{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:32:44Z","timestamp":1790422364278,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The yellow fever mosquito, Aedes aegypti L., can transmit several pathogens responsible for human diseases. With insecticide resistance development becoming a concern, alternative control strategies are needed for Ae. aegypti. Sterile insect technique (SIT) is an increasingly popular option being explored. However, logistical issues in mass production and sterilization make it difficult to maintain a SIT program. Male mosquitoes are typically irradiated as pupae because this is the earliest developmental point at which females can be separated from males, but asynchrony in pupation and high variability in pupal responses to irradiation based on pupal age make it difficult to sterilize mass quantities of pupae on a regular schedule in a rearing facility. Young adult mosquitoes have wider windows for irradiation sterilization than pupae, which can allow facilities to have fixed schedules for irradiation. We produced a workflow for adult Ae. aegypti irradiation in a mosquito control district with an operational SIT program that currently irradiates pupae. The impacts of chilling, compaction, and radiation dose on survival were all assessed before combining them into a complete adult irradiation protocol. Males chilled up to 16 h prior to compaction and compacted to 100 males\/cm3 during radiation resulted in low mortality. Males irradiated as adults had increased longevity and similar sterility compared to males irradiated as pupae. Additionally, males sterilized as adults were more sexually competitive than males sterilized as pupae. Thus, we have shown that irradiating adult males can be a viable option to increase the efficiency of this operational mosquito SIT program.<\/jats:p>","DOI":"10.1093\/jme\/tjad079","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T11:14:51Z","timestamp":1687346091000},"page":"1038-1047","source":"Crossref","is-referenced-by-count":22,"title":["Developing methods for chilling, compacting, and sterilizing adult <i>Aedes aegypti<\/i> (Diptera: Culicidae) and comparing mating competitiveness between males sterilized as adults versus pupae for sterile male release"],"prefix":"10.1093","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6478-608X","authenticated-orcid":false,"given":"Dylan A","family":"Tussey","sequence":"first","affiliation":[{"name":"Department of Entomology & Nematology, University of Florida , Gainesville, FL , USA"},{"name":"Department of Entomology, University of California Riverside , Parlier, CA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9912-7746","authenticated-orcid":false,"given":"Rachel","family":"Morreale","sequence":"additional","affiliation":[{"name":"Lee County Mosquito Control District , Lehigh Acres, FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8168-4050","authenticated-orcid":false,"given":"Danilo O","family":"Carvalho","sequence":"additional","affiliation":[{"name":"International Atomic Energy Agency, Insect Pest Control Section , Siebersdorf , Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Stenhouse","sequence":"additional","affiliation":[{"name":"Lee County Mosquito Control District , Lehigh Acres, FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron M","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Lee County Mosquito Control District , Lehigh Acres, FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0912-6497","authenticated-orcid":false,"given":"David F","family":"Hoel","sequence":"additional","affiliation":[{"name":"Lee County Mosquito Control District , Lehigh Acres, FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A","family":"Hahn","sequence":"additional","affiliation":[{"name":"Department of Entomology & Nematology, University of Florida , Gainesville, FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,6,21]]},"reference":[{"issue":"10","key":"2023091211184755700_CIT0001","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/S0965-1748(02)00087-5","article-title":"Re-engineering the sterile insect technique","volume":"32","author":"Alphey","year":"2002","journal-title":"Insect Biochem Mol Biol"},{"issue":"3","key":"2023091211184755700_CIT0002","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/j.1365-2915.2005.00565.x","article-title":"Optimal life stage for radiation sterilization of Anopheles males and their fitness for release","volume":"19","author":"Andreasen","year":"2005","journal-title":"Med Vet Entomol"},{"key":"2023091211184755700_CIT0003","volume-title":"Best management practices for integrated mosquito management","author":"Association","year":"2021"},{"key":"2023091211184755700_CIT0004","doi-asserted-by":"crossref","first-page":"942654","DOI":"10.3389\/fbioe.2022.942654","article-title":"Response of male adult Aedes mosquitoes to gamma radiation in different nitrogen environments","volume":"10","author":"Bimbil\u00e9 Somda","year":"2022","journal-title":"Front Bioeng Biotechnol"},{"issue":"11","key":"2023091211184755700_CIT0005","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.pt.2020.09.005","article-title":"Yes, irradiated sterile male mosquitoes can be sexually competitive!","volume":"36","author":"Bouyer","year":"2020","journal-title":"Trends Parasitol"},{"issue":"1","key":"2023091211184755700_CIT0006","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1186\/s13071-022-05512-3","article-title":"A sterile insect technique pilot trial on Captiva Island: defining mosquito population parameters for sterile male releases using mark-release-recapture","volume":"15","author":"Carvalho","year":"2022","journal-title":"Parasit Vectors"},{"key":"2023091211184755700_CIT0007","doi-asserted-by":"crossref","first-page":"S91","DOI":"10.1016\/j.actatropica.2013.06.022","article-title":"The diel copulation periodicity of the mosquito, Aedes aegypti (L.) (Diptera: Culicidae) at indoor and outdoor sites in Trinidad, West Indies","volume":"132","author":"Chadee","year":"2014","journal-title":"Acta Trop"},{"key":"2023091211184755700_CIT0008","volume-title":"Aedes aegypti (L.) the yellow fever mosquito: its life history, bionomics and structure","author":"Christophers","year":"1960","edition":"1"},{"issue":"6","key":"2023091211184755700_CIT0009","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jisesa\/iey103","article-title":"Toward implementation of mosquito sterile insect technique: the effect of storage conditions on survival of male Aedes aegypti mosquitoes (Diptera: Culicidae) during transport","volume":"18","author":"Chung","year":"2018","journal-title":"J Insect Sci"},{"issue":"8","key":"2023091211184755700_CIT0010","doi-asserted-by":"crossref","first-page":"e0221822","DOI":"10.1371\/journal.pone.0221822","article-title":"Investigating the impact of chilling temperature on male Aedes aegypti and Aedes albopictus survival","volume":"14","author":"Culbert","year":"2019","journal-title":"PLoS One"},{"key":"2023091211184755700_CIT0011","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1186\/s13071-018-3191-z","article-title":"Longevity of mass-reared male Anopheles arabiensis and Aedes aegypti under simulated environmental field conditions","volume":"11","author":"Culbert","year":"2018","journal-title":"Parasit Vectors"},{"key":"2023091211184755700_CIT0012","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1475-2875-8-S2-S2","article-title":"Historical applications of induced sterilization in field populations of mosquitoes","volume":"8","author":"Dame","year":"2009","journal-title":"Malar J"},{"key":"2023091211184755700_CIT0013","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/1-4020-4051-2_11","article-title":"Sterile insect supply, emergence, and release","volume-title":"Sterile Insect Tech. 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