{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T09:59:03Z","timestamp":1768903143559,"version":"3.49.0"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A previous laboratory study using Haemaphysalis longicornis Neumann (Acari: Ixodidae) ticks of North American origin showed that larvae could acquire the Lyme disease spirochete, Borrelia burgdorferi sensu stricto (s.s.) (Spirochaetales: Spirochaetaceae) while feeding to completion on infected mice. However, the infection was lost during the molt to the nymphal stage. Nonetheless, questing H. longicornis nymphs and adults collected by drag sampling in the northeastern United States have been reported infected with B. burgdorferi s.s. DNA; occasionally these ticks appeared to be partially engorged. This raises the question of whether H. longicornis ticks can (i) acquire B. burgdorferi s.s. during an interrupted, partial blood meal on an infected host and (ii) transmit spirochetes while completing the blood meal on a second host. In this laboratory study, we demonstrated that H. longicornis nymphs could acquire B. burgdorferi s.s. from infected Mus musculus mice during a partial blood meal. Borrelia burgdorferi s.s. was detected by a multiplex polymerase chain reaction amplicon sequencing assay in 2 of 32 (6.3%) nymphs allowed to remain attached to infected mice for 48 h but, paradoxically, not in any of 25 nymphs that remained attached to infected mice for 72 h. Unfortunately, due to the low percentage of infected nymphs, we were not able to examine if such partially fed, infected nymphs were able to transmit B. burgdorferi s.s. while completing their blood meal on a second, na\u00efve host.<\/jats:p>","DOI":"10.1093\/jme\/tjae156","type":"journal-article","created":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T17:54:03Z","timestamp":1735840443000},"page":"475-478","source":"Crossref","is-referenced-by-count":2,"title":["Acquisition of <i>Borrelia burgdorferi<\/i> sensu stricto (Spirochaetales: Spirochaetaceae) by <i>Haemaphysalis longicornis<\/i> (Acari: Ixodidae) nymphs during interrupted feeding"],"prefix":"10.1093","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3811-1956","authenticated-orcid":false,"given":"Christina M","family":"Parise","sequence":"first","affiliation":[{"name":"Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention , Fort Collins, CO ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9526-1155","authenticated-orcid":false,"given":"Shelby L","family":"Ford","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention , Fort Collins, CO ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8555-1095","authenticated-orcid":false,"given":"James","family":"Burtis","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention , Fort Collins, CO ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3652-2359","authenticated-orcid":false,"given":"Andrias","family":"Hojgaard","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention , Fort Collins, CO ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5942-528X","authenticated-orcid":false,"given":"Rebecca J","family":"Eisen","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention , Fort Collins, CO ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Eisen","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention , Fort Collins, CO ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,1,2]]},"reference":[{"key":"2025031903425299500_CIT0001","doi-asserted-by":"crossref","first-page":"234","DOI":"10.4182\/HKTD2318","article-title":"Weight change dynamics during feeding","volume-title":"A translation of Bloodsucking ticks (Ixodoidea)\u2014vectors of diseases of man and animals","author":"Balashov","year":"1972"},{"key":"2025031903425299500_CIT0002","doi-asserted-by":"publisher","first-page":"101311","DOI":"10.1016\/j.ttbdis.2019.101311","article-title":"Failure of the Asian longhorned tick, Haemaphysalis longicornis, to serve as an experimental vector of the Lyme disease spirochete, Borrelia burgdorferi sensu stricto","volume":"11","author":"Breuner","year":"2020","journal-title":"Ticks Tick Borne Dis"},{"key":"2025031903425299500_CIT0003","doi-asserted-by":"publisher","first-page":"454","DOI":"10.3390\/pathogens11040454","article-title":"Pathogen spillover to an invasive tick species: first detection of Bourbon virus in Haemaphysalis longicornis in the United States","volume":"11","author":"Cumbie","year":"2022","journal-title":"Pathogens"},{"key":"2025031903425299500_CIT0004","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1186\/s13071-021-04659-9","article-title":"Isolate of Theileria orientalis, Ikeda genotype, is transmitted to cattle by the invasive Asian longhorned tick, Haemaphysalis longicornis","volume":"14","author":"Dinkel","year":"2021","journal-title":"Parasit Vectors"},{"key":"2025031903425299500_CIT0005","doi-asserted-by":"publisher","first-page":"1398","DOI":"10.1093\/jme\/tjad119","article-title":"Invasive Haemaphysalis longicornis (Acari: Ixodidae) investigation in South Carolina: new records of establishment, pathogen prevalence, and blood meal analyses","volume":"60","author":"Dye-Braumuller","year":"2023","journal-title":"J. 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