{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:11:56Z","timestamp":1740147116234,"version":"3.37.3"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T00:00:00Z","timestamp":1693958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Education, Malaysia","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Higher Institution Centre of Excellence","award":["MO002-2019"],"award-info":[{"award-number":["MO002-2019"]}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N42A660464"],"award-info":[{"award-number":["N42A660464"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002842","name":"Chiang Mai University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002842","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Simulium (Asiosimulium) khongchiamense sp. nov. is described based on females, males, pupae, and mature larvae collected from Khong Chiam District, Ubon Ratchathani Province, northeastern Thailand. It is characterized in the female by the medium-long sensory vesicle, scutum with 3 dark longitudinal vittae and elongate cercus; in the male by the number of upper-eye (large) facets in 17 or 18 vertical rows and 18 or 19 horizontal rows, hind basitarsus moderately enlarged and ventral plate with the posterior margin moderately concave medially; in the pupa by the head and thoracic integument sparsely covered with tubercles and gill of arborescent type with 32 or 33 filaments; and in the larva by the postgenal cleft deep, reaching the posterior margin of the hypostoma and sheath of the subesophageal ganglion dark pigmented. DNA analysis based on COI gene of all known species of the subgenus Asiosimulium, except for S. shanense and S. suchitrae, indicated that this new species can be clearly differentiated from all other related species (S. phurueaense, S. oblongum, S. saeungae, S. furvum, and S. wanchaii) with interspecific genetic distances ranging between 4.79% and 19.18%. This is the eighth species of the subgenus Asiosimulium. Taxonomic notes are given to distinguish this new species from the 7 known species members in its same subgenus. Additionally, keys to species of all members in the subgenus Asiosimulium are provided.<\/jats:p>","DOI":"10.1093\/jme\/tjad118","type":"journal-article","created":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T00:21:40Z","timestamp":1693959700000},"page":"1330-1342","source":"Crossref","is-referenced-by-count":3,"title":["A new species of <i>Simulium<\/i> (<i>Asiosimulium<\/i>) (Diptera: Simuliidae) from northeastern Thailand, with its phylogenetic relationships with related species in the subgenus <i>Asiosimulium<\/i>"],"prefix":"10.1093","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5716-4725","authenticated-orcid":false,"given":"Kittipat","family":"Aupalee","sequence":"first","affiliation":[{"name":"Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University , Chiang Mai 50200 , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wichai","family":"Srisuka","sequence":"additional","affiliation":[{"name":"Entomology Section, Queen Sirikit Botanic Garden , P.O. Box 7, Maerim, Chiang Mai 50180 , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kritsana","family":"Taai","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Western University , Kanchanaburi 71170 , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Takaoka","sequence":"additional","affiliation":[{"name":"Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Centre of Excellence (HICoE), Universiti Malaya , Kuala Lumpur 50603 , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3550-5992","authenticated-orcid":false,"given":"Atiporn","family":"Saeung","sequence":"additional","affiliation":[{"name":"Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University , Chiang Mai 50200 , Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,9,6]]},"reference":[{"volume-title":"The black flies (Simuliidae) of North America","year":"2004","author":"Adler","key":"2023111418560502100_CIT0002"},{"key":"2023111418560502100_CIT0003","first-page":"772","article-title":"jModelTest 2: more models, new heuristics and parallel computing","volume-title":"Nat Methods","author":"Darriba","year":"2012"},{"issue":"5","key":"2023111418560502100_CIT0004","first-page":"294","article-title":"DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates","volume":"3","author":"Folmer","year":"1994","journal-title":"Mol Mar Biol Biotechnol"},{"key":"2023111418560502100_CIT0005","first-page":"1647","article-title":"Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data","volume-title":"Bioinformatics","author":"Kearse","year":"2012"},{"key":"2023111418560502100_CIT0006","first-page":"111","article-title":"A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences","volume-title":"J Mol Evol","author":"Kimura","year":"1980"},{"key":"2023111418560502100_CIT0007","first-page":"329","article-title":"Phylogenetic analysis of three subgenera: Asiosimulium, Daviesellum and Wallacellum, of the genus Simulium s. l. endemic in the Oriental Region","volume-title":"Med Entomol Zool","author":"Otsuka","year":"2007"},{"key":"2023111418560502100_CIT0008","first-page":"345","article-title":"Trait-based and phylogenetic community ecology of black flies (Diptera: Simuliidae) in tropical streams of Thailand","volume-title":"Hydrobiologia","author":"Pangjanda","year":"2016"},{"key":"2023111418560502100_CIT0009","first-page":"262","article-title":"DNA