{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T18:23:00Z","timestamp":1776190980849,"version":"3.50.1"},"reference-count":148,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Montefiore\u2013Einstein Comprehensive Cancer Center"},{"name":"Analytical Imaging Facility","award":["P30 CA013330"],"award-info":[{"award-number":["P30 CA013330"]}]},{"name":"Analytical Imaging Facility","award":["1S10OD016214-01A1"],"award-info":[{"award-number":["1S10OD016214-01A1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We present a novel classification system for murine thymic epithelial cells (TECs), identifying 11 distinct types, four in the thymic cortex and seven in the medulla, based on their spatial localization and unique ultrastructural features. As key stromal components of the thymic microenvironment, TECs play indispensable roles in T cell development, including thymocyte selection, antigen presentation, and structural support. Our classification spans from the subcapsular cortex to the deep medulla and incorporates microanatomical context, morphology, and functional characteristics, providing a comprehensive and flexible framework to study TEC heterogeneity in relation to thymopoiesis. Aligning with TEC classification in rats and humans, this system highlights conserved spatial organization across species while remaining adaptable for refinement. Each TEC type is distinguished by features such as chromatin organization, cytoplasmic morphology, vacuolar content, and organelle distribution, attributes that suggest distinct functional contributions to various stages of thymocyte maturation. Importantly, the classification is designed for logical expansion both horizontally (inclusion of additional subtypes within the proposed TEC types) and vertically (inclusion of entirely novel TEC types). By integrating detailed morphological observations with testable functional hypotheses, this framework underscores the essential role of TEC diversity in supporting thymic architecture and orchestrating effective T cell output. Overall, it offers a robust foundation for future research into immune development and the pathological consequences of thymic dysfunction.<\/jats:p>","DOI":"10.1093\/jleuko\/qiaf115","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T07:53:05Z","timestamp":1756453985000},"source":"Crossref","is-referenced-by-count":3,"title":["Mapping murine thymic epithelial cells: functional ultrastructure and implications for thymopoiesis"],"prefix":"10.1093","volume":"117","author":[{"given":"Stepan","family":"Vodopyanov","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Tumor Microenvironment and Metastasis Program, Montefiore-Einstein Comprehensive Cancer Center , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie","family":"Gunther-Cummins","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Department of Cell Biology, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Montefiore-Einstein Comprehensive Cancer Center , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Churaman","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Montefiore-Einstein Comprehensive Cancer Center , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xheni","family":"Nishku","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Montefiore-Einstein Comprehensive Cancer Center , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theofilos","family":"Poutahidis","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki , University Campus, Thessaloniki 54124 ,","place":["Greece"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandros","family":"Hardas","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Population Sciences, The Royal Veterinary College , Hawkshead Lane, North Mymms, Hatfield AL9 7TA ,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank P","family":"Macaluso","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Department of Cell Biology, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Montefiore-Einstein Comprehensive Cancer Center , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8808-707X","authenticated-orcid":false,"given":"George S","family":"Karagiannis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Tumor Microenvironment and Metastasis Program, Montefiore-Einstein Comprehensive Cancer Center , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Integrated Imaging Program for Cancer Research, Montefiore-Einstein Comprehensive Cancer Center , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Cancer Dormancy Institute, Montefiore-Einstein Comprehensive Cancer Center , 1300 Morris Park Ave, Bronx, NY 10461 ,","place":["United States"]},{"name":"Marilyn and Stanley M. 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