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In mammals, MARylation is mainly catalyzed by ADP-ribosyltransferases (ARTs), which consist of two groups: ART cholera toxin-like (ARTCs) and ART diphtheria toxin-like (ARTDs, also known as PARPs). The human ARTC (hARTC) family is composed of four members: two active mono-ADP-ARTs (hARTC1 and hARTC5) and two enzymatically inactive enzymes (hARTC3 and hARTC4). In this study, we systematically examined the homology, expression, and localization pattern of the hARTC family, with a particular focus on hARTC1. Our results showed that hARTC3 interacted with hARTC1 and promoted the enzymatic activity of hARTC1 by stabilizing hARTC1. We also identified vesicle-associated membrane protein-associated protein B (VAPB) as a new target of hARTC1 and pinpointed Arg50 of VAPB as the ADP-ribosylation site. Furthermore, we demonstrated that knockdown of hARTC1 impaired intracellular calcium homeostasis, highlighting the functional importance of hARTC1-mediated VAPB Arg50 ADP-ribosylation in regulating calcium homeostasis. In summary, our study identified a new target of hARTC1 in the endoplasmic reticulum and suggested that ARTC1 plays a role in regulating calcium signaling.<\/jats:p>","DOI":"10.1093\/jmcb\/mjad043","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:59:49Z","timestamp":1688000389000},"source":"Crossref","is-referenced-by-count":6,"title":["ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis"],"prefix":"10.1093","volume":"15","author":[{"given":"Xueyao","family":"Ma","sequence":"first","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyuan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1374-2201","authenticated-orcid":false,"given":"Xiaoyun","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Public Health, Affiliated Hospital of Hebei University , Baoding 071000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yipeng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayue","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9734-8772","authenticated-orcid":false,"given":"Xiuhua","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochun","family":"Yu","sequence":"additional","affiliation":[{"name":"Westlake Laboratory of Life Sciences and Biomedicine , Hangzhou 310024 , China"},{"name":"School of Life Sciences, Westlake University , Hangzhou 310024 , China"},{"name":"Institute of Biology, Westlake Institute for Advanced Study , Hangzhou 310024 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2791-7172","authenticated-orcid":false,"given":"Chen","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University , Baoding 071002 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,6,28]]},"reference":[{"key":"2024051011455079100_bib1","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/nrm1155","article-title":"Calcium signalling: dynamics, homeostasis and remodelling","volume":"4","author":"Berridge","year":"2003","journal-title":"Nat. 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