{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:21:16Z","timestamp":1792790476389,"version":"4.5.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100009104","name":"Department of Health Research, India","doi-asserted-by":"publisher","award":["FUNDER_ID_501100009104V25011\/65\/2016-GIA-DHR"],"award-info":[{"award-number":["FUNDER_ID_501100009104V25011\/65\/2016-GIA-DHR"]}],"id":[{"id":"10.13039\/501100009104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Detection of Orientia tsutsugamushi DNA in a trombiculid mite chigger species suggests that it might be a potential vector of scrub typhus in an endemic area. Over a period of 20 mo, 85 rats were trapped, 57 had chiggers that were identified by standard morphometric techniques. The chigger pools were assessed by performing PCR assays targeting fragments of the single-copy genes 56 kDa type-specific antigen gene (TSA56) by nested PCR and the 47 kDa (htrA) quantitative real-time PCR (qPCR). The novel traD SYBR green assay that detects a multicopy gene was also performed. In total, 27 chigger pools were positive by traD qPCR, of which only 7 were positive by 47 kDa qPCR and in 3 of these, 56 kDa gene was amplified by nested PCR. Orientia tsutsugamushi-specific DNA was detected in Ascoschoengastia spp., Schoengastiella ligula, Leptotrombidium rajasthanense, Leptotrombidium deliense, and Leptotrombidium jayawickremei chigger pools. Therefore, they could be potential vectors of scrub typhus in Southern India. The three 56 kDa sequences belonged to TA716 genotype and Kato genogroup. Further studies are needed to confirm these chigger species as scrub typhus vectors in Northern Tamil Nadu.<\/jats:p>","DOI":"10.1093\/jme\/tjab180","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T14:08:59Z","timestamp":1638367739000},"page":"693-699","source":"Crossref","is-referenced-by-count":21,"title":["Detection of <i>Orientia tsutsugamushi<\/i> in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy <i>traD<\/i> Gene"],"prefix":"10.1093","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4561-8456","authenticated-orcid":false,"given":"John Antony Jude","family":"Prakash","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kannan","family":"Kamarasu","sequence":"additional","affiliation":[{"name":"Institute of Vector Control and Zoonoses, 150 Titan Jewellery Road, SIPCOT Phase I, Hosur Tamil Nadu 635126, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P Philip","family":"Samuel","sequence":"additional","affiliation":[{"name":"ICMR-Vector Control Research Centre Field Station, 4, Sarojini St, Chinna Chokikulam, Madurai Tamil Nadu 625002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Govindarajan","sequence":"additional","affiliation":[{"name":"ICMR-Vector Control Research Centre Field Station, 4, Sarojini St, Chinna Chokikulam, Madurai Tamil Nadu 625002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Punitha","family":"Govindasamy","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liji Anna","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Ramalingam","sequence":"additional","affiliation":[{"name":"Institute of Vector Control and Zoonoses, 150 Titan Jewellery Road, SIPCOT Phase I, Hosur Tamil Nadu 635126, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Nirmalson","sequence":"additional","affiliation":[{"name":"Institute of Vector Control and Zoonoses, 150 Titan Jewellery Road, SIPCOT Phase I, Hosur Tamil Nadu 635126, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K C","family":"Seran","sequence":"additional","affiliation":[{"name":"Department of Community Medicine, Madras Medical College, Near Park Town Station, Chennai, Tamil Nadu 600003, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,11,29]]},"reference":[{"key":"2022031611050366900_CIT0001","volume-title":"Taxonomic studies on Indian Muridae and Hystricidae: Mammalia, Rodentia. 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