{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:17:21Z","timestamp":1789730241700,"version":"4.0.1"},"reference-count":77,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004693","name":"Universiti Malaysia Kelantan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004595","name":"Universiti Sains Malaysia","doi-asserted-by":"publisher","award":["304\/PBIOLOGI\/650575"],"award-info":[{"award-number":["304\/PBIOLOGI\/650575"]}],"id":[{"id":"10.13039\/501100004595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004595","name":"Universiti Sains Malaysia","doi-asserted-by":"publisher","award":["USM-RU-PRGS 1001\/PBIOLOGI\/836003"],"award-info":[{"award-number":["USM-RU-PRGS 1001\/PBIOLOGI\/836003"]}],"id":[{"id":"10.13039\/501100004595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,14]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mosquito Aedes aegypti is the primary vector of the dengue, yellow fever, and chikungunya viruses. Evidence shows that Ae. aegypti males are polyandrous whereas Ae. aegypti females are monandrous in mating. However, the degree to which Ae. aegypti males and females can mate with different partners has not been rigorously tested. Therefore, this study examined the rates of polyandry via parentage assignment in three sets of competitive mating experiments using wild-type male and female Ae. aegypti. Parentage assignment was monitored using nine microsatellite DNA markers. All Ae. aegypti offspring were successfully assigned to parents with 80% or 95% confidence using CERVUS software. The results showed that both male and female Ae. aegypti mated with up to 3\u20134 different partners. Adults contributed differentially to the emergent offspring, with reproductive outputs ranging from 1 to 25 viable progeny. This study demonstrates a new perspective on the capabilities of male and female Ae. aegypti in mating. These findings are significant because successful deployment of reproductive control methods using genetic modification or sterile Ae. aegypti must consider the following criteria regarding their mating fitness: 1) choosing Ae. aegypti males that can mate with many different females; 2) testing how transformed Ae. aegypti male perform with polyandrous females; and 3) prioritizing the selection of polyandrous males and\/or females Ae. aegypti that have the most offspring.<\/jats:p>","DOI":"10.1093\/jme\/tjac081","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T03:30:41Z","timestamp":1655955041000},"page":"1525-1533","source":"Crossref","is-referenced-by-count":5,"title":["Parentage Assignment Using Microsatellites Reveals Multiple Mating in<i>Aedes aegypti<\/i>(Diptera: Culicidae): Implications for Mating Dynamics"],"prefix":"10.1093","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9065-5323","authenticated-orcid":false,"given":"Marcela","family":"Pimid","sequence":"first","affiliation":[{"name":"Faculty of Agro Based Industry, Universiti Malaysia Kelantan , Jeli Campus, 17600 Kelantan , Malaysia"},{"name":"School of Biological Sciences, Universiti Sains Malaysia , 11800 Penang , Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6729-1488","authenticated-orcid":false,"given":"Kumara Thevan","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Faculty of Agro Based Industry, Universiti Malaysia Kelantan , Jeli Campus, 17600 Kelantan , Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abu Hassan","family":"Ahmad","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Universiti Sains Malaysia , 11800 Penang , Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darlina","family":"Mohd Naim","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Universiti Sains Malaysia , 11800 Penang , Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey K","family":"Chambers","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Victoria University of Wellington , PO Box 600, 6140 Wellington , New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti Azizah","family":"Mohd Nor","sequence":"additional","affiliation":[{"name":"Institute of Marine Biotechnology, Universiti Malaysia Terengganu , 21030 Terengganu , Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5297-8981","authenticated-orcid":false,"given":"Abdul Hafiz","family":"Ab Majid","sequence":"additional","affiliation":[{"name":"Household & Structural Urban Entomology Laboratory, Vector Control Research Unit, School of Biological Sciences, Universiti Sains Malaysia , 11800 Penang , Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"key":"2022091418400828500_CIT0001","doi-asserted-by":"crossref","first-page":"691221","DOI":"10.3389\/fphys.2021.691221","article-title":"Male