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Here, we show that early-life oral vaccination with a heterologous rhesus rotavirus (RV) induces short-lived intestinal Trm that are negative by intravascular staining and express a CD44+CD69+CD103+\/\u2212P2X7R+ phenotype. Blocking lymphocyte homing with an anti-\u03b14\u03b27 antibody during vaccination did not alter the magnitude of RV-specific intestinal T cells or affect protection following murine RV challenge. In contrast, reduction of RV-specific intraepithelial lymphocytes by nicotinamide adenine dinucleotide treatment, or genetic deletion of the aryl hydrocarbon receptor repressor (Ahrr\u2212\/\u2212 mice)\u2014which also exhibit decreased intraepithelial CD8+ T cells after infection\u2014resulted in increased viral antigen shedding upon challenge. These results suggest that intestinal Trm cells induced by early-life vaccination may contribute to protection against RV infection.<\/jats:p>","DOI":"10.1093\/jleuko\/qiag080","type":"journal-article","created":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T07:32:44Z","timestamp":1781335964000},"source":"Crossref","is-referenced-by-count":0,"title":["Rotavirus-specific intestinal resident memory T cells induced by oral vaccination in early-life mice may contribute to protective immunity"],"prefix":"10.1093","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6878-7722","authenticated-orcid":false,"given":"Catherine","family":"Montenegro","sequence":"first","affiliation":[{"name":"Instituto de Gen\u00e9tica Humana, Facultad de Medicina, Pontificia Universidad Javeriana , Bogot\u00e1 110221 ,","place":["Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00edn Alonso","family":"Rond\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Clinical Epidemiology and Biostatistics, Facultad de Medicina, Pontificia Universidad Javeriana , Bogot\u00e1 110221 ,","place":["Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine , St.Louis, MO 63110 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Perdomo-Celis","sequence":"additional","affiliation":[{"name":"Instituto de Gen\u00e9tica Humana, Facultad de Medicina, Pontificia Universidad Javeriana , Bogot\u00e1 110221 ,","place":["Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0265-0563","authenticated-orcid":false,"given":"Manuel 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