{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T22:01:41Z","timestamp":1785276101399,"version":"3.55.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001502","name":"Department of Atomic Energy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001502","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cold atmospheric plasma (CAP) has garnered substantial attention in biomedical science, owing to its wide therapeutic applications in wound healing, disinfection, dentistry, cancer care, and inflammation. The anti-inflammatory effects of CAP have been studied, but, to our knowledge, there are no reports investigating its effect on T cell hyperactivation\u2013associated pathologies. This study examines CAP's impact on antigen-driven and homeostatic T cell proliferation and its potential to prevent acute graft-vs-host disease (GvHD). CAP treatment significantly attenuated GvHD-associated mortality and morbidity in mice. CAP inhibited stimulation-induced T cell activation, surface marker expression, cytokine secretion, and proliferation, without inducing cell death, indicating noncytotoxic immunomodulation. CAP modulated cellular redox, and pretreatment with N-acetylcysteine\u2013 or PEGylated catalase\u2013abrogated CAP-mediated suppression of mitogen-induced T cell responses. Moreover, CAP-mediated inhibition of stimulation-induced T cell responses was associated with suppression of the immune-regulatory, redox-sensitive transcription factors NF-\u03baB and Nrf2. These findings underscore immunotherapeutic potential of CAP in treatment of disorders linked to T cell hyperactivation.<\/jats:p>","DOI":"10.1093\/jleuko\/qiag066","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T11:37:32Z","timestamp":1782992252000},"source":"Crossref","is-referenced-by-count":0,"title":["Cold atmospheric plasma as an immunomodulator: suppression of T-cell hyperactivation and graft-vs-host disease via redox regulation"],"prefix":"10.1093","volume":"118","author":[{"given":"Thoh","family":"Maikho","sequence":"first","affiliation":[{"name":"Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre , Trombay, Mumbai 400085 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghavendra S","family":"Patwardhan","sequence":"additional","affiliation":[{"name":"Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre , Trombay, Mumbai 400085 ,","place":["India"]},{"name":"Radiobiology & Omics Lab, Radiological Research Unit, Bhabha Atomic Research Centre Facility , Kharghar, Navi Mumbai 410210 ,","place":["India"]},{"name":"Homi Bhabha National Institute, Training School Complex , Anushaktinagar, Mumbai 400094 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binita Kislay","family":"Kumar","sequence":"additional","affiliation":[{"name":"Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre , Trombay, Mumbai 400085 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Sharma","sequence":"additional","affiliation":[{"name":"Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre , Trombay, Mumbai 400085 ,","place":["India"]},{"name":"Radiobiology & Omics Lab, Radiological Research Unit, Bhabha Atomic Research Centre Facility , Kharghar, Navi Mumbai 410210 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8852-4180","authenticated-orcid":false,"given":"Santosh K","family":"Sandur","sequence":"additional","affiliation":[{"name":"Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre , Trombay, Mumbai 400085 ,","place":["India"]},{"name":"Radiobiology & Omics Lab, Radiological Research Unit, Bhabha Atomic Research Centre Facility , Kharghar, Navi Mumbai 410210 ,","place":["India"]},{"name":"Homi Bhabha National Institute, Training School Complex , Anushaktinagar, Mumbai 400094 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"2026072817565790700_qiag066-B1","doi-asserted-by":"publisher","first-page":"e0223925","DOI":"10.1371\/journal.pone.0223925","article-title":"Antibacterial efficacy of cold atmospheric plasma against Enterococcus faecalis planktonic cultures and biofilms in vitro","volume":"14","author":"Theinkom","year":"2019","journal-title":"PLoS