{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:38:42Z","timestamp":1787783922540,"version":"build-2784847793"},"reference-count":75,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T00:00:00Z","timestamp":1698451200000},"content-version":"vor","delay-in-days":27,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["BO4489\/1-1"],"award-info":[{"award-number":["BO4489\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["BO4489\/1-2"],"award-info":[{"award-number":["BO4489\/1-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["BO4489\/3-1"],"award-info":[{"award-number":["BO4489\/3-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Center for Molecular Medicine Cologne"},{"DOI":"10.13039\/501100000024","name":"Canadian Institute of Health Research","doi-asserted-by":"crossref","award":["CIHR-PJT\u00d152935"],"award-info":[{"award-number":["CIHR-PJT\u00d152935"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institute of Health Research","doi-asserted-by":"crossref","award":["178097"],"award-info":[{"award-number":["178097"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The novel coronavirus pandemic, first reported in December 2019, was caused by the severe acute respiratory syndrome coronavirus\u00a02 (SARS-CoV-2). SARS-CoV-2 infection leads to a strong immune response and activation of antigen-presenting cells, which can elicit acute respiratory distress syndrome (ARDS) characterized by the rapid onset of widespread inflammation, the so-called cytokine storm. In response to viral infections, monocytes are recruited into the lung and subsequently differentiate into dendritic cells (DCs). DCs are critical players in the development of acute lung inflammation that causes ARDS. Here, we focus on the interaction of a specific SARS-CoV-2 open reading frame protein, ORF8, with DCs. We show that ORF8 binds to DCs, causes pre-maturation of differentiating DCs, and induces the secretion of multiple proinflammatory cytokines by these cells. In addition, we identified DC-SIGN as a possible interaction partner of ORF8 on DCs. Blockade of ORF8 leads to reduced production of IL-1\u03b2, IL-6, IL-12p70, TNF-\u03b1, MCP-1 (also named CCL2), and IL-10 by DCs. Therefore, a neutralizing antibody blocking the ORF8-mediated cytokine and chemokine response could be an improved therapeutic strategy against SARS-CoV-2.<\/jats:p>","DOI":"10.1093\/jmcb\/mjad062","type":"journal-article","created":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T02:26:25Z","timestamp":1698459985000},"source":"Crossref","is-referenced-by-count":8,"title":["The unique ORF8 protein from SARS-CoV-2 binds to human dendritic cells and induces a hyper-inflammatory cytokine storm"],"prefix":"10.1093","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3744-4548","authenticated-orcid":false,"given":"Matthias","family":"Hamdorf","sequence":"first","affiliation":[{"name":"Cornea Lab Experimental Ophthalmology, Department of Ophthalmology, Faculty of Medicine and University Hospital Cologne , 50937 Cologne , Germany"},{"name":"Terasaki Institute for Biomedical Innovation , Los Angeles, CA 90024 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Imhof","sequence":"additional","affiliation":[{"name":"Center for Biochemistry, Faculty of Medicine and University Hospital Cologne , 50931 Cologne , Germany"},{"name":"Institute for Experimental Dentistry and Oral Musculoskeletal Biology, Faculty of Medicine and University Hospital Cologne , 50931 Cologne , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Bailey-Elkin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Manitoba , Winnipeg MB R3B 2E9 Manitoba , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janina","family":"Betz","sequence":"additional","affiliation":[{"name":"Center for Biochemistry, Faculty of Medicine and University Hospital Cologne , 50931 Cologne , Germany"},{"name":"Institute for Experimental Dentistry and Oral Musculoskeletal Biology, Faculty of Medicine and University Hospital Cologne , 50931 Cologne , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian J","family":"Theobald","sequence":"additional","affiliation":[{"name":"Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine and University Hospital Cologne , 50937 Cologne , Germany"},{"name":"Center for Molecular Medicine Cologne, University of Cologne , 50931 Cologne , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Simonis","sequence":"additional","affiliation":[{"name":"Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine and University Hospital Cologne , 50937 Cologne , Germany"},{"name":"Center for Molecular Medicine Cologne, University of Cologne , 50931 Cologne , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Di Cristanziano","sequence":"additional","affiliation":[{"name":"Institute of Virology, Faculty 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Site Bonn-Cologne , 50931 Cologne , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Augustin","sequence":"additional","affiliation":[{"name":"Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine and University Hospital Cologne , 50937 Cologne , Germany"},{"name":"Center for Molecular Medicine Cologne, University of Cologne , 50931 Cologne , Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Bonn-Cologne , 50931 Cologne , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Klein","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine Cologne, University of Cologne , 50931 Cologne , Germany"},{"name":"Institute of Virology, Faculty of Medicine and University Hospital Cologne , 50935 Cologne , Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Bonn-Cologne , 50931 Cologne , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A","family":"Alejandre Alcazar","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine Cologne, University of Cologne , 50931 Cologne , Germany"},{"name":"Department of Children and Adolescent Medicine, Faculty of Medicine and University Hospital Cologne , 50931 Cologne , Germany"},{"name":"Cologne Excellence Cluster Stress Responses in Aging-associated Diseases , 50931 Cologne , Germany"},{"name":"Institute for Lung Health (ILH), Universities of Gie\u00dfen and Marburg Lung Centre, Member of the German Center for Lung Research , 35392 Gie\u00dfen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Rongisch","sequence":"additional","affiliation":[{"name":"Dermatology, Faculty of Medicine and University Hospital Cologne , 50937 Cologne , 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