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Among the most effective strategies for the prevention of vector-borne diseases are those aimed at reducing human\u2013vector interactions, such as insecticide applications and insecticide-treated bed nets (ITNs). In some areas where ITNs are widely used, behavioral adaptations have resulted in mosquitoes shifting their time of blood feeding to earlier or later in the night when the bed nets are not being employed. Little is known about the genetic basis of these behavioral shifts. We conducted quantitative trait locus (QTL) analysis using two strains of Culex pipiens sensu lato with contrasting blood feeding behaviors, wherein the lab adapted Shasta strain blood feeds at any time of the day or night, while the newly established Trinidad strain feeds only at night. We identified a single locus on chromosome 2 associated with the observed variation in feeding times. None of the core clock genes period, timeless, clock, cycle, PAR-domain protein 1, vrille, discs overgrown, cryptochrome 1, or cryptochrome 2 were located within the QTL region. We then monitored locomotor behavior to determine if they differed in their flight activity. The highly nocturnal Trinidad strain showed little daytime activity while the day-feeding Shasta strain was active during the day, suggesting blood feeding behavior and flight activity are physiologically linked.<\/jats:p>","DOI":"10.1093\/jme\/tjac118","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T17:40:36Z","timestamp":1660758036000},"page":"1500-1506","source":"Crossref","is-referenced-by-count":7,"title":["Quantitative Trait Locus Determining the Time of Blood Feeding in <i>Culex pipiens<\/i> (Diptera: Culicidae)"],"prefix":"10.1093","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2350-0625","authenticated-orcid":false,"given":"Paul V","family":"Hickner","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Notre Dame , Notre Dame, IN 46556 , USA"},{"name":"USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory , Kerrville, TX 78028 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Notre Dame , Notre Dame, IN 46556 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel S C","family":"Rund","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Notre Dame , Notre Dame, IN 46556 , USA"},{"name":"Center for Research Computing, University of Notre Dame , Notre Dame, IN 46556 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5798-3365","authenticated-orcid":false,"given":"David W","family":"Severson","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Notre Dame , Notre Dame, IN 46556 , USA"},{"name":"Department of Life Sciences, University of the West Indies , Saint Augustine, Trinidad, West Indies"},{"name":"Department of Medical and Molecular Genetics, Indiana University School of Medicine , South Bend, IN , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,8,17]]},"reference":[{"key":"2023081701162624100_CIT0001","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nn1007","article-title":"A role for CK2 in the Drosophila circadian oscillator","volume":"6","author":"Akten","year":"2003","journal-title":"Nat. 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