{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T16:55:13Z","timestamp":1781456113691,"version":"3.54.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","award":["N42A660641"],"award-info":[{"award-number":["N42A660641"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Grant Funding from the Ratchadaphiseksomphot Fund, Faculty of Medicine, Chulalongkorn University","award":["RA68\/036"],"award-info":[{"award-number":["RA68\/036"]}]},{"name":"Second Century Fund (C2F), Chulalongkorn University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Culex quinquefasciatus (Say) is a significant vector of lymphatic filariasis and arboviruses. Current control strategies rely heavily on chemical insecticides, highlighting the need for alternative and environmentally sustainable approaches. This study evaluated the effects of the microalga Chlorella sorokiniana KU.B2 on the development of Cx. quinquefasciatus larvae. Larvae were reared on microalgal-rabbit food diets: a control (100% rabbit food) and four treatments with increasing proportions of C. sorokiniana suspension (25 microalgae [Al], 50Al, 75Al, and 100Al). Additional rabbit food-only controls with matched food proportions were included to distinguish microalgal effects from reduced food availability. Larval growth, pupation, adult emergence, and mortality were assessed, and gut histology and ultrastructure were examined descriptively. Higher proportions of microalgae (75Al and 100Al) were associated with failure of pupation and adult emergence, resulting in complete larval mortality. Lower proportions (25Al and 50Al) caused delayed development and reduced survival compared with controls. Larvae provided reduced amounts of rabbit food alone were still able to complete development, indicating that the observed effects were associated with microalgal inclusion rather than reduced food availability alone. Histological and ultrastructural observations revealed numerous intact and partially degraded microalgal cells within the larval gut, while recovery of viable cells after gut passage suggested that some ingested cells remained viable after transit through the larval digestive tract. Overall, C. sorokiniana KU.B2 negatively affected the development and survival of Cx. quinquefasciatus larvae in a dose-dependent manner. Further investigation into the mechanisms underlying these effects and their ecological relevance is needed.<\/jats:p>","DOI":"10.1093\/jme\/tjag089","type":"journal-article","created":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T16:09:14Z","timestamp":1781453354000},"source":"Crossref","is-referenced-by-count":0,"title":["Effects of\n                    <i>Chlorella sorokiniana<\/i>\n                    KU.B2 microalga on the development of\n                    <i>Culex quinquefasciatus<\/i>\n                    (Diptera: Culicidae) larvae"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5333-1538","authenticated-orcid":false,"given":"Jemisha","family":"Dudhat","sequence":"first","affiliation":[{"name":"Center of Excellence in Vector Biology and Vector-Borne Disease, Department of Parasitology, Faculty of Medicine, Chulalongkorn University , Bangkok,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8429-6889","authenticated-orcid":false,"given":"Jassada","family":"Saingamsook","sequence":"additional","affiliation":[{"name":"Parasitology and Entomology Research Cluster (PERC), Department of Parasitology, Faculty of Medicine, Chiang Mai University , Chiang Mai,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baby Kyi","family":"Soe","sequence":"additional","affiliation":[{"name":"Center of Excellence in Vector Biology and Vector-Borne Disease, Department of Parasitology, Faculty of Medicine, Chulalongkorn University , Bangkok,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuttha","family":"Sanevas","sequence":"additional","affiliation":[{"name":"Special Research Incubator Unit for Cryptogamic Biodiversity and Climate Responses, Department of Botany, Faculty of Science, Kasetsart University , Bangkok,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saowalak","family":"Kaewmee","sequence":"additional","affiliation":[{"name":"Department of Medical and Public Health Secretary, College of Allied Health Sciences, Suan Sunandha Rajabhat University , Samut Songkhram,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chonlada","family":"Mano","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Burapha University , Chonburi,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8801-7103","authenticated-orcid":false,"given":"Catherine","family":"Walton","sequence":"additional","affiliation":[{"name":"School of Earth and Environmental Sciences, Faculty of Science and Engineering, University of Manchester , Manchester,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9248-1678","authenticated-orcid":false,"given":"Padet","family":"Siriyasatien","sequence":"additional","affiliation":[{"name":"Center of Excellence in Vector Biology and Vector-Borne Disease, Department of Parasitology, Faculty of Medicine, Chulalongkorn University , Bangkok,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6755-8111","authenticated-orcid":false,"given":"George","family":"Dimopoulos","sequence":"additional","affiliation":[{"name":"Center of Excellence in Vector Biology and Vector-Borne Disease, Department of Parasitology, Faculty of Medicine, Chulalongkorn University , Bangkok,","place":["Thailand"]},{"name":"W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University , Baltimore, MD,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5338-6213","authenticated-orcid":false,"given":"Narissara","family":"Jariyapan","sequence":"additional","affiliation":[{"name":"Center of Excellence in Vector Biology and Vector-Borne Disease, Department of Parasitology, Faculty of Medicine, Chulalongkorn University , Bangkok,","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,6,13]]},"reference":[{"key":"2026061312494628700_tjag089-B1","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1007\/s11686-025-01112-0","article-title":"Larvicidal and pupicidal activity of neonicotinoid insecticide imidacloprid against southern house mosquito, Culex quinquefasciatus (say) (Diptera: Culicidae)","volume":"70","author":"Acharya","year":"2025","journal-title":"Acta Parasitol"},{"key":"2026061312494628700_tjag089-B2","first-page":"79","article-title":"Effect of ten chlorophytes on larval survival, development and adult body size of the mosquito Aedes aegypti","volume":"35","author":"Ahmad","year":"2004","journal-title":"Southeast Asian J. 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