One"},{"key":"2026072817565790700_qiag066-B2","doi-asserted-by":"publisher","first-page":"e22568","DOI":"10.1016\/j.heliyon.2023.e22568","article-title":"Applications of cold atmospheric plasma in immune-mediated inflammatory diseases via redox homeostasis: evidence and prospects","volume":"9","author":"Ma","year":"2023","journal-title":"Heliyon"},{"key":"2026072817565790700_qiag066-B3","doi-asserted-by":"publisher","first-page":"4479","DOI":"10.1038\/s41598-017-04770-x","article-title":"The specific vulnerabilities of cancer cells to the cold atmospheric plasma-stimulated solutions","volume":"7","author":"Yan","year":"2017","journal-title":"Sci Rep"},{"key":"2026072817565790700_qiag066-B4","doi-asserted-by":"publisher","first-page":"e0133120","DOI":"10.1371\/journal.pone.0133120","article-title":"Cold atmospheric plasma induces a predominantly necrotic cell death via the microenvironment","volume":"10","author":"Virard","year":"2015","journal-title":"PLoS One"},{"key":"2026072817565790700_qiag066-B5","doi-asserted-by":"publisher","first-page":"e0169457","DOI":"10.1371\/journal.pone.0169457","article-title":"Cold atmospheric plasma, created at the tip of an elongated flexible capillary using low electric current, can slow the progression of melanoma","volume":"12","author":"Binenbaum","year":"2017","journal-title":"PLoS One"},{"key":"2026072817565790700_qiag066-B6","doi-asserted-by":"publisher","first-page":"5216","DOI":"10.3390\/ijms20205216","article-title":"Disinfection and sterilization using plasma technology: fundamentals and future perspectives for biological applications","volume":"20","author":"Sakudo","year":"2019","journal-title":"Int J Mol Sci"},{"key":"2026072817565790700_qiag066-B7","first-page":"275","volume-title":"Cold atmospheric plasma treatment of infected skin tissue: evaluation of sterility, viability, and integrity","author":"Laurita","year":"2017"},{"key":"2026072817565790700_qiag066-B8","doi-asserted-by":"publisher","first-page":"7071536","DOI":"10.1155\/2019\/7071536","article-title":"Improved wound healing of airway epithelial cells is mediated by cold atmospheric plasma: a time course-related proteome analysis","volume":"2019","author":"Scharf","year":"2019","journal-title":"Oxid Med Cell Longev"},{"key":"2026072817565790700_qiag066-B9","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.cpme.2013.06.001","article-title":"Cold atmospheric argon plasma treatment may accelerate wound healing in chronic wounds: results of an open retrospective randomized controlled study in vivo","volume":"1","author":"Isbary","year":"2013","journal-title":"Clin Plasma Med."},{"key":"2026072817565790700_qiag066-B10","doi-asserted-by":"publisher","first-page":"5560","DOI":"10.3390\/app9245560","article-title":"The role of thermal effects in plasma medical applications: biological and calorimetric analysis","volume":"9","author":"Cordaro","year":"2019","journal-title":"Applied Sciences"},{"key":"2026072817565790700_qiag066-B11","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1615\/PlasmaMed.2014011997","article-title":"Quantitative assessment of blood coagulation by cold atmospheric plasma","volume":"4","author":"Heslin","year":"2015","journal-title":"Plasma Med."},{"key":"2026072817565790700_qiag066-B12","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.cpme.2017.10.001","article-title":"Platelets are key in cold physical plasma-facilitated blood coagulation in mice","volume":"7-8","author":"Bekeschus","year":"2017","journal-title":"Clin Plasma Med."},{"key":"2026072817565790700_qiag066-B13","doi-asserted-by":"publisher","first-page":"15977","DOI":"10.18632\/oncotarget.13304","article-title":"Cold atmospheric plasma, a novel promising anti-cancer treatment modality","volume":"8","author":"Yan","year":"2017","journal-title":"Oncotarget"},{"key":"2026072817565790700_qiag066-B14","doi-asserted-by":"publisher","first-page":"057101","DOI":"10.1063\/1.4801516","article-title":"Cold atmospheric plasma in cancer therapy","volume":"20","author":"Keidar","year":"2013","journal-title":"Phys Plasmas."