcid":false,"given":"Jacques A J","family":"Malherbe","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 ,","place":["Australia"]},{"name":"Department of Haematology, Fiona Stanley Hospital , Murdoch, WA 6150 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Howman","sequence":"additional","affiliation":[{"name":"Department of Haematology, Sir Charles Gairdner Hospital , Perth, WA 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael F","family":"Leahy","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine WA , Nedlands, WA 6009 ,","place":["Australia"]},{"name":"Department of Haematology, Royal Perth Hospital , Perth, WA 6000 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1089-3853","authenticated-orcid":false,"given":"M Hasib","family":"Sidiqi","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine WA , Nedlands, WA 6009 ,","place":["Australia"]},{"name":"Department of Haematology, Fiona Stanley Hospital , Murdoch, WA 6150 ,","place":["Australia"]},{"name":"Curtin Medical School, Curtin University , Bentley, WA 6102 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8716-3261","authenticated-orcid":false,"given":"Janine H","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Haematology, Heart and Lung Research Institute, University of Cambridge , Cambridge , Cambridge Biomedical Campus, CB2 0BB,","place":["United Kingdom"]},{"name":"National Health Service Blood and Transplant, Cambridge Biomedical Campus , Cambridge, CB2 0PT ,","place":["United Kingdom"]},{"name":"Department of Haematology, Barts Health NHS Trust , London, E1 1BB ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5250-0160","authenticated-orcid":false,"given":"Willem H","family":"Ouwehand","sequence":"additional","affiliation":[{"name":"Department of Haematology, Heart and Lung Research Institute, University of Cambridge , Cambridge, Cambridge Biomedical Campus, CB2 0BB ,","place":["United Kingdom"]},{"name":"National Health Service Blood and Transplant, Cambridge Biomedical Campus , Cambridge, CB2 0PT ,","place":["United Kingdom"]},{"name":"Department of Haematology, Cambridge University Hospitals, Cambridge Biomedical Campus , Cambridge, CB2 0RE ,","place":["United Kingdom"]},{"name":"Department of Haematology, University College London Hospitals , London, NW1 2BU ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1028-9376","authenticated-orcid":false,"given":"Wendy N","family":"Erber","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 ,","place":["Australia"]},{"name":"PathWest Laboratory Medicine WA , Nedlands, WA 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2146-8561","authenticated-orcid":false,"given":"Belinda B","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia , Crawley, WA 6009 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