{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T12:29:35Z","timestamp":1754483375178,"version":"3.37.3"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"1","funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["3020-32000-019-00D","3020-32000-020-00D","3020-32000-018-00D"],"award-info":[{"award-number":["3020-32000-019-00D","3020-32000-020-00D","3020-32000-018-00D"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007917","name":"Agricultural Research Service","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007917","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Bio and Agro-Defense Facility Transition Fund"},{"DOI":"10.13039\/100006088","name":"National Center for Emerging and Zoonotic Infectious Diseases","doi-asserted-by":"publisher","award":["P20GM130448"],"award-info":[{"award-number":["P20GM130448"]}],"id":[{"id":"10.13039\/100006088","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Center of Excellence for Influenza Research and Response","award":["75N93021C00016"],"award-info":[{"award-number":["75N93021C00016"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A cell line was established from Culex tarsalis Coquillett embryonated eggs and designated as CxTr. The cell line is heterogeneous, composed predominantly of small, round cells, and spindle-shaped cells with a doubling time of approximately 52\u201360 h. The identity of the cell line was verified as Cx. tarsalis by sequencing of cytochrome oxidase I and the cells were found to be free of contaminating cells, bacteria, fungi, and mycoplasma. The permissiveness of CxTr cells to arbovirus infection was investigated with vaccine and wildtype arboviruses from four viral families: Flaviviridae (Japanese encephalitis virus), Phenuiviridae (Rift Valley fever phlebovirus), Rhabdoviridae (vesicular stomatitis virus), and Togaviridae (Mayaro virus). All viruses were able to infect and replicate within CxTr cells.<\/jats:p>","DOI":"10.1093\/jme\/tjac155","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T15:01:20Z","timestamp":1666191680000},"page":"239-244","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of a <i>Culex tarsalis<\/i> (Diptera: Culicidae) Cell Line and its Permissiveness to Arbovirus Infection"],"prefix":"10.1093","volume":"60","author":[{"given":"Erin E","family":"Schirtzinger","sequence":"first","affiliation":[{"name":"Arthropod-borne Animal Diseases Research Unit, USDA, ARS , Manhattan, KS , USA"},{"name":"Department of Diagnostic Medicine\/Pathology, College of Veterinary Medicine, Kansas State University , Manhattan, KS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dane C","family":"Jasperson","sequence":"additional","affiliation":[{"name":"Arthropod-borne Animal Diseases Research Unit, USDA, ARS , Manhattan, KS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dustin A","family":"Swanson","sequence":"additional","affiliation":[{"name":"Arthropod-borne Animal Diseases Research Unit, USDA, ARS , Manhattan, KS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana","family":"Mitzel","sequence":"additional","affiliation":[{"name":"Foreign Arthropod-borne Animal Diseases Research Unit, National Bio- and Agro-Defense Facility, USDA, ARS , Manhattan, KS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara S","family":"Drolet","sequence":"additional","affiliation":[{"name":"Arthropod-borne Animal Diseases Research Unit, USDA, ARS , Manhattan, KS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juergen A","family":"Richt","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine\/Pathology, College of Veterinary Medicine, Kansas State University , Manhattan, KS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7877-9224","authenticated-orcid":false,"given":"William C","family":"Wilson","sequence":"additional","affiliation":[{"name":"Foreign Arthropod-borne Animal Diseases Research Unit, National Bio- and Agro-Defense Facility, USDA, ARS , Manhattan, KS , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"key":"2023052322371788700_CIT0001","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1111\/jeb.12939","article-title":"Rise and fall of vector infectivity during sequential strain displacements by mosquito-borne dengue virus","volume":"29","author":"Andrade","year":"2016","journal-title":"J. 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