{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T04:38:12Z","timestamp":1781498292185,"version":"3.54.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T00:00:00Z","timestamp":1612396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"F\u00e9d\u00e9ration Nationale des Chasseurs","award":["FNC-PSN-PR5-2013"],"award-info":[{"award-number":["FNC-PSN-PR5-2013"]}]},{"name":"Ja\u00e9n University"},{"name":"Andalusian Plan for Scientific Research","award":["RNM-118"],"award-info":[{"award-number":["RNM-118"]}]},{"name":"Andalusian Plan for Scientific Research","award":["RNM-924"],"award-info":[{"award-number":["RNM-924"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Specific identification of oestrid larvae is usually problematic not only when using morphobiometric features, but also when applying molecular criteria, since very few molecular markers have been described for this group of flies. New molecular markers for oestrid are needed for more reliable species identification, diagnostic purposes, and epidemiological surveys; moreover, they can help in phylogenetic reconstruction. Here, we report the characterization of COI, 28S rDNA, ITS1, and ITS2 in Cephenemyia stimulator from roe deer and in Cephenemyia auribarbis and Pharyngomyia picta from red deer. The COI and 28S rDNA are very uniform in length, while the ITSs sequences are highly variable at both intraspecific and interspecific levels. The described ITSs sequences were longer than those described for other dipteran species by the presence of simple repeats and tandem repeat sequences. In C. auribarbis both ITS1 and ITS2 appeared as two variants, one short and the other long. In general, the analyzed markers present low intraspecific genetic variation and high interspecific variation. ITSs showed the greatest amount of intraspecific and interspecific variation. Phylogenetic analysis demonstrated that the characterized sequences differentiate the species and genera of Oestridae.<\/jats:p>","DOI":"10.1093\/jme\/tjab006","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T11:11:24Z","timestamp":1612782684000},"page":"1463-1469","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of New Molecular Markers of Three Botflies Parasitizing Cervid Hosts"],"prefix":"10.1093","volume":"58","author":[{"given":"Ana M","family":"de la Fuente","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Animal, Biolog\u00eda Vegetal y Ecolog\u00eda, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"},{"name":"Wildlife Ecology & Health Group (WE&H)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelia","family":"Caparr\u00f3s","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M","family":"Mora-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Molina","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebl","family":"Aleix-Mata","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Animal, Biolog\u00eda Vegetal y Ecolog\u00eda, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"},{"name":"Departamento de Biolog\u00eda Experimental, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roser","family":"Velarde","sequence":"additional","affiliation":[{"name":"Wildlife Ecology & Health Group (WE&H)"},{"name":"Servei d\u2019Ecopatologia de Fauna Salvatge (SEFaS), Departament de Medicina i Cirurgia Animals, Universitat Aut\u00f2noma de Barcelona, Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis E","family":"Fidalgo","sequence":"additional","affiliation":[{"name":"Departamento de Anatom\u00eda, Producci\u00f3n Animal y Ciencias Cl\u00ednicas Veterinarias, Universidad de Santiago de Compostela, Lugo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M","family":"L\u00f3pez-Beceiro","sequence":"additional","affiliation":[{"name":"Departamento de Anatom\u00eda, Producci\u00f3n Animal y Ciencias Cl\u00ednicas Veterinarias, Universidad de Santiago de Compostela, Lugo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9692-5870","authenticated-orcid":false,"given":"Pedro","family":"Lorite","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Boos","sequence":"additional","affiliation":[{"name":"Research Agency in Applied Ecology, Naturaconsta, Wilshausen, France"},{"name":"P\u00f4le Scientifique, F\u00e9d\u00e9ration Nationale des Chasseurs, 13 rue du G\u00e9n\u00e9ral Leclerc, Issy-Les-Moulineaux, Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Faure","sequence":"additional","affiliation":[{"name":"P\u00f4le Scientifique, F\u00e9d\u00e9ration Nationale des Chasseurs, 13 rue du G\u00e9n\u00e9ral Leclerc, Issy-Les-Moulineaux, Cedex, France"},{"name":"Commission Sanitaire, F\u00e9d\u00e9ration Nationale des Chasseurs, 13 rue du G\u00e9n\u00e9ral Leclerc, Issy-les-Moulineaux, Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas M","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Animal, Biolog\u00eda Vegetal y Ecolog\u00eda, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"},{"name":"Servei d\u2019Ecopatologia de Fauna Salvatge (SEFaS), Departament de Medicina i Cirurgia Animals, Universitat Aut\u00f2noma de Barcelona, Bellaterra, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6715-8158","authenticated-orcid":false,"given":"Antonio","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental, Universidad de Ja\u00e9n, Campus Las Lagunillas, s.n., Ja\u00e9n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"key":"2021051508393882200_CIT0001","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1002\/9780470377000.ch3","article-title":"Bot flies and warble flies (Order Diptera: Family Oestridae),","volume-title":"Parasitic diseases of wild mammals","author":"Colwell","year":"2001","edition":"2nd ed."},{"key":"2021051508393882200_CIT0002","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1079\/9780851996844.0220","article-title":"A synopsis of the biology, hosts, distribution, disease significance and management of the genera,","volume-title":"The oestrid flies: biology, host-parasite relationships, impact and management","author":"Colwell","year":"2006"},{"key":"2021051508393882200_CIT0003","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.forsciint.2014.07.018","article-title":"Molecular differentiation of Central European blowfly species (Diptera, Calliphoridae) using mitochondrial and nuclear genetic markers","volume":"242","author":"GilArriortua","year":"2014","journal-title":"Forensic Sci. Int"},{"key":"2021051508393882200_CIT0004","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser"},{"key":"2021051508393882200_CIT0005","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1046\/j.1471-8286.2003.00519.x","article-title":"Evolutionary conservation and versatility of a new set of primers for amplifying the ribosomal internal transcribed spacer regions in insects and other invertebrates","volume":"3","author":"Ji","year":"2003","journal-title":"Mol. Ecol. Notes"},{"key":"2021051508393882200_CIT0006","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. 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Macromol"},{"key":"2021051508393882200_CIT0011","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1007\/s10709-011-9621-x","article-title":"Evaluation of the internal transcribed spacer 2 (ITS2) as a molecular marker for phylogenetic inference using sequence and secondary structure information in blow flies (Diptera: Calliphoridae)","volume":"139","author":"Marinho","year":"2011","journal-title":"Genetica"},{"key":"2021051508393882200_CIT0012","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1016\/j.ympev.2012.08.007","article-title":"Molecular phylogenetics of Oestroidea (Diptera: Calyptratae) with emphasis on Calliphoridae: insights into the inter-familial relationships and additional evidence for paraphyly among blowflies","volume":"65","author":"Marinho","year":"2012","journal-title":"Mol. Phylogenet. 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