{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:01:40Z","timestamp":1790316100664,"version":"4.1.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T00:00:00Z","timestamp":1676592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21 AI146513-01A1"],"award-info":[{"award-number":["R21 AI146513-01A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The cosmopolitan mosquito Aedes aegypti is a vector of harmful arboviruses. Pyrethroid insecticides are used to reduce adult populations and prevent the spread of disease. Pyrethroids target the insect voltage-gated sodium channel (VGSC). Collectively, mutations in Vgsc that confer resistance are referred to as knock-down resistance or kdr. There are numerous kdr mutations found in A. aegypti Vgsc, and there is co-occurrence of some mutations. Full-length cDNA sequences have identified nine known kdr (e.g., 1534C) alleles. The 1534C allele is among the most common kdr alleles, but allele frequencies between populations vary considerably. We used the 1534C:RK strain, which has the 1534C (kdr) allele in the genetic background of the insecticide susceptible Rockefeller (ROCK) strain, and conducted population cage experiments to assess the potential intrinsic fitness cost of the 1534C allele relative to the susceptible allele (F1534) in the ROCK strain. Individuals were genotyped across generations using allele specific PCR. A fitness cost of the 1534C allele was detected across seven generations of mosquitos reared in the absence of insecticide selection pressure. The decrease in allele frequency was not due to drift. Comparison of our results to previous studies suggests that the magnitude of the fitness cost of kdr alleles in the absence of insecticide is disconnected from the level of resistance they confer, and that the fitness costs of different kdr alleles can be variable.<\/jats:p>","DOI":"10.1093\/jme\/tjad015","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T11:12:06Z","timestamp":1676632326000},"page":"494-499","source":"Crossref","is-referenced-by-count":13,"title":["A Globally Distributed Insecticide Resistance Allele Confers a Fitness Cost in the Absence of Insecticide in <i>Aedes aegypti<\/i> (Diptera: Culicidae), the Yellow Fever Mosquito"],"prefix":"10.1093","volume":"60","author":[{"given":"Cera R","family":"Fisher","sequence":"first","affiliation":[{"name":"Department of Entomology, Comstock Hall, Cornell University , Ithaca, NY , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0990-5856","authenticated-orcid":false,"given":"Anastacia E","family":"Dressel","sequence":"additional","affiliation":[{"name":"Department of Entomology, Comstock Hall, Cornell University , Ithaca, NY , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan J","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Entomology, Comstock Hall, Cornell University , Ithaca, NY , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0765-1896","authenticated-orcid":false,"given":"Jeffrey G","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Entomology, Comstock Hall, Cornell University , Ithaca, NY , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,2,17]]},"reference":[{"key":"2023051216170683700_CIT0001","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1016\/S0140-6736(17)31450-2","article-title":"An update on Zika virus infection","volume":"390","author":"Baud","year":"2017","journal-title":"Lancet."},{"key":"2023051216170683700_CIT0002","doi-asserted-by":"crossref","first-page":"e60878","DOI":"10.1371\/journal.pone.0060878","article-title":"Assessing the effects of Aedes aegypti kdr mutations on pyrethroid resistance and its fitness cost","volume":"8","author":"Brito","year":"2013","journal-title":"PLoS One."},{"key":"2023051216170683700_CIT0003","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.ibmb.2009.01.001","article-title":"A novel amino acid substitution in a voltage-gated sodium channel is associated with knockdown resistance to permethrin in Aedes aegypti","volume":"39","author":"Chang","year":"2009","journal-title":"Insect Biochem. 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