{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:34:49Z","timestamp":1790645689059,"version":"4.1.0"},"reference-count":10,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T00:00:00Z","timestamp":1600128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,10]]},"DOI":"10.1093\/jmcb\/mjaa047","type":"journal-article","created":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T07:21:06Z","timestamp":1599376866000},"page":"987-990","source":"Crossref","is-referenced-by-count":30,"title":["Rapid quantitative screening assay for SARS-CoV-2 neutralizing antibodies using HiBiT-tagged virus-like particles"],"prefix":"10.1093","volume":"12","author":[{"given":"Kei","family":"Miyakawa","sequence":"first","affiliation":[{"name":"Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sundararaj Stanleyraj","family":"Jeremiah","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihisa","family":"Ohtake","sequence":"additional","affiliation":[{"name":"Advanced Medical Research Center, Yokohama City University, Kanagawa 236-0004, Japan"},{"name":"Bioscience Division, Reagent Development Department, Tosoh Corporation, Kanagawa 252-1123, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoko","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaro","family":"Yamaoka","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan"},{"name":"Life Science Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Kanagawa 259-1146, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayuko","family":"Nishi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Saji","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Yokohama City University Hospital, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mototsugu","family":"Nishii","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Yokohama City University Hospital, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Kimura","sequence":"additional","affiliation":[{"name":"School of Medical Technology, Faculty of Health Sciences, Gunma Paz University, Gunma 370-0006, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Influenza Research Center, National Institute of Infectious Diseases, Tokyo 208-0011, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Yokohama City University Hospital, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihide","family":"Ryo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan"},{"name":"Advanced Medical Research Center, Yokohama City University, Kanagawa 236-0004, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,15]]},"reference":[{"key":"2021031110032200100_mjaa047-B1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.cell.2020.05.025","article-title":"Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients' B cells","volume":"182","author":"Cao","year":"2020","journal-title":"Cell"},{"key":"2021031110032200100_mjaa047-B2","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1126\/science.abc6952","article-title":"A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2","volume":"369","author":"Chi","year":"2020","journal-title":"Science"},{"key":"2021031110032200100_mjaa047-B3","doi-asserted-by":"crossref","first-page":"7001","DOI":"10.1073\/pnas.2002589117","article-title":"Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells","volume":"117","author":"Matsuyama","year":"2020","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2021031110032200100_mjaa047-B4","doi-asserted-by":"crossref","first-page":"4059","DOI":"10.1038\/s41467-020-17892-0","article-title":"A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation","volume":"11","author":"Muruato","year":"2020","journal-title":"Nat. Commun."},{"key":"2021031110032200100_mjaa047-B5","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1080\/22221751.2020.1743767","article-title":"Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2","volume":"9","author":"Nie","year":"2020","journal-title":"Emerg. Microbes. Infect."},{"key":"2021031110032200100_mjaa047-B6","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jaci.2020.05.020","article-title":"SARS-CoV-2 antibody testing-questions to be asked","volume":"146","author":"Ozcurumez","year":"2020","journal-title":"J. Allergy Clin. Immunol."},{"key":"2021031110032200100_mjaa047-B7","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.virusres.2017.10.015","article-title":"Development of a rapid and quantitative method for the analysis of viral entry and release using a NanoLuc luciferase complementation assay","volume":"243","author":"Sasaki","year":"2018","journal-title":"Virus Res."},{"key":"2021031110032200100_mjaa047-B8","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1038\/s41587-020-0631-z","article-title":"A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2-spike protein-protein interaction","volume":"38","author":"Tan","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"2021031110032200100_mjaa047-B9","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.cell.2020.02.058","article-title":"Structure, function, and antigenicity of the SARS-CoV-2 spike glycoprotein","volume":"181","author":"Walls","year":"2020","journal-title":"Cell"},{"key":"2021031110032200100_mjaa047-B10","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.bbrc.2004.07.084","article-title":"Oligomerization of the SARS-CoV S glycoprotein: dimerization of the N-terminus and trimerization of the ectodomain","volume":"322","author":"Xiao","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["Journal of Molecular Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jmcb\/advance-article-pdf\/doi\/10.1093\/jmcb\/mjaa047\/36111483\/mjaa047.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jmcb\/article-pdf\/12\/12\/987\/36546013\/mjaa047.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jmcb\/article-pdf\/12\/12\/987\/36546013\/mjaa047.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T06:40:26Z","timestamp":1615444826000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jmcb\/article\/12\/12\/987\/5906031"}},"subtitle":[],"editor":[{"given":"Haian","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,9,15]]},"references-count":10,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2020,9,15]]},"published-print":{"date-parts":[[2021,3,10]]}},"URL":"https:\/\/doi.org\/10.1093\/jmcb\/mjaa047","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.07.20.20158410","asserted-by":"object"}]},"ISSN":["1759-4685"],"issn-type":[{"value":"1759-4685","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,12,1]]},"published":{"date-parts":[[2020,9,15]]}}}