},{"key":"2026072817565790700_qiag066-B15","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.cpme.2013.08.001","article-title":"Plasma in cancer treatment","volume":"1","author":"Schlegela","year":"2013","journal-title":"Clin Plasma Med."},{"key":"2026072817565790700_qiag066-B16","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1038\/bjc.2011.386","article-title":"Cold plasma selectivity and the possibility of a paradigm shift in cancer therapy","volume":"105","author":"Keidar","year":"2011","journal-title":"Br J Cancer"},{"key":"2026072817565790700_qiag066-B17","doi-asserted-by":"publisher","first-page":"031010","DOI":"10.1116\/1.4962130","article-title":"Treatment of gastric cancer cells with nonthermal atmospheric plasma generated in water","volume":"11","author":"Chen","year":"2016","journal-title":"Biointerphases"},{"key":"2026072817565790700_qiag066-B18","doi-asserted-by":"publisher","first-page":"5121","DOI":"10.3390\/jcm12155121","article-title":"Cold plasma therapy in chronic wounds-A multicenter, randomized controlled clinical trial (Plasma on Chronic Wounds for Epidermal Regeneration Study): preliminary results","volume":"12","author":"Abu Rached","year":"2023","journal-title":"J Clin Med."},{"key":"2026072817565790700_qiag066-B19","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1007\/s10103-025-04662-x","article-title":"Effects of cold atmospheric plasma on cellular behavior in oral and dental tissues: a systematic review","volume":"40","author":"Kadkhodazadeh","year":"2025","journal-title":"Lasers Med Sci."},{"key":"2026072817565790700_qiag066-B20","doi-asserted-by":"publisher","first-page":"1528","DOI":"10.3390\/biom13101528","article-title":"The effectiveness of cold atmospheric plasma (CAP) on bacterial reduction in dental implants: a systematic review","volume":"13","author":"Alqutaibi","year":"2023","journal-title":"Biomolecules"},{"key":"2026072817565790700_qiag066-B21","doi-asserted-by":"publisher","first-page":"4131","DOI":"10.3390\/ijms23084131","article-title":"Applications of plasma-activated water in dentistry: a review","volume":"23","author":"Milhan","year":"2022","journal-title":"Int J Mol Sci"},{"key":"2026072817565790700_qiag066-B22","doi-asserted-by":"publisher","first-page":"20332","DOI":"10.1038\/srep20332","article-title":"Cold atmospheric plasma jet-generated RONS and their selective effects on normal and carcinoma cells","volume":"6","author":"Kim","year":"2016","journal-title":"Sci Rep"},{"key":"2026072817565790700_qiag066-B23","doi-asserted-by":"publisher","first-page":"1245049","DOI":"10.1155\/2016\/1245049","article-title":"Role of ROS and RNS sources in physiological and pathological conditions","volume":"2016","author":"Di Meo","year":"2016","journal-title":"Oxid Med Cell Longev"},{"key":"2026072817565790700_qiag066-B24","doi-asserted-by":"publisher","first-page":"3164734","DOI":"10.1155\/2016\/3164734","article-title":"The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults","volume":"2016","author":"Nita","year":"2016","journal-title":"Oxid Med Cell Longev"},{"key":"2026072817565790700_qiag066-B25","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/j.jare.2014.11.001","article-title":"Allogeneic hematopoietic stem cell transplantation for non-malignant hematological disorders","volume":"6","author":"Mahmoud","year":"2015","journal-title":"J Adv Res."},{"key":"2026072817565790700_qiag066-B26","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1586\/ehm.10.21","article-title":"Allogeneic hematopoietic cell transplantation: the state of the art","volume":"3","author":"Gyurkocza","year":"2010","journal-title":"Expert Rev Hematol."},{"key":"2026072817565790700_qiag066-B27","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1038\/s41572-023-00438-1","article-title":"Acute graft-versus-host disease","volume":"9","author":"Malard","year":"2023","journal-title":"Nat Rev Dis Primers."},{"key":"2026072817565790700_qiag066-B28","doi-asserted-by":"publisher","first-page":"2167","DOI":"10.1056\/NEJMra1609337","article-title":"Acute graft-versus-host disease\u2014biologic process, prevention, and therapy","volume":"377","author":"Zeiser","year":"2017","journal-title":"N Engl J Med."