{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T05:01:30Z","timestamp":1784523690613,"version":"3.55.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"7","funder":[{"DOI":"10.13039\/100020940","name":"National Institutes of Health grant","doi-asserted-by":"publisher","award":["R21 AI122103"],"award-info":[{"award-number":["R21 AI122103"]}],"id":[{"id":"10.13039\/100020940","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100021338","name":"Department of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100021338","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Eosinophil esophagitis (EoE) shares many epidemiologic and pathophysiologic characteristics with other allergic diseases, especially allergic asthma. Because rhinovirus (RV) infection is known to contribute to inflammation in allergic asthma, we sought to investigate whether RV might also contribute to esophageal inflammation in EoE through epithelial activation. In vitro cultures of primary esophageal epithelial cells and a human nontransformed immortalized esophageal epithelial cell line (EPC2-hTERT) grown submerged or at air-liquid interface were infected with RV-A1a or RV-A16. In addition, we added interleukin (IL)-13 to mimic EoE conditions. RV-binding receptors, RV replication, and interferon lambda-1 (IFNL1) response to infection were measured. RNA sequencing (RNA-seq) on RV-infected EPC2-hTERT cultures was performed for Gene Ontology analysis and compared with published RNA-seq data of esophageal biopsies from EoE patients. Both RV receptors were expressed by esophageal cells. After infection, we observed higher RV viral gene expression, indicative of active replication. IL-13 had little effect on replication in monolayers but was required in air-liquid interface for replication to occur. Interferon lambda-1 (IFNL1) messenger RNA was induced by RV-A1a in both cell types and IFNL1 protein was increased in IL-13\u2013treated EPC2-hTERT monolayers. Gene Ontology analysis of our in vitro experimental model highlighted virus-induced processes and increased IFN activity. In addition, we found overlapping genes between our RNA-seq results of EPC2-hTERT cells and publicly available RNA-seq data from esophageal biopsies of patients with EoE versus healthy control donors. Our data suggest the possibility that viruses such as RV may contribute to esophageal inflammation and play an important role in the inception and\/or persistence of EoE.<\/jats:p>","DOI":"10.1093\/jleuko\/qiag095","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T19:45:13Z","timestamp":1783712713000},"source":"Crossref","is-referenced-by-count":0,"title":["Rhinovirus infection may contribute to esophageal inflammation in eosinophilic esophagitis"],"prefix":"10.1093","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-5569-6995","authenticated-orcid":false,"given":"Paul S","family":"Fichtinger","sequence":"first","affiliation":[{"name":"Division of Allergy, Pulmonary and Critical Care, Department of Medicine, University of Wisconsin School of Medicine and Public Health , 600 Highland Avenue, Madison, WI 53792 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel G","family":"Sonsalla","sequence":"additional","affiliation":[{"name":"Division of Allergy, Pulmonary and Critical Care, Department of Medicine, University of Wisconsin School of Medicine and Public Health , 600 Highland Avenue, Madison, WI 53792 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5699-978X","authenticated-orcid":false,"given":"Mats W","family":"Johansson","sequence":"additional","affiliation":[{"name":"Division of Allergy, Pulmonary and Critical Care, Department of Medicine, University of Wisconsin School of Medicine and Public Health , 600 Highland Avenue, Madison, WI 53792 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3104-2823","authenticated-orcid":false,"given":"Amali E","family":"Samarasinghe","sequence":"additional","affiliation":[{"name":"Division of Allergy, Pulmonary and Critical Care, Department of Medicine, University of Wisconsin School of Medicine and Public Health , 600 Highland Avenue, Madison, WI 53792 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis D","family":"Lomeli","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, University of Wisconsin School of Medicine and Public Health , 600 Highland Avenue, Madison, WI 53792 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7656-0256","authenticated-orcid":false,"given":"Sameer K","family":"Mathur","sequence":"additional","affiliation":[{"name":"Division of Allergy, Pulmonary and Critical Care, Department of Medicine, University of Wisconsin School of Medicine and Public Health , 600 Highland Avenue, Madison, WI 53792 ,","place":["United 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