age influences re-mating incidence and sperm use in females of the dengue vector Aedes aegypti","volume":"12","author":"Agudelo","year":"2021","journal-title":"Front. Physiol"},{"key":"2022091418400828500_CIT0002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-38599-3","article-title":"Female resistance and harmonic convergence influence male mating success in Aedes aegypti","volume":"9","author":"Aldersley","year":"2019","journal-title":"Sci. Rep"},{"key":"2022091418400828500_CIT0003","doi-asserted-by":"crossref","first-page":"89","DOI":"10.4269\/ajtmh.1994.51.89","article-title":"Use of randomly amplified polymorphic DNA amplified by polymerase chain reaction markers to estimate the number of Aedes aegypti families at oviposition sites in San Juan, Puerto Rico","volume":"51","author":"Apostol","year":"1994","journal-title":"Am. J. Trop. Med"},{"key":"2022091418400828500_CIT0004","doi-asserted-by":"crossref","first-page":"2158244020982605","DOI":"10.1177\/2158244020982605","article-title":"Participatory: stakeholder\u2019s engagement toward dengue control techniques in Klang Valley, Malaysia","volume":"11","author":"Arham","year":"2021","journal-title":"SAGE Open"},{"key":"2022091418400828500_CIT0005","doi-asserted-by":"crossref","first-page":"259","DOI":"10.4238\/vol9-1gmr714","article-title":"Interpretation of electrophoretograms of seven microsatellite loci to determine the genetic diversity of the Arabian Oryx","volume":"9","author":"Arif","year":"2010","journal-title":"Genet. Mol. Res"},{"key":"2022091418400828500_CIT0006","doi-asserted-by":"crossref","first-page":"e26086","DOI":"10.1371\/journal.pone.0026086","article-title":"Cost of mating and insemination capacity of a genetically modified mosquito Aedes aegypti OX513A compared to its wild type counterpart","volume":"6","author":"Bargielowski","year":"2011","journal-title":"PLoS One"},{"key":"2022091418400828500_CIT0007","doi-asserted-by":"crossref","first-page":"2888","DOI":"10.1073\/pnas.1219599110","article-title":"Evolution of resistance to satyrization through reproductive character displacement in populations of invasive dengue vectors","volume":"110","author":"Bargielowski","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"2022091418400828500_CIT0008","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.meegid.2015.08.016","article-title":"Widespread evidence for interspecific mating between Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in nature","volume":"36","author":"Bargielowski","year":"2015","journal-title":"Infect. Genet. Evol"},{"key":"2022091418400828500_CIT0009","doi-asserted-by":"crossref","first-page":"3087","DOI":"10.1111\/j.1365-294X.2006.03014.x","article-title":"Molecular marker systems in insects: current trends and future avenues","volume":"15","author":"Behura","year":"2006","journal-title":"Mol. Ecol"},{"key":"2022091418400828500_CIT0010","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1093\/jmedent\/26.4.260","article-title":"Laboratory study of competition between United States strains of Aedes albopictus and Aedes aegypti (Diptera: Culicidae)","volume":"26","author":"Black","year":"1989","journal-title":"J. Med. Entomol"},{"key":"2022091418400828500_CIT0011","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0169-5347(03)00225-8","article-title":"DNA-based methods for pedigree reconstruction and kinship analysis in natural populations","volume":"18","author":"Blouin","year":"2003","journal-title":"Trends Ecol. Evol"},{"key":"2022091418400828500_CIT0012","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/j.1365-294X.1996.tb00329.x","article-title":"Use of microsatellite loci to classify individuals by relatedness","volume":"5","author":"Blouin","year":"1996","journal-title":"Mol. Ecol"},{"key":"2022091418400828500_CIT0013","doi-asserted-by":"crossref","first-page":"e42040","DOI":"10.1371\/journal.pone.0042040","article-title":"Evidence of multiple inseminations in the field in Aedes albopictus","volume":"7","author":"Boyer","year":"2012","journal-title":"PLoS One"},{"key":"2022091418400828500_CIT0014","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-71904-z","article-title":"Mating and blood-feeding induce transcriptome changes in the spermathecae of the yellow fever mosquito Aedes aegypti","volume":"10","author":"Camargo","year":"2020","journal-title":"Sci. Rep"},{"key":"2022091418400828500_CIT0015","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1093\/jme\/tjy158","article-title":"Spermathecal filling in Aedes aegypti and Aedes albopictus: effects of female and male body sizes and