{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:29:02Z","timestamp":1787020142306,"version":"build-2736575974"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"am","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The nucleolus is a membraneless nuclear organelle formed by liquid\u2013liquid phase separation and serves as the hub for ribosomal RNA (rRNA) transcription and ribosome assembly. Here, we identify ALKBH3, a Fe(II)\/\u03b1-ketoglutarate-dependent dioxygenase, as a previously unrecognized nucleolar protein that colocalizes with the scaffold protein Nucleophosmin 1 (NPM1). Loss of ALKBH3 reduced nascent and precursor rRNA levels, impaired global protein translation, and suppressed cell proliferation. Although ALKBH3 lacked intrinsic LLPS capacity, it was recruited into NPM1 condensates via a direct interaction mediated by an N-terminal KRRRAR motif. Consistently, NPM1 knockdown diminished ALKBH3 nucleolar localization in cells, while in vitro assays demonstrated ALKBH3\u2013NPM1 co-condensation. In zebrafish, alkbh3 knockdown decreased pre-rRNA abundance and caused dose-dependent developmental delays. Together, these findings establish ALKBH3 as an NPM1-dependent nucleolar client protein critical for rRNA biogenesis, protein synthesis, and vertebrate development.<\/jats:p>","DOI":"10.1093\/jmcb\/mjag030","type":"journal-article","created":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T12:49:35Z","timestamp":1786798175000},"source":"Crossref","is-referenced-by-count":0,"title":["ALKBH3 is an NPM1-dependent nucleolar protein required for rRNA biogenesis and translation"],"prefix":"10.1093","author":[{"given":"Xixi","family":"Ma","sequence":"first","affiliation":[{"name":"Children\u2019s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases , Hangzhou 310052 ,","place":["China"]},{"name":"Department of Cell Biology, Zhejiang University School of Medicine , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuliang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Zhejiang University School of Medicine , Hangzhou 310058 ,","place":["China"]},{"name":"Innovation Center of Yangtze Delta, Zhejiang University , Jiaxing 314100 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Jin","sequence":"additional","affiliation":[{"name":"Children\u2019s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases , Hangzhou 310052 ,","place":["China"]},{"name":"Department of Cell Biology, Zhejiang University School of Medicine , Hangzhou 310058 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Children\u2019s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases , Hangzhou 310052 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangzhou","family":"Liu","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University , 310058 Hangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3968-3617","authenticated-orcid":false,"given":"Aifu","family":"Lin","sequence":"additional","affiliation":[{"name":"MOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University , 310058 Hangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhua","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Zhejiang University School of Medicine , Hangzhou 310058 ,","place":["China"]},{"name":"Department of Molecular Genetics, University of Toronto , Toronto, ON M5S 1A8 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Xie","sequence":"additional","affiliation":[{"name":"Children\u2019s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases , Hangzhou 310052 ,","place":["China"]},{"name":"Department of Cell Biology, Zhejiang University School of Medicine , Hangzhou 310058 ,","place":["China"]},{"name":"Zhejiang Key Laboratory of Neonatal Diseases , Hangzhou 310052 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,17]]},"container-title":["Journal of Molecular Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jmcb\/advance-article-pdf\/doi\/10.1093\/jmcb\/mjag030\/70672403\/mjag030.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jmcb\/advance-article-pdf\/doi\/10.1093\/jmcb\/mjag030\/70672403\/mjag030.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:34:01Z","timestamp":1787016841000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jmcb\/advance-article\/doi\/10.1093\/jmcb\/mjag030\/8762893"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/jmcb\/mjag030","relation":{},"ISSN":["1674-2788","1759-4685"],"issn-type":[{"value":"1674-2788","type":"print"},{"value":"1759-4685","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]},"article-number":"mjag030"}}