barcoding of tropical black flies (Diptera: Simuliidae) of Thailand","volume-title":"Mol Ecol Resour","author":"Pramual","year":"2014"},{"key":"2023111418560502100_CIT0010","first-page":"106116","article-title":"DNA barcoding of tropical black flies (Diptera: Simuliidae) in Thailand: one decade of progress","volume-title":"Acta Trop","author":"Pramual","year":"2021"},{"key":"2023111418560502100_CIT0011","first-page":"539","article-title":"MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space","volume-title":"Syst Biol","author":"Ronquist","year":"2012"},{"key":"2023111418560502100_CIT0012","first-page":"106790","article-title":"Discovery of a new black fly species in the Simulium (Simulium) striatum species-group (Diptera: Simuliidae) from northeastern Thailand and its phylogenetic relationships with seven Thai related species","volume-title":"Acta Trop","author":"Srisuka","year":"2023"},{"key":"2023111418560502100_CIT0013","first-page":"106947","article-title":"Simulium (Gomphostilbia) wijiti, a new species of black fly (Diptera: Simuliidae) from northern Thailand and its genetic position with related species in the Simulium ceylonicum species-group","volume-title":"Acta Trop","author":"Srisuka","year":"2023"},{"key":"2023111418560502100_CIT0014","first-page":"574","article-title":"Biodiversity, seasonal abundance, and distribution of blackflies (Diptera: Simuliidae) in six different regions of Thailand","volume-title":"Parasit Vectors","author":"Srisuka","year":"2017"},{"issue":"3","key":"2023111418560502100_CIT0015","first-page":"504","article-title":"Description of male, pupa and larva of Simulium (Asiosimulium) wanchaii (Diptera: Simuliidae) from Thailand, with keys to identify four species of the subgenus Asiosimulium","volume":"32","author":"Srisuka","year":"2015","journal-title":"Trop Biomed"},{"key":"2023111418560502100_CIT0016","first-page":"1237","article-title":"Molecular Evolutionary Genetics Analysis (MEGA) for macOS","volume-title":"Mol Biol Evol","author":"Stecher","year":"2020"},{"volume-title":"The black flies (Diptera: Simuliidae) of Sulawesi, Maluku and Irian Jaya","year":"2003","author":"Takaoka","key":"2023111418560502100_CIT0017"},{"key":"2023111418560502100_CIT0018","first-page":"1","article-title":"Morphotaxonomic revision of Simulium (Gomphostilbia) (Diptera: Simuliidae) in the Oriental Region","volume-title":"Zootaxa","author":"Takaoka","year":"2012"},{"volume-title":"Black flies and parasitic diseases: a handbook for those who will begin to study black flies","year":"2016","author":"Takaoka","key":"2023111418560502100_CIT0019"},{"key":"2023111418560502100_CIT0020","first-page":"43","article-title":"A new species of Simulium (Simulium) (Diptera: Simuliidae) from Thailand","volume-title":"Med Entomol Zool","author":"Takaoka","year":"2005"},{"key":"2023111418560502100_CIT0021","first-page":"45","article-title":"A new species of the subgenus Simulium (Asiosimulium) (Diptera: Simuliidae) from Thailand","volume-title":"Med Entomol Zool","author":"Takaoka","year":"2006"},{"key":"2023111418560502100_CIT0023","first-page":"1","article-title":"New species of black flies (Diptera: Simuliidae) from Nepal","volume-title":"Zootaxa","author":"Takaoka","year":"2010"},{"key":"2023111418560502100_CIT0024","first-page":"137","article-title":"A new species of Simulium (Asiosimulium) (Diptera: Simuliidae) from Myanmar","volume-title":"Med Entomol Zool","author":"Takaoka","year":"2017"},{"key":"2023111418560502100_CIT0025","first-page":"569","article-title":"A new species of Simulium (Asiosimulium) (Diptera: Simuliidae) from Thailand","volume-title":"J Med Entomol","author":"Takaoka","year":"2018"},{"key":"2023111418560502100_CIT0026","first-page":"53","article-title":"Checklist and keys for the black flies (Diptera: Simuliidae) of Thailand","volume-title":"Med Entomol Zool","author":"Takaoka","year":"2019"},{"key":"2023111418560502100_CIT0027","first-page":"493","article-title":"Simulium (Asiosimulium) furvum, a new species of black fly (Diptera: Simuliidae) from Thailand","volume-title":"J Med Entomol","author":"Takaoka","year":"2013"},{"key":"2023111418560502100_CIT0028","first-page":"111","article-title":"A new black fly (Diptera: Simuliidae) species of the subgenus Asiosimulium Takaoka & Choochote from Thailand","volume-title":"Zootaxa","author":"Tangkawanit","year":"2018"}],"container-title":["Journal of Medical Entomology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jme\/article-pdf\/60\/6\/1330\/53404844\/tjad118.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jme\/article-pdf\/60\/6\/1330\/53404844\/tjad118.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T18:57:15Z","timestamp":1699988235000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jme\/article\/60\/6\/1330\/7260695"}},"subtitle":[],"editor":[{"given":"Richard","family":"Wilkerson","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,9,6]]},"references-count":26,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,9,6]]},"published-print":{"date-parts":[[2023,11,14]]}},"URL":"https:\/\/doi.org\/10.1093\/jme\/tjad118","relation":{},"ISSN":["0022-2585","1938-2928"],"issn-type":[{"type":"print","value":"0022-2585"},{"type":"electronic","value":"1938-2928"}],"subject":[],"published-other":{"date-parts":[[2023,11,1]]},"published":{"date-parts":[[2023,9,6]]}}}