{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T16:22:43Z","timestamp":1764174163137,"version":"3.46.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012264","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 EY14362","R01 GM098973"],"award-info":[{"award-number":["R01 EY14362","R01 GM098973"]}],"id":[{"id":"10.13039\/501100012264","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Ophthalmology"},{"name":"Prevent Blindness Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Assembly of NADPH oxidase 2 (NOX2) proteins in neutrophils plays an essential role in controlling microbial infections by producing high levels of reactive oxygen species (ROS). We report that neutrophils and NOX2 are required to control Pseudomonas aeruginosa in a clinically relevant murine model of blinding corneal infection. Given the published role for the voltage-gated proton channel Hv1 in sustaining NOX2-mediated ROS production, we examined the role of Hv1 in P. aeruginosa keratitis. Hvcn1\u2212\/\u2212 mice exhibited an impaired ability to kill bacteria that was associated with reduced neutrophil recruitment to infected corneas. However, in contrast to earlier reports, we found that Hvcn1\u2212\/\u2212 neutrophils produce more rather than less ROS compared with control, wild type neutrophils infected with P. aeruginosa or stimulated with PMA or zymosan. Collectively, we demonstrate that Hv1 has an important role in control of bacterial growth by neutrophils in bacterial infection beyond the regulation of ROS production.<\/jats:p>","DOI":"10.1093\/jleuko\/qiaf147","type":"journal-article","created":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T11:42:47Z","timestamp":1760787767000},"source":"Crossref","is-referenced-by-count":0,"title":["An essential role for the Hv1 voltage-gated proton channel in\n                    <i>Pseudomonas aeruginosa<\/i>\n                    corneal infections"],"prefix":"10.1093","volume":"117","author":[{"given":"Priscila","family":"Rodriguez","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of California, Irvine , 843 Health Sciences Road, Irvine, CA 92697 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela E","family":"Marshall","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of California, Irvine , 843 Health Sciences Road, Irvine, CA 92697 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jolynn","family":"Tran Chau","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of California, Irvine , 843 Health Sciences Road, Irvine, CA 92697 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Abdelmeseh","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of California, Irvine , 843 Health Sciences Road, Irvine, CA 92697 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6240-3209","authenticated-orcid":false,"given":"Jamison L","family":"Nourse","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of California, Irvine , 843 Health Sciences Road, Irvine, CA 92697 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Tombola","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of California, Irvine , 843 Health Sciences Road, Irvine, CA 92697 ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0137-7582","authenticated-orcid":false,"given":"Eric","family":"Pearlman","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of California, Irvine , 843 Health Sciences Road, Irvine, CA 92697 ,","place":["United States"]},{"name":"Department of Ophthalmology, University of California, Irvine , 843 Health Sciences Road, Irvine, CA 92697 ,","place":["United 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