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Unfortunately, there is no efficient standard protocol for sterilizing mosquitoes that can produce a high level of sterility while maintaining mating ability and longevity. Therefore, we conducted a study of the critical factors necessary to develop such a standard protocol. In this study, male Ae. aegypti pupae, as well as adults aged 1 d and 3 d, were irradiated using a Gamma-cell 220 irradiator doses of 0, 20, 40, 60, 70, 80, and 100 Gray (Gy). In addition, male Ae. aegypti in the pupal and adult stage aged 1 d were irradiated at a dose of 70 Gy at various temperatures. Changes in emergence rates, longevity, sterility, and mating competitiveness were recorded for each combination of parameters. Results showed that an increase of irradiation dose leads to a rise of induced sterility at all developmental stages, while simultaneously reducing emergence rate, survival, and mating competitiveness. Higher temperatures resulted in increased levels of sterility, reduced longevity, and did not affect the ability to mate. This study found that an irradiation dose of 70 Gy at a temperature between 20.00 and 22.30\u00b0C administered in the pupal stage induced a high level of sterility (around 98%), while maintaining mating competitiveness and longevity.<\/jats:p>","DOI":"10.1093\/jme\/tjab166","type":"journal-article","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T15:34:50Z","timestamp":1633102490000},"page":"320-327","source":"Crossref","is-referenced-by-count":19,"title":["Investigation of Developmental Stage\/Age, Gamma Irradiation Dose, and Temperature in Sterilization of Male<i>Aedes aegypti<\/i>(Diptera: Culicidae) in a Sterile Insect Technique Program"],"prefix":"10.1093","volume":"59","author":[{"given":"Beni","family":"Ernawan","sequence":"first","affiliation":[{"name":"School of Life Sciences and Technology, Institut Teknologi Bandung (ITB), Jalan Ganesha No. 10, Bandung, Indonesia"},{"name":"Center for Isotopes and Radiation Application (CIRA), National Nuclear Energy Agency of Indonesia (BATAN), Jalan Lebak Bulus Raya No. 49, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tjandra","family":"Anggraeni","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Institut Teknologi Bandung (ITB), Jalan Ganesha No. 10, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sri","family":"Yusmalinar","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Institut Teknologi Bandung (ITB), Jalan Ganesha No. 10, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0619-497X","authenticated-orcid":false,"given":"Intan","family":"Ahmad","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Institut Teknologi Bandung (ITB), Jalan Ganesha No. 10, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,10,1]]},"reference":[{"key":"2022011306524886500_CIT0001","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/1-4020-4051-2_9","article-title":"Sterilizing insects with ionizing radiation,","volume-title":"Sterile insect technique principles and practice in area-wide integrated pest management","author":"Bakri","year":"2005"},{"key":"2022011306524886500_CIT0002","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1093\/jmedent\/47.4.581","article-title":"\u03b3 Ray dosimetry and mating capacity studies in the laboratory on Aedes albopictus males","volume":"47","author":"Balestrino","year":"2010","journal-title":"J. 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