species","volume":"56","author":"Carrasquilla","year":"2019","journal-title":"J. Med. Entomol"},{"key":"2022091418400828500_CIT0016","doi-asserted-by":"crossref","first-page":"e0003864","DOI":"10.1371\/journal.pntd.0003864","article-title":"Suppression of a field population of Aedes aegypti in Brazil by sustained release of transgenic male mosquitoes","volume":"9","author":"Carvalho","year":"2015","journal-title":"PLoS Negl. Trop. Dis"},{"key":"2022091418400828500_CIT0017","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1126\/science.1081453","article-title":"Impact of genetic manipulation on the fitness of Anopheles stephensi mosquitoes","volume":"299","author":"Catteruccia","year":"2003","journal-title":"Science"},{"key":"2022091418400828500_CIT0018","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1093\/jhered\/esm015","article-title":"Microsatellite isolation and linkage group identification in the yellow fever mosquito Aedes aegypti","volume":"98","author":"Chambers","year":"2007","journal-title":"J. Hered"},{"key":"2022091418400828500_CIT0019","first-page":"745","article-title":"Polygamy: The possible significant behavior of Aedes aegypti and Aedes albopictus in relation to the efficient transmission of dengue virus","volume":"32","author":"Choochote","year":"2001","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"2022091418400828500_CIT0020","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1079\/9780851993133.0031","volume-title":"Biology of mosquitoes volume 2: sensory, reception and behavior.","author":"Clements","year":"1999"},{"key":"2022091418400828500_CIT0021","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1046\/j.1365-2915.2003.00424.x","article-title":"Natural skip oviposition of the mosquito Aedes aegypti indicated by codominant genetic markers","volume":"17","author":"Colton","year":"2003","journal-title":"Med. Vet. Entomol"},{"key":"2022091418400828500_CIT0022","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1126\/science.156.3781.1499","article-title":"Mosquitoes: Female monogamy induced by male accessory gland substance","volume":"156","author":"Craig","year":"1967","journal-title":"Science"},{"key":"2022091418400828500_CIT0023","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/sj.hdy.6800545","article-title":"Microsatellite null alleles in parentage analysis. J","volume":"93","author":"Dakin","year":"2004","journal-title":"Hered"},{"key":"2022091418400828500_CIT0024","first-page":"247","article-title":"Keys for the identification of the mosquitoes of Greece","volume":"13","author":"Darsie","year":"1997","journal-title":"J. Am. Mosq. Control Assoc"},{"key":"2022091418400828500_CIT0025","first-page":"780","article-title":"Polyandry depends on postmating time interval in the dengue vector Aedes aegypti. Am","volume":"94","author":"Degner","year":"2016","journal-title":"J. Trop. Med"},{"key":"2022091418400828500_CIT0026","doi-asserted-by":"crossref","first-page":"323","DOI":"10.4081\/jear.2016.5883","article-title":"Physical characteristics and reproductive performance in Aedes (Diptera: Culicidae)","volume":"48","author":"Dieng","year":"2016","journal-title":"J. Entomol. Acarol. Res"},{"key":"2022091418400828500_CIT0027","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0011790","article-title":"Indoor-breeding of Aedes albopictus in Northern peninsular Malaysia and its potential epidemiological implications","volume":"5","author":"Dieng","year":"2010","journal-title":"PLoS One"},{"key":"2022091418400828500_CIT0028","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/mec.14988","article-title":"The future of parentage analysis: From microsatellites to SNPs and beyond","volume":"28","author":"Flanagan","year":"2019","journal-title":"Mol. Ecol"},{"key":"2022091418400828500_CIT0029","first-page":"586","article-title":"Sexual receptivity in female Aedes aegypti","volume":"28","author":"Gwadz","year":"1968","journal-title":"Mosq. News"},{"key":"2022091418400828500_CIT0030","doi-asserted-by":"crossref","first-page":"201","DOI":"10.2307\/1540069","article-title":"Female sexual behavior as the mechanism rendering Aedes aegypti refractory to insemination","volume":"140","author":"Gwadz","year":"1971","journal-title":"Biol. Bull"},{"key":"2022091418400828500_CIT0031","first-page":"88","article-title":"Reproductive isolation between Florida strains of Aedes aegypti and Aedes albopictus","volume":"10","author":"Harper","year":"1994","journal-title":"J. Am. Mosq. Control Assoc"},{"key":"2022091418400828500_CIT0032","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nbt.2350","article-title":"Successful suppression of a field mosquito population by sustained release of engineered male