},{"key":"2026072817565790700_qiag066-B29","doi-asserted-by":"publisher","first-page":"100792","DOI":"10.1016\/j.blre.2020.100792","article-title":"Graft-versus-host disease prophylaxis: pathophysiology-based review on current approaches and future directions","volume":"48","author":"Martinez-Cibrian","year":"2021","journal-title":"Blood Rev."},{"key":"2026072817565790700_qiag066-B30","doi-asserted-by":"publisher","first-page":"2338","DOI":"10.1056\/NEJMoa2215943","article-title":"Post-transplantation cyclophosphamide-based graft-versus-host disease prophylaxis","volume":"388","author":"Bola\u00f1os-Meade","year":"2023","journal-title":"N Engl J Med."},{"key":"2026072817565790700_qiag066-B31","doi-asserted-by":"publisher","first-page":"101053","DOI":"10.1016\/j.blre.2023.101053","article-title":"Expanding post-transplant cyclophosphamide to matched unrelated donor transplants and beyond","volume":"62","author":"Gooptu","year":"2023","journal-title":"Blood Rev."},{"key":"2026072817565790700_qiag066-B32","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1182\/blood.2023020073","article-title":"Novel developments in the prophylaxis and treatment of acute GVHD","volume":"142","author":"Jamy","year":"2023","journal-title":"Blood"},{"key":"2026072817565790700_qiag066-B33","doi-asserted-by":"publisher","first-page":"1800","DOI":"10.1056\/NEJMoa1917635","article-title":"Ruxolitinib for glucocorticoid-refractory acute graft-versus-host disease","volume":"382","author":"Zeiser","year":"2020","journal-title":"N Engl J Med."},{"key":"2026072817565790700_qiag066-B34","doi-asserted-by":"publisher","first-page":"9636897241226573","DOI":"10.1177\/09636897241226573","article-title":"Withania somnifera extract mitigates experimental acute graft versus host disease without abrogating graft versus leukemia effect","volume":"33","author":"Gupta","year":"2024","journal-title":"Cell Transplant."},{"key":"2026072817565790700_qiag066-B35","doi-asserted-by":"publisher","first-page":"1592","DOI":"10.3390\/antiox11081592","article-title":"Cold atmospheric plasma targeting hematological malignancies: potentials and problems of clinical translation","volume":"11","author":"Gangemi","year":"2022","journal-title":"Antioxidants (Basel)"},{"key":"2026072817565790700_qiag066-B36","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1080\/10715762.2017.1423069","article-title":"Cold atmospheric plasma-modulated phorbol 12-myristate 13-acetate-induced differentiation of U937 cells to macrophage-like cells","volume":"52","author":"Maikho","year":"2018","journal-title":"Free Radic Res"},{"key":"2026072817565790700_qiag066-B37","doi-asserted-by":"publisher","first-page":"2536","DOI":"10.1109\/TPS.2018.2803823","article-title":"Design of cold plasma flexible brush and evaluation of its oxidizing plume","volume":"46","author":"Das","year":"2018","journal-title":"IEEE Trans Plasma Sci."},{"key":"2026072817565790700_qiag066-B38","doi-asserted-by":"publisher","first-page":"17023","DOI":"10.1074\/jbc.M601595200","article-title":"Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-\u03baB activation and NF-\u03baB-regulated gene products through modulation of p65 and I\u03baB\u03b1 kinase activation, leading to potentiation of apoptosis induced by cytokine and chemotherapeutic agents","volume":"281","author":"Sandur","year":"2006","journal-title":"J Biol Chem."},{"key":"2026072817565790700_qiag066-B39","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.freeradbiomed.2015.04.007","article-title":"Amelioration of radiation-induced hematopoietic syndrome by an antioxidant chlorophyllin through increased stem cell activity and modulation of hematopoiesis","volume":"85","author":"Suryavanshi","year":"2015","journal-title":"Free Radic Biol Med."