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Monocyte-derived DCs from 9 healthy female donors were exposed for 8\u2005h to 3 lysate pools generated from 27 high-grade serous ovarian tumors classified as immune-desert, immune-excluded, or immune-inflamed. Individual lysates showed phenotype-associated chemokine differences. The 3 pooled stimulation conditions elicited broadly conserved DC maturation responses and shared inflammatory transcriptional programs, with limited gene- and pathway-level differences. Permutational multivariate analysis attributed \u224858% of global transcriptional variance to donor identity (P = 0.001), whereas pooled lysate condition accounted for \u22484%, which was not significant (P = 0.756). Response magnitude varied continuously across donors and showed exploratory associations with baseline pathway activity in immature DCs. Under the tested conditions, early monocyte-derived DC activation was broadly shared across the 3 lysate pools, while the transcriptional response magnitude was more strongly associated with donor identity.<\/jats:p>","DOI":"10.1093\/jleuko\/qiag134","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:47:06Z","timestamp":1791978426000},"source":"Crossref","is-referenced-by-count":0,"title":["Monocyte-derived dendritic cell activation is broadly conserved across immune phenotype-specific ovarian tumor lysate pools, with donor-associated variation in response magnitude"],"prefix":"10.1093","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1325-4742","authenticated-orcid":false,"given":"Emilija","family":"Paberal\u0117","sequence":"first","affiliation":[{"name":"Laboratory of Immunology, National Cancer Institute , Petro Baublio g. 3B, Vilnius LT-08406 ,","place":["Lithuania"]},{"name":"Life Sciences Center, Vilnius University , 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