mosquitoes","volume":"30","author":"Harris","year":"2012","journal-title":"Nat. Biotechnol"},{"key":"2022091418400828500_CIT0033","doi-asserted-by":"crossref","first-page":"15800","DOI":"10.1002\/ece3.8250","article-title":"Waste not, want not: microsatellites remain an economical and informative technology for conservation genetics","volume":"11","author":"Hauser","year":"2021","journal-title":"Ecol. Evol"},{"key":"2022091418400828500_CIT0034","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1603\/ME10071","article-title":"Male mating history and body size influence female fecundity and longevity of the dengue vector Aedes aegypti","volume":"48","author":"Helinski","year":"2011","journal-title":"J. Med. Entomol"},{"key":"2022091418400828500_CIT0035","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/j.jinsphys.2012.07.003","article-title":"Duration and dose-dependency of female sexual receptivity responses to seminal fluid proteins in Aedes albopictus and Ae. aegypti mosquitoes","volume":"58","author":"Helinski","year":"2012","journal-title":"J. Insect Physiol"},{"key":"2022091418400828500_CIT0036","doi-asserted-by":"crossref","first-page":"635","DOI":"10.4269\/ajtmh.2012.11-0225","article-title":"Evidence of polyandry for Aedes aegypti in semifield enclosures","volume":"86","author":"Helinski","year":"2012","journal-title":"Am. J. Trop. Med. Hyg"},{"key":"2022091418400828500_CIT0037","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1073\/pnas.0305511101","article-title":"Assessing fitness costs for transgenic Aedes aegypti expressing the GFP marker","volume":"101","author":"Irvin","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"2022091418400828500_CIT0038","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/j.1365-2052.1997.00186.x","article-title":"Comparisons of three probability formulae for parentage exclusion","volume":"28","author":"Jamieson","year":"1997","journal-title":"Anim. Genet"},{"key":"2022091418400828500_CIT0039","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1017\/S0006323199005423","article-title":"Why do females mate multiply? A review of the genetic benefits","volume":"75","author":"Jennions","year":"2000","journal-title":"Biol. Rev"},{"key":"2022091418400828500_CIT0040","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/242343a0","article-title":"Are mosquitoes monogamous?","volume":"242","author":"Jones","year":"1973","journal-title":"Nature"},{"key":"2022091418400828500_CIT0041","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1111\/j.1755-0998.2009.02787.x","article-title":"COLONY: a program for parentage and sibship inference from multilocus genotype data","volume":"10","author":"Jones","year":"2010","journal-title":"Mol. Ecol. Resour"},{"key":"2022091418400828500_CIT0042","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/j.1755-0998.2009.02778.x","article-title":"A practical guide to methods of parentage analysis","volume":"10","author":"Jones","year":"2010","journal-title":"Mol. Ecol. Resour"},{"key":"2022091418400828500_CIT0043","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1111\/j.1365-294X.2007.03089.x","article-title":"Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment","volume":"16","author":"Kalinowski","year":"2007","journal-title":"Mol. Ecol"},{"key":"2022091418400828500_CIT0044","doi-asserted-by":"crossref","first-page":"e42771","DOI":"10.1371\/journal.pone.0042771","article-title":"Open field release of genetically engineered sterile male Aedes aegypti in Malaysia","volume":"7","author":"Lacroix","year":"2012","journal-title":"PLoS One"},{"key":"2022091418400828500_CIT0045","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.trstmh.2008.02.006","article-title":"Optimization of a semi-nested multiplex PCR to identify Plasmodium parasites in wild-caught Anopheles in Bolivia","volume":"102","author":"Lardeux","year":"2008","journal-title":"Trans. R. Soc. Trop. Med. Hyg"},{"key":"2022091418400828500_CIT0046","author":"Lewis","year":"2001"},{"key":"2022091418400828500_CIT0047","doi-asserted-by":"crossref","first-page":"313","DOI":"10.2987\/moco-31-04-313-320.1","article-title":"Interspecific cross-mating between Aedes aegypti and Aedes albopictus laboratory strains: implication of population density on mating behaviors","volume":"31","author":"Marcela","year":"2015","journal-title":"J. Am. Mosq. Control Assoc"},{"key":"2022091418400828500_CIT0048","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1046\/j.1365-294x.1998.00374.x","article-title":"Statistical confidence for likelihood-based paternity inference in natural