},{"key":"2026072817565790700_qiag066-B40","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1007\/s10753-013-9768-y","article-title":"Plumbagin, a vitamin K3 analogue, abrogates lipopolysaccharide-induced oxidative stress, inflammation and endotoxic shock via NF-\u03baB suppression","volume":"37","author":"Checker","year":"2014","journal-title":"Inflammation"},{"key":"2026072817565790700_qiag066-B41","doi-asserted-by":"publisher","first-page":"57","DOI":"10.2144\/0000113854","article-title":"Effective flow cytometric phenotyping of cells using minimal amounts of antibody","volume":"53","author":"Sharma","year":"2012","journal-title":"BioTechniques"},{"key":"2026072817565790700_qiag066-B42","doi-asserted-by":"publisher","first-page":"1559494","DOI":"10.3389\/fimmu.2025.1559494","article-title":"The NF-\u03baB signaling network in the life of T cells","volume":"16","author":"Daniels","year":"2025","journal-title":"Front Immunol."},{"key":"2026072817565790700_qiag066-B43","doi-asserted-by":"publisher","first-page":"116177","DOI":"10.1016\/j.celrep.2025.116177","article-title":"Nrf2 drives activation-driven expansion of CD4+T cells by modulating glucose and glutamine metabolism","volume":"44","author":"Tripathi","year":"2025","journal-title":"Cell Rep."},{"key":"2026072817565790700_qiag066-B44","doi-asserted-by":"publisher","first-page":"102673","DOI":"10.1016\/j.dnarep.2019.102673","article-title":"Inflammation-induced DNA damage, mutations and cancer","volume":"83","author":"Kay","year":"2019","journal-title":"DNA Repair (Amst)."},{"key":"2026072817565790700_qiag066-B45","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4274\/tjh.galenos.2019.2019.0157","article-title":"Acute graft-versus-host disease: a brief review","volume":"37","author":"Alada\u011f","year":"2020","journal-title":"Turk J Haematol."},{"key":"2026072817565790700_qiag066-B46","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1182\/blood.2019000952","article-title":"Cytokines and costimulation in acute graft-versus-host disease","volume":"136","author":"Hill","year":"2020","journal-title":"Blood"},{"key":"2026072817565790700_qiag066-B47","doi-asserted-by":"publisher","first-page":"2319","DOI":"10.3389\/fimmu.2019.02319","article-title":"Interleukin-6 as biomarker for acute GvHD and survival after allogeneic transplant with post-transplant cyclophosphamide","volume":"10","author":"Greco","year":"2019","journal-title":"Front Immunol."},{"key":"2026072817565790700_qiag066-B48","doi-asserted-by":"publisher","first-page":"1862","DOI":"10.1016\/j.bbmt.2011.07.001","article-title":"Tocilizumab for the treatment of steroid refractory graft-versus-host disease","volume":"17","author":"Drobyski","year":"2011","journal-title":"Biol Blood Marrow Transplant."},{"key":"2026072817565790700_qiag066-B49","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1016\/j.bbmt.2009.07.015","article-title":"Dichotomous role of interferon-gamma in allogeneic bone marrow transplant","volume":"15","author":"Lu","year":"2009","journal-title":"Biol Blood Marrow Transplant."},{"key":"2026072817565790700_qiag066-B50","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.cotox.2017.11.002","article-title":"NF-\u03baB in oxidative stress","volume":"7","author":"Lingappan","year":"2018","journal-title":"Curr Opin Toxicol."}],"container-title":["Journal of Leukocyte Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jleukbio\/advance-article-pdf\/doi\/10.1093\/jleuko\/qiag066\/68689415\/qiag066.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/118\/7\/qiag066\/68689415\/qiag066.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jleukbio\/article-pdf\/118\/7\/qiag066\/68689415\/qiag066.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T21:57:06Z","timestamp":1785275826000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jleukbio\/article\/doi\/10.1093\/jleuko\/qiag066\/8724009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":50,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7,3]]}},"URL":"https:\/\/doi.org\/10.1093\/jleuko\/qiag066","relation":{},"ISSN":["1938-3673"],"issn-type":[{"value":"1938-3673","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,7]]},"published":{"date-parts":[[2026,7]]},"article-number":"qiag066"}}