populations","volume":"7","author":"Marshall","year":"1998","journal-title":"Mol. Ecol"},{"key":"2022091418400828500_CIT0049","doi-asserted-by":"crossref","first-page":"e62711","DOI":"10.1371\/journal.pone.0062711","article-title":"Fitness of transgenic mosquito Aedes aegypti males carrying a dominant lethal genetic system","volume":"8","author":"Massonnet-Bruneel","year":"2013","journal-title":"PLoS One"},{"key":"2022091418400828500_CIT0050","doi-asserted-by":"crossref","first-page":"6238","DOI":"10.1073\/pnas.1810092116","article-title":"Polyandrous bee provides extended offspring care biparentally as an alternative to monandry based eusociality","volume":"116","author":"Mik\u00e1t","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"2022091418400828500_CIT0051","doi-asserted-by":"crossref","first-page":"4111","DOI":"10.1111\/j.1365-294X.2005.02708.x","article-title":"The potential costs of accounting for genotypic errors in molecular parentage analyses","volume":"14","author":"Morrissey","year":"2005","journal-title":"Mol. Ecol"},{"key":"2022091418400828500_CIT0052","article-title":"Impact of vector control on effective population sizes; empirical evidence for a control-based genetic bottleneck in the tsetse fly Glossina fuscipes","author":"Muhwezi","year":"2020","journal-title":"BioRxiv"},{"key":"2022091418400828500_CIT0053","first-page":"416","article-title":"Interspecific mating between Louisiana strains of Aedes albopictus and Aedes aegypti in the field and laboratory","volume":"5","author":"Nasci","year":"1989","journal-title":"J. Am. Mosq. Control Assoc"},{"key":"2022091418400828500_CIT0054","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.4081\/gh.2021.1008","article-title":"Detecting dengue outbreaks in Malaysia using geospatial techniques","volume":"16","author":"Nellis","year":"2021","journal-title":"Geospat. Health"},{"key":"2022091418400828500_CIT0055","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3390\/insects12030191","article-title":"Sterile insect technique (SIT) against Aedes species mosquitoes: a roadmap and good practice framework for designing, implementing and evaluating pilot field trials","volume":"12","author":"Oliva","year":"2021","journal-title":"Insects"},{"key":"2022091418400828500_CIT0056","doi-asserted-by":"crossref","first-page":"e49414","DOI":"10.1371\/journal.pone.0049414","article-title":"The sterile insect technique for controlling populations of Aedes albopictus (Diptera: Culicidae) on Reunion Island: mating vigor of sterilized males","volume":"7","author":"Oliva","year":"2012","journal-title":"PLoS One"},{"key":"2022091418400828500_CIT0057","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1111\/j.1471-8286.2004.00684.x","article-title":"MicroChecker: software for identifying and correcting genotyping errors in microsatellite data","volume":"4","author":"van Oosterhout","year":"2004","journal-title":"Mol. Ecol. Notes"},{"key":"2022091418400828500_CIT0058","doi-asserted-by":"crossref","first-page":"477","DOI":"10.13156\/arac.2020.18.5.477","article-title":"Characterization of four hypervariable microsatellite loci in a nuptial gift-giving spider and its prospect for paternity analyses","volume":"18","author":"Pandulli-Alonso","year":"2020","journal-title":"Arachnology"},{"key":"2022091418400828500_CIT0059","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1146\/annurev.ento.50.071803.130439","article-title":"Mosquito behavior and vector control","volume":"50","author":"Pates","year":"2005","journal-title":"Annu. Rev. Entomol"},{"key":"2022091418400828500_CIT0060","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1603\/0022-2585(2007)44[422:AABSIM]2.0.CO;2","article-title":"Age and body size influence male sperm capacity of the dengue vector Aedes aegypti (Diptera: Culicidae)","volume":"44","author":"Ponlawat","year":"2007","journal-title":"J. Med. Entomol"},{"key":"2022091418400828500_CIT0061","doi-asserted-by":"crossref","first-page":"20190591","DOI":"10.1098\/rspb.2019.0591","article-title":"Male competition and the evolution of mating and life-history traits in experimental populations of Aedes aegypti","volume":"286","author":"Qureshi","year":"2019","journal-title":"Proc. R. Soc. B Biol. Sci"},{"key":"2022091418400828500_CIT0062","doi-asserted-by":"crossref","first-page":"e0008492","DOI":"10.1371\/journal.pntd.0008492","article-title":"Insecticide resistance and genetic structure of Aedes aegypti populations from Rio de Janeiro State, Brazil","volume":"15","author":"Rahman","year":"2021","journal-title":"PLoS Negl. Trop. Dis"},{"key":"2022091418400828500_CIT0063","doi-asserted-by":"crossref","first-page":"e0009205","DOI":"10.1371\/journal.pntd.0009205","article-title":"Insecticide resistance in dengue vectors from hotspots in Selangor, Malaysia","volume":"15","author":"Rasli","year":"2021","journal-title":"PLoS Negl. Trop. Dis"},{"key":"2022091418400828500_CIT0064","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/j.1469-185X.1988.tb00669.x","article-title":"Mating frequency and fecundity in insects","volume":"63","author":"Ridley","year":"1988","journal-title":"Biol. Rev"},{"key":"2022091418400828500_CIT0065","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4269\/ajtmh.14-0718","article-title":"Evidence of limited polyandry in a natural population of Aedes aegypti","volume":"93","author":"Richardson","year":"2015","journal-title":"Am. J. Trop. Med. Hyg"},{"key":"2022091418400828500_CIT0066","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1146\/annurev.en.41.010196.001545","article-title":"Geographic structure of insect populations: gene flow, phylogeography, and their uses","volume":"41","author":"Roderick","year":"1996","journal-title":"Annu. Rev. Entomol"},{"key":"2022091418400828500_CIT0067","volume-title":"Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with dengue virus transmission","author":"Rueda","year":"2004"},{"key":"2022091418400828500_CIT0068","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0030919","article-title":"Changing domesticity of Aedes aegypti in northern peninsular Malaysia: reproductive consequences and potential epidemiological implications","volume":"7","author":"Saifur","year":"2012","journal-title":"PLoS One"},{"key":"2022091418400828500_CIT0069","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/j.1471-8286.2006.01533.x","article-title":"Polymorphic microsatellite markers for studies of Aedes aegypti (Diptera: Culicidae), the vector of dengue and yellow fever","volume":"7","author":"Slotman","year":"2006","journal-title":"Mol. Ecol. Notes"},{"key":"2022091418400828500_CIT0070","doi-asserted-by":"crossref","first-page":"404","DOI":"10.2307\/1539644","article-title":"Seminal loss in repeatedly mated female Aedes aegypti","volume":"132","author":"Spielman","year":"1967","journal-title":"Biol. Bull"},{"key":"2022091418400828500_CIT0071","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3390\/insects10100317","article-title":"Use of DNA markers for grape phylloxera population and evolutionary genetics: From RAPDs to SSRs and beyond","volume":"10","author":"Tello","year":"2019","journal-title":"Insects"},{"key":"2022091418400828500_CIT0072","first-page":"226","article-title":"Hybridization between Aedes aegypti and Aedes albopictus in Malaysia","volume":"4","author":"Thomas","year":"1973","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"2022091418400828500_CIT0073","doi-asserted-by":"crossref","first-page":"4772","DOI":"10.1073\/pnas.1019295108","article-title":"Genetic elimination of dengue vector mosquitoes","volume":"108","author":"de Valdez","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"2022091418400828500_CIT0074","doi-asserted-by":"crossref","first-page":"5061","DOI":"10.1111\/j.1365-294X.2010.04865.x","article-title":"Effects of genotyping errors on parentage exclusion analysis","volume":"19","author":"Wang","year":"2010","journal-title":"Mol. Ecol"},{"key":"2022091418400828500_CIT0075","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1603\/ME11152","article-title":"Microsatellite-based parentage analysis of Aedes aegypti (Diptera: Culicidae) using non-lethal DNA sampling","volume":"49","author":"Wong","year":"2012","journal-title":"J. Med. Entomol"},{"key":"2022091418400828500_CIT0076","author":"World Health Organization","year":"2009"},{"key":"2022091418400828500_CIT0077","author":"World Health Organization","year":"2022"}],"container-title":["Journal of Medical Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jme\/article-pdf\/59\/5\/1525\/45849818\/tjac081.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jme\/article-pdf\/59\/5\/1525\/45849818\/tjac081.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T16:16:03Z","timestamp":1700756163000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jme\/article\/59\/5\/1525\/6613457"}},"subtitle":[],"editor":[{"given":"David","family":"Severson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,6,23]]},"references-count":77,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,6,23]]},"published-print":{"date-parts":[[2022,9,14]]}},"URL":"https:\/\/doi.org\/10.1093\/jme\/tjac081","relation":{},"ISSN":["0022-2585","1938-2928"],"issn-type":[{"value":"0022-2585","type":"print"},{"value":"1938-2928","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,9,1]]},"published":{"date-parts":[[2022,6,23]]}}}